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Preparation method of rabdosia japonica total diterpene

A technology of blue calyx fragrant tea and total diterpenes, which is applied in the field of natural medicinal chemistry, can solve the problems of long separation cycle and high cost, and achieve the effects of high product quality, simple operation and low energy consumption

Inactive Publication Date: 2012-01-25
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the method of extracting diterpenoids from the medicinal material S. japonica is mostly separated and purified by organic solvent extraction and silica gel column chromatography. This method has the disadvantages of long separation period and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Grind the leaves of S. japonica into a coarse powder, weigh 1 kg and put it in an extraction tank, add 6 times the amount of 70% methanol to heat and reflux for extraction for 2 hours, extract twice, combine the two extracts, let cool, filter, The filtrate is heated and stirred with 1% medicinal activated carbon, filtered, the decolorized filtrate is concentrated to a relative density of 1.08, and then passed through a hollow fiber ultrafiltration membrane system with a molecular weight cut-off of 6000 Daltons, and the operating pressure is adjusted to 0.2MPa. After passing through the liquid, load the permeate on the pretreated macroporous adsorption resin column. After saturated adsorption, it is eluted with water and 60% ethanol in sequence, and the 60% ethanol eluate is collected, concentrated by rotary evaporation, and dried to obtain the calyx calyx The total diterpenoids of the fragrant tea, the content of the total diterpenoids is 76%. After the HPLC detection, t...

Embodiment 2

[0014] Grind the blue calyx calyx leaves into coarse powder, weigh 1kg and place it in an extraction tank, add 10 times the amount of 80% ethanol for microwave extraction for 1 hour, extract 3 times, combine the three extracts and let cool, filter, add the filtrate 1.5% medicinal activated carbon is heated and stirred, filtered, the decolorized filtrate is concentrated to a relative density of 1.12, and then passed through a hollow fiber ultrafiltration membrane system with a molecular weight cut-off of 10,000 Daltons, and the operating pressure is adjusted to 0.1MPa to collect the permeate , put the permeate on the pretreated macroporous adsorption resin column, after saturated adsorption, elution with water and 70% ethanol in sequence, collect the 70% ethanol eluate, concentrate by rotary evaporation, and dry to obtain the blue calyx tea Total diterpenoids in vegetables, the content of total diterpenoids is 72%. After HPLC detection, the content of cyanine, cyanine B, cyanine...

Embodiment 3

[0016] Grind the green tea leaves into coarse powder, weigh 2 kg and put it in an extraction tank, add 7 times the amount of 60% ethanol for percolation extraction, until the color of the percolation liquid becomes lighter, collect the extract and let it cool, filter, The filtrate is heated and stirred with 2% medicinal activated carbon, filtered, the decolorized filtrate is concentrated to a relative density of 1.05, and then passed through a hollow fiber ultrafiltration membrane system with a molecular weight cut-off of 3000 Daltons, and the operating pressure is adjusted to 0.3MPa. After passing through the liquid, load the permeated liquid on the pretreated macroporous adsorption resin column. After saturated adsorption, it is eluted with water and 30% ethanol in sequence, and the 30% ethanol eluate is collected, concentrated by rotary evaporation, and dried to obtain the calyx calyx The total diterpenoids of the fragrant tea, the content of the total diterpenoids is 61%. A...

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PUM

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Abstract

The invention discloses a preparation method of rabdosia japonica total diterpene. In the method, total diterpene is extracted from rabdosia japonica by extracting with a solvent and enriching with macroporous resin. The preparation method comprises the following steps of: crushing rabdosia japonica leaves into coarse powder; adding a solvent for extracting; cooling a combined extracting solution and filtering; adding active carbon into the filtrate for decoloring; making the decolored solution pass through an ultrafiltration film of 3,000-10,000 dalton; adsorbing the permeate liquid with pretreated macroporous resin; eluting with an ethanol aqueous solution; and concentrating and drying the obtained eluent to obtain rabdosia japonica total diterpene. The method has the advantages of easiness for operating, low energy consumption, high product quality and the like, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and relates to a preparation method of total diterpenes of S. japonica. Background technique [0002] Blue Calyx Rabdosia japonica (Burm.f.) Hara var.glau-cocalyxi (Maxin) Hara is a plant of the genus Glau-cocalyxi (Maxin) of the Labiatae family, also known as Shansuzi, which is widely distributed in northern my country. , has invigorating the stomach, clearing heat and detoxification, promoting blood circulation, antibacterial and anti-inflammatory and anticancer activities. Indigo calyx also has a dose-related cardioprotective effect. Its chemical composition is mainly diterpenoids and triterpenoids. Shen Xiaodan et al. isolated and obtained compounds such as cyanine A, cyanine B, cyanine C, oleanolic acid, ursolic acid, crabapple aldehyde, and suberin from the tea leaves. Among them, calyxin A, calyxin B, calyxin C, calyxin, calyxin, and calyxin belong to kaurane-type diterpenoid...

Claims

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Application Information

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IPC IPC(8): A61K36/53A61P7/02A61P1/16A61P31/20A61P35/00
Inventor 刘东锋郭琴
Owner NANJING ZELANG MEDICAL TECH
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