Detection kit and detection method of clopidogrel administration related gene subtyping

A genotyping and kit technology, applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of not being able to satisfy a large number of samples, high specificity, genotyping specificity, and limited detection speed

Inactive Publication Date: 2019-03-19
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above scheme only uses real-time fluorescent quantitative PCR technology combined with specific probes, which is still limited in terms of improving genotyping specificity and detection speed, and cannot meet the needs of rapid and highly specific detection of a large number of samples.

Method used

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  • Detection kit and detection method of clopidogrel administration related gene subtyping
  • Detection kit and detection method of clopidogrel administration related gene subtyping
  • Detection kit and detection method of clopidogrel administration related gene subtyping

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] This embodiment provides a nucleic acid amplification reagent, comprising the following concentrations of components:

[0090] High-fidelity DNA polymerase, 550mg / L;

[0091] dNTPs, 100g / L;

[0092] 10×PCR amplification buffer, 10 μL;

[0093] Nano silica, 105g / L;

[0094] Alkylphenol polyoxyethylene ether (APEO), 1mL / L;

[0095] Add double distilled water to 50 μL.

[0096] The above-mentioned nano silicon dioxide is spherical, 30nm.

[0097] The above-mentioned nano silicon dioxide is a commercially available product, such as purchased from Changtai Micro-Nano Chemical Factory in Shouguang City, Shandong Province.

Embodiment 2

[0099] This embodiment provides a nucleic acid amplification reagent, comprising the following concentrations of components:

[0100] Taq enzyme, 100mg / L;

[0101] dNTPs, 200g / L;

[0102] Tris-HCl, 0.5mL / L;

[0103] MgCl 2 , 40g / L;

[0104] KCl, 5g / L;

[0105] (NH 4 ) 2 SO 4 , 40g / L;

[0106] Nano silica, 20g / L;

[0107] Triton X-100, 5mL / L.

[0108] The above-mentioned nano silicon dioxide is spherical, 50nm.

[0109] Above-mentioned nano silicon dioxide, preparation method is as follows: ammoniacal liquor (concentration is 26wt%), water, dehydrated alcohol are placed in A bottle according to volume ratio 10: 0.5: 89.5, tetraethyl orthosilicate (TEOS) and no Water and ethanol are mixed according to the volume ratio of 15:85 and placed in bottle B. Mix the two bottles of solutions on a heating stirrer, stir and preheat to a reaction temperature of 100° C., and then mix them and add them to a three-neck flask with a thermal constant temperature heating magnetic stir...

Embodiment 3

[0111] This embodiment provides a nucleic acid amplification reagent, comprising the following concentrations of components:

[0112] Taq enzyme, 500mg / L;

[0113] dNTPs, 50g / L;

[0114] Tris-HCl, 2mL / L;

[0115] MgCl 2 , 10g / L;

[0116] KCl, 20g / L;

[0117] (NH 4 ) 2 SO 4 , 5g / L;

[0118] Nano silica, 100g / L;

[0119] Triton X-100, 1 mL / L.

[0120] The above-mentioned nano silicon dioxide is spherical, 500nm.

[0121] Above-mentioned nano silicon dioxide, preparation method is as follows: ammoniacal liquor (concentration is 28wt%), water, dehydrated alcohol are placed in bottle A according to volume ratio 30: 2: 68, tetraethyl orthosilicate (TEOS) and no Water and ethanol are mixed according to the volume ratio of 30:70 and placed in bottle B. After mixing the two bottles of solutions, they were stirred on a heating stirrer and preheated to a reaction temperature of 120° C., and then mixed and added to a three-necked flask with a thermal constant temperature heati...

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Abstract

The invention belongs to the technical field of gene amplification detection, and particularly relates to application of nano silicon dioxide in gene amplification detection, a nucleic acid amplification reagent utilizing nano silicon dioxide and a detection kit and a detection method of individualized administration related gene subtyping. It is found for the first time that reaction efficiency and reaction specificity of gene amplification can be improved remarkably by using nano silicon dioxide in gene amplification detection, and the problem that gene amplification is low in detection rateand specificity in the prior art and the problem that quick and highly specific detection of samples in a large amount cannot be realized due to low specificity and slow detection speed of gene typing are solved.

Description

technical field [0001] The invention belongs to the technical field of gene amplification detection, and specifically relates to the use of nano silicon dioxide in gene amplification detection, nucleic acid amplification reagents using nano silicon dioxide, and detection kits for genotyping related to clopidogrel medication ,Detection method. Background technique [0002] Precision medicine is one of the important development trends of modern medicine. On the basis of the knowledge system of the molecular mechanism of the disease obtained through large sample research, based on biomedicine, and according to the specificity of individual patients in various aspects such as genotype, phenotype, environment and lifestyle, formulate precise prevention and precision medicines suitable for individuals. Diagnosis and precision treatment options. Genes are the basis for the basic structure and performance of life. From a genetic perspective, individual differences come from singl...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858
CPCC12Q1/6858C12Q1/6883C12Q2600/106C12Q2600/156
Inventor 周连群李金泽张芷齐李传宇姚佳张威郭振
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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