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Tumor targeted molecule and application thereof

A tumor-targeting molecule and tumor technology, which is applied in the direction of anti-tumor drugs, non-active ingredients of polymer compounds, preparations for in vivo tests, etc., can solve problems such as toxic side effects, low tumor targeting, and large drug doses

Inactive Publication Date: 2012-03-14
杨静雯
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The contrast agents currently used in clinical practice mainly have problems such as high dosage, low tumor targeting, and certain toxic and side effects.

Method used

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  • Tumor targeted molecule and application thereof
  • Tumor targeted molecule and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0029] Example 2. Preparation of human ferritin containing iron oxide core This preparation process is always at N 2 environment. Add 8 ml of degassed solution containing 100 mM NaCl to N 2 Add 2ml of HFn with a concentration of 1mg / ml to a total of 2mg of samples (also dissolved in 100mM NaCl solution) into the protected airtight container, and titrate the pH of the system to 8.5 with 50mM NaOH while the circulating water maintains an ambient temperature of 65°C; Add concentration of 12.5mM (NH4) at a molar ratio of 3000 iron atoms in a cage protein cavity 2 Fe(SO4) 2 , and press H at the same time 2 o 2 :Fe(II) at a molar ratio of 1:3 was added with freshly prepared degassed H 2 o 2 ; Fe(II) and H 2 o 2 Add to the reaction system at a fixed rate of 31.3ul / min, and at the same time use 50mM NaOH to titrate and stabilize the pH of the system at 8.5. After the reaction was completed, 200ul of 300mM sodium citrate was added to chelate excess free iron ions, and human ...

Embodiment 3

[0030] Example 3. MRI Scanning Imaging of Tumor-targeted Contrast Agent in Human Tumor-bearing Nude Mouse Model Human lung cancer cell line SPCA1 was inoculated subcutaneously in nude mice (10 7 cells / mouse), and a total of 10 human-bearing lung cancer nude mice models were made. They were divided into two groups with 5 rats in each group, namely the observation group and the control group. When the tumors grow to 1-1.5cm in size, all nude mice were scanned by MRI, and the MRI signal intensity of the tumor lesions was determined to be 278. HFn ferritin, the nude mice of the control group were injected with Fe with a diameter of 50nm at a ratio of 5mgFe / kg body weight. 3 o 4 Magnetic nanoparticles, MRI enhanced scan was performed half an hour later, and the MRI signal intensity of tumor lesions in the observation group was determined to be 451, while the MRI signal intensity of tumor lesions in the control group was 325.

[0031] Human liver cancer cell line hep3b was inoc...

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Abstract

The invention provides a tumor targeted molecule capable of specifically binding to a plurality of tumors, i.e., ferritin, and a contrast agent for diagnosis of tumor or a therapeutic drug containing the tumor targeted molecule. The contrast agent or the therapeutic drug has the characteristics of no or low toxicity, strong specificity, a small utilization amount, high stability in vivo, etc, and is especially applicable to targeted diagnosis or treatment of malignant tumor tissue, e.g., liver cancer tumor, melanin tumor, lung cancer tumor, breast cancer tumor, colonic / rectal cancer tumor, pancreas cancer tumor, stomach cancer tumor, ovarian cancer, head and neck cancer, neuroglioma, etc.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. Specifically, the invention provides a novel tumor targeting molecule, which is ferritin assembled from 24 protein subunit molecules, and the protein subunit molecule is human ferritin heavy and light chains. They can be assembled into a cage-like cavity structure of 24 subunits according to various molecular ratios, at least containing heavy chain subunits. The present invention also provides that the tumor targeting molecule can specifically recognize and bind to liver cancer tumors; melanoma tumors; lung cancer tumors; breast cancer tumors; colorectal cancer tumors; pancreatic cancer tumors; gastric cancer tumors; ovarian cancer, glioma and other malignant tumors tissue, the present invention also provides a pharmaceutical composition comprising the tumor targeting molecule; the present invention also provides a contrast agent for ultrasound imaging diagnosis and magnetic resonance imaging ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47A61K47/42A61K49/14A61P35/00
Inventor 杨静雯
Owner 杨静雯
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