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Joint vaginitis detection kit

A technology of combined detection and detection reagents, applied in the determination/inspection of microorganisms, material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc. Influence and other issues, to achieve the effect of simple operation, high coincidence rate, high sensitivity and specificity

Inactive Publication Date: 2012-01-18
泰普生物科学(中国)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is simple and easy to implement, but this method is easily affected by human factors (such as the experience of inspectors, the quality of microscopic equipment, sample collection, and the operator's ability to identify odors, etc.), and factors unrelated to BV infection ( Influenced by many factors such as recent sexual intercourse, vaginal douching, menstrual period or postmenopause, it is highly subjective
Moreover, bacterial vaginosis is a gradual process, and the diagnostic criteria of the Amsel method, which is divided into two, limits its detection of BV.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 The preparation of the combined detection kit of the present invention:

[0023] Whatman glass microfiber filter paper 23SL with a pore size of 4.5 mm is installed in the four holes of the hole-type card-like reaction device as a carrier for hydrogen peroxide concentration reagent pads, leukocyte esterase assay reagent pads, sialidase assay reagent pads, and preserved Amino acid aminopeptidase assay reagent pad.

[0024] The making of hydrogen peroxide reagent pad: the pad carrier is soaked in the following soaking liquid (hydrogen peroxide detection reagent) and then dried, the soaking liquid is 8.0g / L N-ethyl-N-(2-hydroxy -3-sulfopropyl)-3-methylaniline sodium salt (TOOS).

[0025] The making of leukocyte esterase detection reagent pad: soak the pad carrier in the following soaking liquid (leukocyte esterase detection reagent), the soaking liquid is: 5-bromo-4-chloro-3-indole acetate 0.5g / L; sucrose 35g / L.

[0026] Preparation of the sialidase detecti...

Embodiment 2

[0030] Embodiment 2 Another embodiment of the combined detection kit of the present invention:

[0031] The making of hydrogen peroxide reagent pad: carry out dry treatment after pad carrier is soaked in following soaking liquid, and described soaking liquid is 15g / L 3,5-dichloro-2-hydroxybenzene sulfonate sodium (DHBS), after that Dry it.

[0032] Preparation of leukocyte esterase detection reagent pad: Soak the pad carrier in the following soaking solution: 2 g / L of 5-bromo-4-chloro-3-indole acetate; 40 g / L of sucrose.

[0033] Preparation of the sialidase detection reagent pad: the pad carrier is soaked in the following soaking solution (sialidase activity detection reagent) of the detection reagent and then dried. The soaking solution is the reagent pad that is then dried. The sialidase activity detection reagent is composed of the following substances: nitrobenzene-N-acetyl-α-sialoside 0.5-5g / L; sucrose: 40g / L; 0.1-1.0% solid purple B solution, followed by Dry processin...

Embodiment 3

[0037] Embodiment 3 Another embodiment of the combined detection kit of the present invention:

[0038] The making of hydrogen peroxide reagent pad: carry out dry treatment after pad carrier is soaked in following soaking liquid, and described soaking liquid is 25g / L 3,5-dichloro-2-hydroxybenzene sulfonate sodium (DHBS), afterwards Dry it.

[0039] Preparation of leukocyte esterase detection reagent pad: soak the pad carrier in the following soaking solution: 2 g / L of 5-bromo-4-chloro-3-indole acetate; 60 g / L of sucrose.

[0040] Preparation of the sialidase detection reagent pad: the pad carrier is soaked in the following soaking liquid of the detection reagent, and then dried. The soaking liquid is: the reagent pad that is then dried. The sialidase activity detection reagent is composed of the following substances: 5 g / L of nitrobenzene-N-acetyl-α-sialoside; 60 g / L of sucrose; 0.5% solid violet B solution, followed by drying.

[0041] Commercially available pH test paper. ...

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PUM

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Abstract

The invention aims to provide a joint vaginitis detection kit which is convenient, quick and sensitive to operate, does not need any high-end instrument and equipment, and has low price. The technical scheme of the invention is to provide the joint vaginitis detection kit comprising a clamp-shaped reaction device with four dry chemical reaction blind holes, diluent and chromogenic reagent; and the four blind holes are respectively provided with a hydrogen peroxide concentration determination test reagent pad, a sialic acid glucoside enzyme activity test reagent pad, a leukocyte esterase activity determination test reagent pad and a vaginal fluid pH test reagent pad. The joint vaginitis detection kit can simultaneously test four indexes, i.e. the pH value of vaginal secretion, hydrogen peroxide, sialic acid glucoside enzyme and leukocyte esterase. Through the four indexes, the micro-ecological environment of vagina is determined, and the joint vaginitis detection kit is for identifying bacterial vaginosis of normal vaginal flora and bacteria groups.

Description

technical field [0001] The invention relates to a combined detection kit for vaginitis, especially for auxiliary diagnosis of female bacterial vaginosis. Background technique [0002] Bacterial vaginosis (BV) refers to a type of bacteriologically manifested as a decrease in the number of normal flora of the reproductive tract (hydrogen peroxide-producing lactobacilli), and is infected by a variety of pathogenic anaerobic bacteria ( It is a disease caused by Gardnerella vaginalis without obvious mucosal inflammation. Bacterial vaginosis is closely related to trichomonas infection, and 80% of trichomonas patients are accompanied by BV infection; it is the main cause of fungal vaginitis, and it is easy to cause salpingitis, pelvic inflammatory disease, urinary system infection, postoperative infection, and Infertility, ectopic pregnancy, and gynecological tumors. BV is prone to occur due to endocrine changes during pregnancy, with an average infection rate of 16-37%. BV durin...

Claims

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Application Information

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IPC IPC(8): C12Q1/28C12Q1/44C12Q1/34C12Q1/04G01N21/80
Inventor 林德增胡守旺姚铭锋谢佐福周晓强阳卫超陈宁崔天星罗娜
Owner 泰普生物科学(中国)有限公司
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