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Ultrasonic-microwave synergic extraction method of chondroitin sulfate

A technology of chondroitin sulfate and ultrasound, applied in the field of ultrasonic-microwave collaborative extraction of chondroitin sulfate

Inactive Publication Date: 2012-01-18
HAINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the extraction of chondroitin sulfate by ultrasonic-microwave co-extraction

Method used

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  • Ultrasonic-microwave synergic extraction method of chondroitin sulfate
  • Ultrasonic-microwave synergic extraction method of chondroitin sulfate
  • Ultrasonic-microwave synergic extraction method of chondroitin sulfate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Cook the leftovers of tilapia (including fish head, fins and fish tails) in a water bath at 80°C for 20 minutes, after cleaning, dry them at 60°C, and after crushing, add volume percent to the leftovers powder Concentration is 99% acetone, the weight of adding acetone is 4 times of the leftover powder weight, after fully stirring, let it stand for 20min to degrease, then pass through a 60 mesh sieve to obtain fish bone powder; accurately weigh 5.000g fish bone powder, and then Add 300g of NaOH solution with a volume percentage concentration of 2% to the fish bone powder; extract the mixture of fish bone powder and NaOH solution in an ultrasonic-microwave collaborative extraction apparatus, and set its parameters as: ultrasonic wave 50w, microwave 200w, extraction time 120s; after the extraction is completed, extract the extract in a 60°C water bath for 30 minutes, filter to obtain 294g of extract; after the extract is naturally cooled to room temperature, adjust the pH o...

Embodiment 2

[0031]Cook the leftovers of tilapia (only fish heads) in a water bath at 70°C for 15 minutes, after cleaning, dry them at 55°C, and after crushing, add 99% vol. Acetone, the weight of adding acetone is 5 times of the leftover powder weight, after fully stirring, let it stand for 30min to degrease, then pass through a 50-mesh sieve to obtain fish bone powder; accurately weigh 4.000g fish bone powder, and then pour it into the fish bone powder Add 280g of NaOH solution with a concentration of 1% by volume; extract the mixture of fishbone powder and NaOH solution in an ultrasonic-microwave cooperative extraction apparatus, and set its parameters as follows: ultrasonic wave 40w, microwave 300w, extraction time 90s; after the extraction is completed , the extract was leached in a 50°C water bath for 20 minutes, filtered to obtain 272g of extract; after the extract was naturally cooled to room temperature, the pH of the extract was adjusted to 5, and then 5.6g of complex protease was...

Embodiment 3

[0033] Cook the leftovers of tilapia (including fish head, fins and tails) in a water bath at 75°C for 18 minutes, after cleaning, dry them at 50°C, and after crushing, add volume percent Concentration is 99% acetone, the weight of adding acetone is 4.4 times of the leftover powder weight, after fully stirring, stand still for 15min to degrease, then pass through a 55 mesh sieve to obtain fish bone powder; accurately weigh 5.000g fish bone powder, and then Add 200g of NaOH solution with a volume percentage concentration of 3% to the fish bone powder; extract the mixture of fish bone powder and NaOH solution in an ultrasonic-microwave cooperative extraction apparatus, and set its parameters as: ultrasonic wave 30w, microwave 60w, extraction time 270s; After the extraction is completed, extract the extract in a 70°C water bath for 40 minutes, filter to obtain 194g of extract; after the extract is naturally cooled to room temperature, adjust the pH of the extract to 7, and then ad...

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Abstract

The invention discloses an ultrasonic-microwave synergic extraction method of chondroitin sulfate, which comprises the following steps: cleaning processing leftovers of tilapia, baking, pulverizing, degreasing, and passing through a 60-mesh screen to obtain fishbone powder; extracting in an ultrasonic-microwave extraction instrument; after the extraction finishes, leaching, filtering, cooling, regulating the pH value, and adding 2% of compound protease to carry out enzymolysis for 2 hours; and after the enzymolysis finishes, inactivating the protease, decolorizing, centrifugalizing, purifyingand concentrating the supernatant with an ultrafiltration apparatus, and precipitating with ethanol to obtain an insoluble substance which is the chondroitin sulfate. The method fully utilizes the cavitation action of ultrasonic vibrations and the high-energy action of microwaves, shortens the extraction time, and obviously enhances the extraction efficiency, thereby greatly lowering the production cost. The invention is simple to operate, and has the advantages of short extraction period, high extraction rate and high product purity.

Description

technical field [0001] The invention relates to a method for extracting chondroitin sulfate, in particular to an ultrasonic-microwave cooperative extraction method for chondroitin sulfate. Background technique [0002] Chondroitin sulfate (CS) is a class of glycosaminoglycans (GAGs), which is based on a disaccharide that combines D-acetyl-D-galactosamine with 1,3 glycosidic bonds and D-glucuronic acid. Macromolecular polysaccharides formed by unit polymerization can be divided into chondroitin sulfate A (CS-A), chondroitin sulfate C (CS-C) and so on according to the position of the sulfate group on N-acetyl-D-galactosamine . Widely present in the connective tissue of animals, it has the functions of preventing vascular sclerosis, lowering blood fat, anticoagulation, antitumor, protecting cornea and moisturizing, etc. It has been widely used in the fields of medicine, biomaterials and cosmetics. [0003] There are many extraction techniques for chondroitin sulfate, mainly i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 段振华成长玉樊洁
Owner HAINAN UNIV
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