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Manufacturing method of ceramide generation accelerator and/or glucosylceramide generation accelerator

A kind of ceramide, the technology of the manufacturing method, is applied in the field of ceramide and/or glucosylceramide production accelerator, achieves the effect of improving moisturizing ability

Inactive Publication Date: 2012-05-23
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, there is no report on the use of microorganisms of the genus Candida to produce ceramide and / or glucosylceramide production promoters.

Method used

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  • Manufacturing method of ceramide generation accelerator and/or glucosylceramide generation accelerator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (Manufacture of ceramide and / or glucosylceramide production promoter)

[0049] As a microorganism for producing ceramide and / or glucosylceramide production accelerator from bean dregs, Candida zeylanoides (purchased from Jiangnan University and preserved in CICIM, China University Industrial Microbiology Resources and Information Center, original number 472-20-1).

[0050] Inoculate the above strain obtained by culturing in YPD (Yeast Extract Peptone Dextrose Medium) medium at 30°C into a Erlenmeyer flask (capacity 300ml) containing 100ml of ceramide / glucosylceramide production promoter production medium, and inoculate at 30°C cultured for 72 hours. The medium contained 10 w / v% okara and 1 w / v% glucose. Wherein, the preparation of soybean dregs is obtained by soaking soybeans in water for 12 hours to make soybeans fully absorb water, then grinding and crushing the soybeans that have absorbed water, filtering and removing liquid components, and draining remaining resid...

Embodiment 2

[0055] As a microorganism for producing ceramide / glucosylceramide production accelerator from bean dregs, Candida zeylanoides (purchased from Jiangnan University and preserved in CICIM, China University Industrial Microbiology Resources and Information Center, original number 406- 20-1). The crude product of the ceramide and / or glucosylceramide production accelerator of the present invention (product B-1 of the present invention) was obtained by the same method as in Example 1, and then the soybean dregs fermented extract obtained by refining the crude product was used as the product of the present invention. Ceramide / glucosylceramide production enhancer.

[0056] Furthermore, the okara fermented extract was dissolved in 50v / v% ethanol, and its concentration was adjusted to 1w / v% (invention product B-2) and 0.1w / v% (invention product B-3), respectively, and stored in At 4°C, it was used in the subsequent ceramide / glucosylceramide production promotion test.

Embodiment 3

[0058] As a microorganism for producing ceramide / glucosylceramide production accelerator from bean dregs, Candida parapsilosis (purchased from Jiangnan University and preserved in CICIM, China Universities Industrial Microbiology Resources and Information Center, original number 514- 20-3). The crude product (product C-1 of the present invention) of the ceramide and / or glucosylceramide production accelerator of the present invention was obtained by the same method as in Example 1, and then the soybean dregs fermented extract obtained by refining the crude product was used as the okara fermented extract of the present invention. Ceramide / glucosylceramide production enhancer.

[0059] Furthermore, the okara fermented extract was dissolved in 50v / v% ethanol, adjusted to a concentration of 1w / v% (product C-2 of the present invention), stored at 4°C, and used for the subsequent production of ceramide / glucosylceramide Facilitate testing.

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Abstract

The invention provides a manufacturing method of a ceramide generation accelerator and / or a glucosylceramide generation accelerator, wherein Candida microorganisms are cultured in a culture medium containing beam dregs and saccharides, and the ceramide generation accelerator and / or the glucosylceramide generation accelerator are / is obtained from an obtained supernatant. According to the manufacturing method disclosed by the invention, by utilization of specific microorganisms and byproducts, namely the bean dregs, of the food processing industry and the like as a main raw material of the culture medium, the ceramide generation accelerator and / or the glucosylceramide generation accelerator can be acquired at low cost and high efficiency.

Description

technical field [0001] The present invention relates to a method for producing a ceramide and / or glucosylceramide production promoter capable of promoting the production of ceramide and / or glucosylceramide in the skin by using specific microorganisms and using bean dregs, a by-product of the food processing industry, etc., as the main raw material of a culture medium . Background technique [0002] Ceramide, one of the sphingolipids, exists in the stratum corneum of the skin and accounts for about 50% of the intercellular lipids in the stratum corneum. Sphingolipid is a general term for complex lipids having a sphingosine skeleton. Glucosylceramide is a kind of sphingolipid formed by binding glucose to ceramide. The ceramide synthesized by epidermal cells is temporarily stored in the form of glucosylceramide or sphingomyelin; it is then excreted outside the cell, where glucocerebrosidase (Glucocerebrosidase) and sphingomyelinase (Sphingomyelinase) Under the action of cera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A61K8/99A61Q19/00C12R1/72C12R1/73C12R1/74
Inventor 孔凡旗
Owner KAO CORP
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