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Environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture

A compound microorganism and environment-friendly technology, applied in biological water/sewage treatment, chemical instruments and methods, water/sludge/sewage treatment, etc. The effect of stabilizing the water color

Active Publication Date: 2011-10-19
WUHAN HUAYANG ANIMAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the current aquatic microbial preparation manufacturers who mainly produce single microbial preparations or two or three kinds of probiotic compound preparations, which can only solve a certain aspect of the breeding process, and the dosage is very large, ranging from 200 grams to 2 per mu. Therefore, in the face of complex environmental problems in the breeding process, it is necessary to choose different types of microbial preparations and use a larger amount, which is a big challenge in terms of use costs. Insufficient, providing an environmentally friendly compound Microbial preparation and its preparation and its application in aquaculture

Method used

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  • Environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture

Examples

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Effect test

Embodiment 1

[0035] Example 1 Preparation of high-efficiency composite microbial water purifier

[0036] A kind of preparation of environment-friendly compound microbial water purifying agent, its method is as follows:

[0037] (1) High-density liquid fermentation of single bacillus

[0038] The selected strains were inoculated into LB liquid seed tanks, fermented at 37°C for 16 hours, and then transferred to a fermentation culture tank optimized by the culture medium at a 2.5% inoculum size, and the reducing sugar, ammonia nitrogen concentration and dissolved oxygen were monitored in real time. Feed when the reducing sugar drops to 0.5%, add 5% glucose. Ferment for 48 hours, and when the yield of spores reaches more than 95%, the fermentation ends. The fermentation broth is spray-dried, and 7.5% of the biological protection agent bran is added to make 1000-5000 super concentrated raw powder.

[0039] (2) According to the simulation experiment and the physiological function characterist...

Embodiment 2

[0041] Implementation 2 Preparation of high-efficiency composite microbial water purifier

[0042] A kind of preparation of environment-friendly compound microbial water purifying agent, its method is as follows:

[0043] (1) High-density liquid fermentation of single bacillus

[0044] The selected strains were inoculated into LB liquid seed tanks, fermented at 37°C for 12 hours, and then transferred to the fermentation culture tank optimized with the culture medium at 5% inoculum size, and the reducing sugar, ammonia nitrogen concentration and dissolved oxygen were monitored in real time. When the reducing sugar is reduced to 0.5% feeding, add 10% fermentation medium. Ferment for 24 hours, and when the yield of spores reaches more than 95%, the fermentation ends. The fermented liquid is spray-dried, and 10% of cornstarch, a biological protection agent, is added to make 1000-5000 ultra-concentrated raw powder.

[0045] (2) According to the simulation experiment and the phys...

Embodiment 3

[0047] Example 3 Application of microbial water purifier in culture of Penaeus vannamei

[0048] Two shrimp ponds with similar ecological environment characteristics were selected for the experiment. The area is 2 mu, the water depth is 1.5 meters, and the previous treatment is the same. Divided into control group and test group. The control group did not receive any treatment, and the test group sprayed 20 g / mu·m of environmentally friendly compound microbial water purifier directly near the aerator, and started the aerator for 1 hour.

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Abstract

The invention relates to an environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture. An environment-friendly composite microbial water purifying agent comprises the following components in percentage by weight: 20 to 40 percent of bacillus subtilis, 10 to 20 percent of bacillus licheniformis, 10 to 15 percent of bacillus megaterium, 10 to 15 percent of brevibacillus laterosporus, 5 to 10 percent of bacillus pumilus, 5 to 20 percent of bacillus coagulans and 2 percent of biological activating agent. The high-concentration composite microbial preparation is prepared by screening eight strains of probiotics by simulating the physiological functions of normal microbial flora in an aquaculture environment and through scientific compatibility, so that division and cooperation amount the bacterial strains can be realized, a biological enhancement technology can be combined with an indigenous flora activating technology, complex problems in a culture water body can be solved completely, the using amount is small, the cost is low, and the competitive advantage is remarkable.

Description

technical field [0001] The invention relates to an environment-friendly compound microbial preparation, its preparation and its application in aquaculture. Background technique [0002] With the intensive and high-density development of aquaculture, high feeding rate and high fertilization amount have become one of the means that must be implemented. However, the resulting eutrophication, water quality deterioration, and the spread of explosive diseases are endangering the healthy development of the aquaculture industry. How to solve these contradictions in aquaculture, microbial enhancement technology has become the most promising application technology. [0003] In the natural state, the indigenous bacteria in the water body can maintain the normal material circulation of the water quality through self-purification. However, with the development of high-density aquaculture, the organic load in the water body has exceeded the scope of the self-purification ability of micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
Inventor 张金林凌华云马立保孙慧
Owner WUHAN HUAYANG ANIMAL PHARMA
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