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Sialidase detection kit

A detection kit and sialic acid technology, applied in the field of medical testing, can solve the problems of the influence of the NADH degradation rate on the performance of the reagent, the inability to meet the needs of clinical testing, etc., and achieve the effects of easy popularization and application, easy operation, and simple method.

Inactive Publication Date: 2012-04-04
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The enzyme analysis method has the advantages of high specificity, simple and fast operation, accurate and safe, and automatic analysis. However, because the reducing coenzyme NADH used in the reagent is unstable under this reaction condition, it must be made into a dry powder reagent for long-term storage and stability. It must be used as soon as possible after dissolving, otherwise the performance of the reagent will be greatly affected due to the rapid degradation of NADH during the storage process, and it cannot meet the needs of clinical testing.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The enzymatic sialic acid detection kit consists of the following two reagents, of which:

[0032] Reagent 1:

[0033]

[0034]

[0035] Reagent 2:

[0036]

[0037] The preparation method of Reagent 1 and Reagent 2 is a conventional method, that is, the components of Reagent 1 and Reagent 2 are added to distilled water and then mixed and stirred evenly.

Embodiment 2

[0039] The enzymatic sialic acid detection kit consists of the following two reagents, of which:

[0040] Reagent 1:

[0041]

[0042] Reagent 2:

[0043]

[0044] The preparation method of the kit in Example 2 is the same as in Example 1.

Embodiment 3

[0046] The enzymatic sialic acid detection kit consists of the following two reagents, of which:

[0047] Reagent 1:

[0048]

[0049]

[0050] Reagent 2:

[0051]

[0052] The preparation method of the kit of Example 3 is the same as that of Example 1.

[0053] The test conditions for the enzymatic sialic acid detection kit of the present invention to measure sialic acid in a sample are as follows: temperature: 30-37° C.; cuvette light path: 1.0 cm. The main detection wavelength is 340nm, and the secondary wavelength is 405nm.

[0054] The method for measuring sialic acid in a sample using the assay kit of the present invention is as follows: add reagent 1 to the sample (calibration tube as the sample) and mix evenly, incubate at 30-37°C for 3-5min, add reagent 2 and mix evenly, and then add reagent 2 and mix evenly at 30-37°C After reacting for 90s, monitor the change of absorbance for 90s. The sample volume is 8 μl, the reagent 1 volume is 240 μl, and the reage...

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Abstract

The invention provides a sialidase detection kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 comprises the components of buffer solution, neuraminidase, lactic dehydrogenase, surfactant, stabilizer and preservative; and the reagent 2 comprises the components of buffer solution, reducing coenzyme, neuraminic acid aldolase, stabilizer and preservative. The kit has accurate detection and low cost and is convenient, applicable, stable and reliable.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to an enzymatic sialic acid detection kit. Background technique [0002] N-Acetylneuraminic Acid (NANA), commonly known as Sialic acid (SA), is one of many natural neuraminic acid derivatives, and its parent structure is an endocyclic amino acid composed of nine carbon atoms , is a general term for a family of compounds, which got its name because it was originally isolated from submandibular gland mucin. [0003] Sialic acid is distributed in mammals, especially in vascular endothelial cells. The total sialic acid (TSA) content in human serum is about 1.5-2.5 mmol / L, including free sialic acid and bound sialic acid. Among them, the content of free sialic acid is relatively low, about 1-3 μmol / L; sialic acid bound to lipoproteins and glycoproteins is bound sialic acid, which is the main form of sialic acid in serum. Sialic acid is located at the end of oligosaccharide cha...

Claims

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Application Information

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IPC IPC(8): C12Q1/527C12Q1/34C12Q1/32
Inventor 邹炳德沃燕波
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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