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Surface modification method of nanometer hydroxyapatite mediated by APTS

A nano-hydroxyapatite and surface modification technology, applied in recombinant DNA technology, medical science, prosthesis, etc., can solve the problem of lack of comprehensive evaluation of cytotoxicity and blood compatibility, and achieve easy operation and increase the number of , the effect of enhancing modifiability

Inactive Publication Date: 2012-02-22
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] But so far, there is no literature report on APTS surface modification and further functional modification of rod-shaped n-HA, and there is no comprehensive evaluation of its cytotoxicity and blood compatibility.

Method used

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  • Surface modification method of nanometer hydroxyapatite mediated by APTS
  • Surface modification method of nanometer hydroxyapatite mediated by APTS
  • Surface modification method of nanometer hydroxyapatite mediated by APTS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Mix 190mg of n-HA with 200mL of absolute ethanol, supplemented by ultrasonic dispersion (80W, 1.5h), to prepare n-HA / ethanol suspension; slowly add 200μL APTS to the above n-HA / ethanol suspension, And under the condition of room temperature supplemented with magnetic stirring reaction for 12h, the reaction ended. The suspension obtained by the reaction was centrifuged (6000r / min, 5min), washed (2 times with distilled water, 3 times with absolute ethanol), separated and precipitated to obtain n-HA-APTS nanoparticles.

Embodiment 2

[0056] Mix 200mg n-HA with 200mL absolute ethanol, supplemented by ultrasonic dispersion (50W, 2h), to prepare n-HA / ethanol suspension. Add 5 mL of ultrapure water dropwise to the above n-HA / ethanol suspension. After uniform magnetic stirring, 235 μL APTS was slowly added dropwise to the above n-HA / ethanol suspension, and reacted for 12 hours at room temperature with the aid of magnetic stirring, and the reaction was completed. The suspension obtained by the reaction was centrifuged (6000r / min, 5min), washed (2 times with distilled water, 3 times with absolute ethanol), separated and precipitated to obtain n-HA-APTS nanoparticles.

Embodiment 3

[0058] 200 mg of n-HA-APTS nanoparticles prepared in Example 1 were uniformly dispersed in 15 mL of DMSO by ultrasonic assistance, and 1 mL of triethylamine was slowly added dropwise under the condition of magnetic stirring, and stirring was continued for 30 min to make it mix with n-HA -APTS dispersion mixed thoroughly. Then, 500 μL of DMSO solution containing 50 μL of acetic anhydride was slowly added, and the reaction was carried out at room temperature for 24 h with the aid of magnetic stirring, and the reaction was completed. The suspension obtained by the reaction was centrifuged (6000r / min, 5min), washed (2 times with distilled water and 3 times with absolute ethanol), separated and precipitated to obtain n-HA-APTS.Ac nanoparticles.

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Abstract

The invention relates to a surface modification method of nanometer hydroxyapatite mediated by APTS, which includes (1) mixing the nanometer hydroxyapatite n-HA with ethanol and dispersing the mixture through ultrasonic wave to obtain n-HA ethanol suspending liquid; (2) adding the APTS into the n-HA ethanol suspending liquid in dropwise mode and conducting centrifuging, washing, separating and precipitating on the suspending liquid after reaction is finished under room temperature condition to obtain n-HA-APTS nanometer particles; or further conducting acetylation or carboxylation on the n-HA-APTS nanometer particles. The surface modification method is mild in condition and simple in process. Products are easy to separate, and the used modification agent is environment-friendly silane materials. The obtained n-HA-APTS, n-HA-APTS.AC and n-HA-APTS.SAH nanometer particles have different active groups on the surfaces, have good biocompatibility and blood cell stability and are wide in application prospect.

Description

technical field [0001] The invention belongs to the field of modifying the surface of nanometer hydroxyapatite, in particular to a method for modifying the surface of nanometer hydroxyapatite mediated by APTS. Background technique [0002] In recent years, the application of nanoparticles in biomedicine, electrochemical sensors, molecular imaging and other fields has received extensive attention. Nanohydroxyapatite (Nanohydroxyaptite, n-HA, molecular formula: Ca 10 (PO 4 ) 6 (OH) 2 ) is the main inorganic component of mammalian teeth and bones, and has excellent properties such as good biological activity and osteoconductivity. n-HA has a very large specific surface area, which enables it to adsorb active substances such as proteins, lipids, and polysaccharides; and n-HA can also perform ion exchange with the surrounding liquid environment. These excellent properties of n-HA make it have broad application prospects in gene transfection, bone tissue engineering, drug car...

Claims

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Application Information

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IPC IPC(8): A61L27/12A61L27/50C12N15/09
Inventor 史向阳王世革
Owner DONGHUA UNIV
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