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Detection method of haemostasis medicine

A detection method and drug technology, which is applied in the detection of capsules and the detection of hemostatic drugs, can solve the problems of human body and environmental hazards, and the undeveloped TLC identification method, liquid phase detection method, content detection method, etc., to reduce harm and purify drugs Market and order, maintenance of security and effective effect

Active Publication Date: 2012-01-18
XIAN CHIHO PHARMA
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003]In the existing detection method of Zhikang Capsules, the TLC identification method of myrrh, the liquid phase detection method of rhubarb, and the content of chrysophanol have not been formulated The detection method, the gas phase determination method of borneol content, and the thin layer chromatography identification method of Coptis chinensis contain benzene, which is easy to cause harm to human body and environment

Method used

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  • Detection method of haemostasis medicine
  • Detection method of haemostasis medicine
  • Detection method of haemostasis medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 TLC Identification of Myrrh in Zhikang Capsules

[0068] 1. Test conditions: double-slot expansion cylinder (Shanghai Xinyi); silica gel G (for thin-layer chromatography, Qingdao Ocean Chemical Factory); sodium carboxymethyl cellulose (Sinopharm Chemical Reagent Co., Ltd.); self-made thin-layer plate, thickness 0.3mm. The rest of the reagents were analytically pure.

[0069] 2. Test procedure: Take 3g of the contents of Zhikang Capsules (provided by Xi’an Qianhe Pharmaceutical Co., Ltd.), put them in a stoppered Erlenmeyer flask, add 50ml of absolute ethanol, ultrasonicate for 60 minutes, filter, and evaporate the filtrate to dryness. Add 50ml of water to dissolve, filter, extract the filtrate with ether 3 times, 20ml each time, collect and combine the ether solution, evaporate to dryness, add 1ml of petroleum ether (60-90°C) to the residue to dissolve, and use it as the test solution. Take another 0.3 g of myrrh reference medicinal material, and prepare t...

Embodiment 2

[0072] Example 2 TLC Identification of Coptis Rhizoma in Zhikang Capsules

[0073] 1. Test conditions: double-slot expansion cylinder (Shanghai Xinyi); silica gel G (for thin-layer chromatography, Qingdao Ocean Chemical Factory); sodium carboxymethyl cellulose (Sinopharm Chemical Reagent Co., Ltd.); self-made thin-layer plate, thickness 0.3mm. The rest of the reagents were analytically pure.

[0074] 2. Test procedure: take 3 g of the content of Zhikang Capsules (provided by Xi’an Qianhe Pharmaceutical Co., Ltd.), add 20 ml of 1% hydrochloric acid methanol solution, ultrasonically treat for 20 minutes, filter, and concentrate the filtrate to 1 ml as the test sample solution. Take another 0.1 g of Coptis rhizome reference drug, add 5 ml of 1% hydrochloric acid methanol solution, filter, and use the filtrate as the reference drug solution. Then take the negative sample without Coptis rhizome prepared according to the prescription ratio and process, and prepare the negative ...

Embodiment 3

[0075] Example 3 Determination of rhubarb in rhubarb by liquid chromatography

[0076] 1. Detection method

[0077] Determined with reference to high performance liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2010 Edition).

[0078] 1. Chromatographic conditions and system suitability test

[0079] Octadecylsilane bonded silica gel is used as filler; methanol-water (36:64) is used as mobile phase; the detection wavelength is 320nm; the number of theoretical plates should not be less than 2000 based on the calculation of phedin peak.

[0080] 2. Preparation of reference solution

[0081] Take an appropriate amount of rhubarb reference substance, weigh it accurately, add methanol to make a solution containing 10 μg per 1 ml, and obtain it.

[0082] 3. Preparation of the test solution

[0083] Take the contents of Zhikang Capsules (provided by Xi’an Qianhe Pharmaceutical Co., Ltd.), grind finely, take 0.5g, accurately weigh it, put it in a 50ml measuring bott...

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Abstract

The invention provides a detection method of a haemostasis medicine. The haemostasis medicine is composed of rhubarb, Chinese goldthread rhizome, panax notoginseng, angelica archang lica, ass-hide gelatin, dragon bones, common bletilla tuber, myrrh, cuttlebone, Indian madder root and Chinese dragon blood. The detection method comprises steps that: myrrh in Zhikang capsules is identified by using a thin layer chromatography method, Chinese goldthread rhizome in the Zhikang capsules is identified by using the thin layer chromatography method, ponticin and chrysophanol in rhubarb are detected by using a liquid chromatography method, and the borneol content in the Zhikang capsules is detected by using a gas chromatography method. With the method provided by the invention, the quality of the Zhikang capsules can be effectively controlled, such that the quality of the Zhikang capsules reaches standards of stable, controllable, highly efficient and safe. With the method, the feeding requirements and the raw material qualities are strictly controlled, defects of existing technologies are solved, product quality is improved, product curative effect is improved, and product bioavailability is improved, such that medical requirements are satisfied.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a detection method of a hemostatic drug, in particular to a detection method of a capsule (Zhikang capsule) used for hemostasis. Background technique [0002] Zhikang Capsules are composed of fourteen traditional Chinese medicinal materials, including rhubarb, coptis, notoginseng, angelica, donkey-hide gelatin, keel (calcined), baiji, myrrh (made), sea octopus, madder, and dragon's blood, which have synergistic effects. It conforms to the principle of formulating traditional Chinese medicine and achieves the purpose of treating both symptoms and root causes. The preparation absorbs the essence of ancient prescriptions such as "Qilisan", "Xileisan", "Rujinshengjisan", "Tiefansan" and "Daojiansan", and has the functions of "stopping bleeding, promoting tissue repair, anti-inflammatory effect. It has been proved to have remarkable clinical curative effect in the proce...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/02
CPCG01N33/94G01N30/90G01N2500/00G01N30/94
Inventor 胡小虎张琼
Owner XIAN CHIHO PHARMA
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