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Biochemistry method for researching white spirit omagari microbial community structure

A microbial community and biochemical technology, applied in biochemical equipment and methods, microbial measurement/inspection, scientific instruments, etc., can solve problems such as inability to reflect the overall composition of living microorganisms, stabilize the quality of Daqu, and improve the production process of Daqu Effect

Active Publication Date: 2012-03-21
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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Problems solved by technology

[0008] The purpose of the present invention is to provide a biochemical method for studying the microbial community structure of liquor Daqu without cultivating, so as to solve the problem that the composition of living microorganisms (including non-culturable microorganisms) cannot be reflected in the current research on the microbial community of Daqu.

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  • Biochemistry method for researching white spirit omagari microbial community structure
  • Biochemistry method for researching white spirit omagari microbial community structure

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Embodiment Construction

[0020] For a better understanding of the present invention, it is described in conjunction with examples.

[0021] Taking different batches and different types of Daqu from a certain production area as the experimental object, crush the koji pieces under aseptic conditions, pass through a 20-mesh sieve, and set aside; take 5g of Daqu powder and add 15mL of extract to a 50mL centrifuge tube wrapped in tin foil (Chloroform, methanol and citric acid buffer solution = 1:2:0.8, V:V:V), shake well, and stand at low temperature overnight. Continue to add 7.5mL chloroform and 7.5mL phosphate buffer solution to the centrifuge tube, shake well and let stand at low temperature again overnight; centrifuge at room temperature at 6000r / min for 10min, carefully suck off the supernatant with a disposable sterile pipette, and filter the lower layer with filter paper until In a small Erlenmeyer flask wrapped in tin foil, dry the resulting liquid with high-purity nitrogen; add 300 μL of chlorofo...

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Abstract

The invention relates to a biochemistry method for researching white spirit omagari microbial community structure, namely phospholipid fatty acid spectrogram technology. The biochemistry method comprises the following main steps: firstly, weighing certain mass of omagari; secondly, adding extracting solution and organic solvent, thus obtaining organic phase containing lipid; thirdly, utilizing the organic solvent for eluting the organic phase by a silicon pillar; fourthly, collecting eluent containing polar lipid; fifthly, conducting methyl esterification reaction on the obtained eluent under the weak base condition, thus obtaining fatty acid methyl ester; sixthly, utilizing a gas chromatograph-mass spectrometer for nature determining and quantifying on the fatty acid methyl ester; and seventhly, calculating the biomass live weight of living microorganism of characterization of the phospholipid fatty acid. The biochemistry method provides an accurate and fast technical measure for the white spirit omagari microbial community structure and related researching, controls the production process quality of the omagari, and has important and practical meaning of promoting and stabilizing the quality level of the white spirit omagari.

Description

Technical field: [0001] Phospholipid fatty acid (PLFA) spectrum analysis is a biochemical method for quantitative analysis of microorganisms, mainly used to study the diversity of microorganisms in complex communities. In microbial ecology research, PLFA spectrum analysis method is mainly used to detect environmental samples. Research on microbial biomass, community structure, nutritional status and metabolic activity. Background technique: [0002] Daqu is a carrier for collecting, enriching, and cultivating beneficial microorganisms and their metabolites. As a saccharification agent, fermentation agent, and flavoring agent in the brewing process of liquor, it has a great impact on the yield, quality, and quality of liquor. , is an important material guarantee for traditional solid-state fermentation and distillation of Daqu liquor. The microbial community structure of Daqu determines its metabolic function. Changes in the microbial community structure will lead to changes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06G01N30/00
Inventor 熊正河钟其顶孟镇
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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