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Bacillus amyloliquefaciens and application thereof

A technology of amylolytic spores and bacilli, applied in the fields of application, bacteria, and fungicides, can solve the problems of general biocontrol effects, single biocontrol objects, and unsuitable extraction, and achieve broad-spectrum plant pathogenic bacteria resistance and stable antibacterial Efficient effect, antibacterial range

Inactive Publication Date: 2012-06-06
中国农业科学院研究生院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It has been reported that the antibacterial substances synthesized by Bacillus amyloliquefaciens with biocontrol activity are extracellular substances, the concentration is low and it is not suitable for extraction, and the currently isolated Bacillus amyloliquefaciens has a single biocontrol object and the biocontrol effect is general

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1, the acquisition and identification of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YB-3 of the present invention

[0018] 1. Sample collection

[0019] Soil samples were collected from the courtyard garden of the Chinese Academy of Agricultural Sciences.

[0020] 2. Isolation and resistance screening of strains

[0021] (1) Strain dilution: Mix the samples taken evenly, weigh 1g and dissolve them in a conical flask filled with 10ml of distilled water, vibrate vigorously to make 10 -1 Soil suspension, then use a 1ml pipette gun to draw 1ml of the suspension into a test tube containing 9ml of distilled water, shake well to make 10 -2 soil suspension, diluted to 10 -8 ;

[0022] (2) Culture medium preparation: sterilize the prepared NA medium and PDA medium, pour into a sterile petri dish to cool, wherein the NA medium components are peptone 10g, beef extract 5g, NaCL 5g, agar powder 18g, Distilled water 1000ml, PH7.0; PDA medium components ar...

Embodiment 2

[0033] Embodiment 2, bacillus amyloliquefaciens YB-3 bacteriostatic spectrum is measured

[0034] Select common agricultural plant disease pathogens in rice sheath blight ACCC 36441, Phytophthora capsici ACCC36278, Fusarium wilt ACCC 36879, Gibberella wheat ACCC 31059, Botrytis cinerea ACCC30387, Sclerotinia sclerotiorum ACCC 36084, Aspergillus oryzae ACCC36443 , soybean root rot fungus ACCC36242, soybean anthracnose fungus ACCC36201, lettuce sclerotium ACCC36081, cucumber anthracnose fungus ACCC36065, cucumber gray mold ACCC 36448, rice blast fungus ACCC 37632, cotton Verticillium dahliae ACCC30309 (the above strains are all from Chinese agricultural microbial strains Preservation Management Center) was used as an indicator bacterium, and the mycelial growth rate method was used to determine its antibacterial effect. The specific method is as follows: under sterile conditions, culture 0.05ml of Bacillus amyloliquefaciens YB-3 culture solution (NB culture medium 37 ℃ to OD 66...

Embodiment 3

[0039] Embodiment 3, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YB-3 antibacterial substance distribution detection

[0040] Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YB-3 was cultured at 180rpm and 37°C for 16 hours (medium composition: 5g beef extract, 8g peptone, MgSO 4 1.4g, CaCO 3 0.8g, NaCL5g, distilled water 1000ml, pH6.8, 1000ml distilled water), then filter the culture solution with a 0.22 μm sterile filter, and collect the filtrate and thalli.

[0041] Under aseptic operation, the culture solution of rice sheath blight and Phytophthora capsici (PD medium, 150rpm, cultured at 25°C for 4d) was coated on the PDA plate, and then on the plate with a sterile puncher (6mm). Drill several holes, drop the collected bacteria (one bacterial loop) and filtrate (50 microliters) into the holes respectively, and place in an incubator at 25°C for 7 days.

[0042] The results show that there is no antagonistic active ingredient (no zone of inhibition) in ...

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Abstract

The invention discloses Bacillus amyloliquefaciens and an application thereof. The Bacillus amyloliquefaciens is Bacillus amyloliquefaciens YB-3, and the preservation number of the Bacillus amyloliquefaciens YB-3 in China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.4318. The Bacillus amyloliquefaciens YB-3 which can generate antibiotic substances which only exist in cells and are not diffused to a fermentation liquid and has the advantages of broad spectrum antibiotic range, and efficient and stable antibiotic effect can be developed into a microbe biocontrol preparation with various functions in the future.

Description

technical field [0001] The present invention relates to a strain of Bacillus amyloliquefaciens and its application, in particular to a newly screened strain of Bacillus amyloliquefaciens YB-3 (preservation number: CGMCC NO.4318), and the use of the strain in preventing and treating pathogenic fungi that can cause plant diseases Applications. Background technique [0002] In agricultural production, huge losses are caused by pests and diseases every year. Chemical pesticides have the advantages of high control effect, fast speed, wide insecticidal and bactericidal spectrum, low cost and easy use, so they have always played a leading role in the prevention and control of agricultural diseases and insect pests. Balance poses a great threat. [0003] In recent years, as people's demand for pollution-free, pure natural green food has increased, various developed countries have successively formulated development plans to ban chemical pesticides. Biopesticides have been widely ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 王海胜朱晓飞胡元森闫艳春许雷
Owner 中国农业科学院研究生院
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