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Spleen immune factor and its preparation method

A technology of immune factors and spleen, applied in the field of biomedicine, to achieve the effect of expanding utilization efficiency, low biological activity, and enhancing immune function

Inactive Publication Date: 2012-04-04
曹吉祥
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After research and exploration, the present invention has extracted animal spleen immune factors. After searching, there is no relevant literature report on spleen immune factors in the prior art.

Method used

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  • Spleen immune factor and its preparation method
  • Spleen immune factor and its preparation method
  • Spleen immune factor and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1 Preparation of spleen immune factor by ultrafiltration

[0031] The fresh and healthy spleen that has passed the quarantine is washed repeatedly with sterile pure water to clean the grease and fascia on the surface of the spleen; the spleen is cut into pieces and crushed in a high-speed tissue grinder, and put into the cold storage after being divided into plates (- 25℃~-30℃) quick freezing, freezing and thawing 3 to 5 times, adding 3 to 5 times of sterile pure water, mixing, then emulsifying in a colloid mill, centrifuging to separate solids and extracts, 15,000 rpm; The filtrate is collected in a container, and the filtrate is filtered; finally, the ultrafiltration product with a molecular weight cut-off of 20,000 Daltons is the spleen immune factor. It is a mixture with a molecular weight of less than 20,000 Daltons.

Embodiment 2

[0032] Example 2 Preparation of Specific Spleen Immune Factors by Homogenate Dialysis

[0033] Take the preparation of specific spleen immune factors of tumor tissue as an example. First, the fresh tumor tissue was repeatedly homogenized, frozen and thawed to completely break up the tumor cells, and then used to immunize healthy pigs that had passed quarantine. After the immunization is successful, take the aseptic homogenate of pig spleen and freeze-thaw it 3 to 5 times to completely destroy the cells, then put the homogenate freeze-thaw solution into a dialysis bag for dialysis, and collect the part with a molecular weight less than 20,000 Daltons, namely For the specific spleen immune factor.

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Abstract

Belonging to the technical field of bio-medicine, the invention discloses a spleen immune factor and its preparation method. The spleen immune factor or specific spleen immune factor is prepared through an ultrafiltration or dialysis method. With a molecular weight less than 20000 Dalton, the spleen immune factor of the invention has proteins absent in a transfer factor as well as high bioactivity. Development and application of the spleen immune factor provided in the invention not only expands the utilization efficiency of animal (chicken, pig etc.) spleen resources, enhances the bioactivity of a spleen extract, but also provides a preparation raw material characterized by easily available crude materials, low cost and no toxic and side effect and used for treating human as well as animal refractory diseases like bird flu and tumors, etc. Therefore, the spleen immune factor of the invention is suitable for industrial production.

Description

technical field [0001] The invention relates to immune factors, in particular to spleen immune factors and a preparation method thereof, and belongs to the technical field of biomedicine. Background technique [0002] The spleen is the largest lymphatic organ in humans and animals; the spleen is also the central immune organ of the body. The immunological function of the spleen is mainly reflected in the production of immune active cells, recognition of antigens, and production of antibodies. T lymphocytes and B lymphocytes in the spleen can produce immune active substances such as immunoglobulin and complement, as well as various active factors such as interferon and transfer factor. When antigens or foreign substances such as germs, viruses, and protozoa appear in the blood, these active cells and active substances in the spleen can play an active or passive immune role, engulfing, neutralizing or killing the bacteria, viruses and other foreign substances entering the bod...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K38/48A61P37/04A61K35/26
Inventor 曹吉祥
Owner 曹吉祥
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