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Method for determining phenylpropanoid glycoside substances in Tibetmedicine Lamiophlomis rotata

A technology for the determination of phenylpropanoid glycosides and methods, applied in the field of analytical chemistry, can solve problems such as limitations, lack of reference substance determination, and difficulty in preparation, and achieve the effects of easy standardization, good application value, and easy access

Inactive Publication Date: 2013-11-27
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the labor-intensive and time-consuming work of purifying and preparing the phenylpropanoid reference substance from the unique plant, the cost is high, and the preparation of some low-content phenylpropanoid reference substances in the unique plant is very difficult, so this The determination method is often limited by the absence of a reference substance. Usually, only a few phenylpropanoid glycosides in Duyiwei can be measured, which cannot fully reflect the content of phenylpropanoid glycosides in Duyiwei.

Method used

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  • Method for determining phenylpropanoid glycoside substances in Tibetmedicine Lamiophlomis rotata
  • Method for determining phenylpropanoid glycoside substances in Tibetmedicine Lamiophlomis rotata

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Sample pretreatment: Accurately weigh 10.0g of pulverized Duyiwei whole grass and add it to a 500ml flask, heat and reflux with 100ml distilled water for extraction, a total of 3 extractions. Combine the extracts obtained three times, filter with gauze, and concentrate the filtrate to 30ml by distillation with a rotary evaporator, add 10% hydrochloric acid for hydrolysis, adjust the concentration of hydrochloric acid to 2mol / L, obtain a hydrolyzate after hydrolysis for 2 hours, and filter to constant volume to 50ml, accurately measure 10ml, then dilute 10 times with methanol and set the volume in a 100ml volumetric flask to obtain a sample solution of Duyiwei whole herb for sample analysis.

[0040]Reference substance solution preparation: Accurately weigh 1.54g of hydroxytyrosol with a purity of 98%; 1.68g of homovanillyl alcohol with a purity of 95%; 1.82g of 3,4-dimethoxyphenethyl alcohol with a purity of 95%; 96% caffeic acid 1.80g; purity is 97% ferulic acid 1.94g;...

Embodiment 2

[0043] Sample pretreatment: Accurately weigh 6.72g of pulverized Duyiwei tablets and add them to a 250ml flask, add 25ml of distilled water and 25ml of methanol to heat and reflux for extraction, and extract 3 times in total. Combine the extracts obtained for three times, filter through filter paper, and concentrate the filtrate to 20ml by distillation with a rotary evaporator, add 10% hydrochloric acid for hydrolysis, adjust the concentration of hydrochloric acid to 2.5mol / L, obtain a hydrolyzate after hydrolysis for 1 hour, and determine When the volume reaches 50ml, accurately measure 10ml, then dilute it 10 times with methanol and place it in a 100ml volumetric flask to obtain a sample solution of Duyiwei Tablet preparation for injection analysis.

[0044] Reference substance solution preparation: Accurately weigh 1.54g of hydroxytyrosol with a purity of 98%; 1.68g of homovanillyl alcohol with a purity of 95%; 1.82g of 3,4-dimethoxyphenethyl alcohol with a purity of 95%; 9...

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Abstract

The invention discloses a method for determining phenylpropanoid glycoside substances in a Tibetan medicine Lamiophlomis rotate. According to the invention, the characteristic that phenylpropanoid glycoside can be hydrolyzed to release phenethanol and cinnamic acid compounds is used, various types of phenethanol and cinnamic acid compounds in Lamiophlomis rotata are separated by a high performance liquid chromatography method and determined by using an external standard method, so that accurate determination of phenylpropanoid glycoside substances in the Lamiophlomis rotate can be achieved.

Description

technical field [0001] The invention relates to a technique for separating and measuring phenylpropanoid glycosides in the unique Tibetan medicine and its preparation, and belongs to the technical field of analytical chemistry. Background technique [0002] Duyiwei is a plant of the Labiatae family, which grows in alpine meadows at an altitude of 2,700 to 4,500 meters. It is widely distributed in plateau areas such as Tibet, Gansu, and Sichuan. It is a folk medicine of the Tibetan, Mongolian, and Naxi ethnic groups. It has the effects of removing blood stasis, antibacterial and anti-inflammatory, etc. It is mainly used clinically to treat bruises, traumatic bleeding, rheumatic arthralgia, and yellow water disease. [0003] The unique plant mainly contains flavonoids, iridoids, and phenylpropanoids. Chinese Patent No. 200510035066.5 provides an injection made by extracting and separating raw materials containing phenylpropanoid glycosides as the main active ingredient, invol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/53G01N30/02G01N30/06A61P19/00A61P29/00A61P17/02A61P7/04A61P31/04
Inventor 赵亮董树清刘芹
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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