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Solid fermentation bioreactor

A bioreactor, solid-state fermentation technology, applied in solid-phase fermentation bioreactor, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc., can solve the problems of fermentation repeatability and poor fermentation effect, Fermentation conditions are difficult to control, mass transfer and heat transfer are not uniform, etc., to achieve the effect of isolating the entry of external pollutants, saving manpower and improving fermentation efficiency

Inactive Publication Date: 2013-05-01
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a solid-state fermentation bioreactor in order to solve the problems of uneven mass transfer and heat transfer, difficult control of fermentation conditions, poor fermentation repeatability and fermentation effect in the solid-state fermentation process

Method used

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Examples

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Embodiment 1

[0070] Following is main raw material with kelp slag, utilizes above-mentioned solid-state fermentation device to produce Bacillus thuringiensis (Bt) biopesticide, sets forth the method utilizing this solid-state fermentation bioreactor to carry out solid-state fermentation:

[0071] (1) Dried kelp, crushed to 40 mesh, added water to adjust the initial water content to 75%, adjusted the pH to 7.0-7.4, sterilized by moist heat for 20-30 minutes, and cooled to about 30°C;

[0072] (2) Bacterial strain expansion culture: pick a ring of Bacillus thuringiensis bacterial lawn from the slant medium of Bacillus thuringiensis, inoculate in 100mL sterilized beef extract peptone liquid medium (beef extract 0.3%, peptone 1%, NaCl 0.2%, pH 7.0-7.2), for shake flask culture. The culture conditions are: temperature 30°C, rotation speed 200 rpm, culture for 7-8 hours, as Bt bacteria seed liquid;

[0073](3) Solid-state fermentation: start the fermentation equipment, and sterilize by ultravio...

Embodiment 2

[0077] Taking bran as the main raw material, the above-mentioned solid-state fermentation device is utilized to produce metarhizium anisopliae biopesticides, and the method for utilizing this solid-state fermentation bioreactor to carry out solid-state fermentation is set forth below:

[0078] (1) Add water to the bran to adjust the initial water content to 60%, adjust the pH to 7.0, sterilize with damp heat for 20 to 30 minutes, and cool to about 26°C;

[0079] (2) Strain expansion culture: seed culture was added to 250mL Erlenmeyer flask with 100mL of culture solution, after sterilization, inoculated with 3mL of Metarhizium anisopliae spore liquid, at 26°C, 150r / min shaking culture for 3 days, then used as Metarhizium anisopliae seed liquid;

[0080] (3) Solid-state fermentation: start the fermentation equipment, and sterilize by ultraviolet lamp irradiation for 30 minutes. Set the temperature, humidity, and ventilation as temperature 26°C, humidity 80%, and air flow 1:1 to ...

Embodiment 3

[0084] Below with asparagus leftovers as main raw material, utilize above-mentioned solid-state fermentation device to produce Beauveria bassiana biological pesticide, set forth the method utilizing this solid-state fermentation bioreactor to carry out solid-state fermentation:

[0085] (1) Add water to the asparagus leftovers to adjust the initial water content to 60-65%, adjust the pH to 8.0, sterilize with moist heat for 20-30 minutes, and cool to about 30°C;

[0086] (2) Strain expansion culture: 50ml of seed culture medium was installed in a 250ml Erlenmeyer flask, inoculated with an activated slant, and cultivated on a shaking table at a temperature of 27°C and a rotating speed of 200r / min for 48h;

[0087] (3) Solid-state fermentation: start the fermentation equipment, and sterilize by ultraviolet lamp irradiation for 30 minutes. Set the temperature, humidity, and ventilation to 27°C and 85% humidity respectively. After the system stabilizes for 10 minutes, inoculate th...

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Abstract

The invention relates to a solid fermentation bioreactor. The reactor comprises a reaction casing , and a porous material layer separator plate (5) is arranged in the reaction casing to divide the reaction casing into an upper fermentation chamber (1) and a lower layer air chamber (3); the reactor also comprises a fermentation control system for controlling a stirring unit, a temperature control unit, a humidity control unit, an air feed control unit and a fermentation tail gas detection and processing unit, so as to realize real time monitoring and control on temperature, humidity, ventilation, stirring and tail gas. The invention realizes totally enclosing on-line fermentation and prevents extraneous pollutants from entering; the tail gas detection system carries out real time on-line monitoring on fermentation gas, and tail gas is treated by tail gas recovery equipment to protect the environment, thereby realizing coupling operation of temperature, humidity, ventilation, stirring and tail gas control system and ensuring necessary essential factors for microbe growth.

Description

technical field [0001] The invention relates to the technical field of fermentation equipment, in particular, the invention relates to a solid-state fermentation bioreactor. Background technique [0002] Solid state fermentation (Solid State Fermentation, SSF), also known as solid fermentation, refers to the process in which microorganisms grow, reproduce, and metabolize on a solid wet medium with no or almost no free water. Solid-state fermentation has the characteristics of low investment, almost no discharge of three wastes (waste water, waste gas, solid waste), low energy consumption, high yield, short fermentation time, and low cost, which cannot be compared with liquid-state fermentation, which has attracted widespread attention. The research in the field of solid-state fermentation and its application in environmental resources has made great progress, mainly including applications in biofuels, biopesticides, biological growth agents, biofertilizers, and biodetoxifica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/18C12M1/02C12M1/36C12R1/07C12R1/645
CPCC12M21/16C12M23/34C12M27/06C12M41/12C12M41/34C12M47/10
Inventor 赵兵赵庆生袁晓凡王晓东
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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