Detection kit and detection method of clopidogrel administration related gene subtyping
A genotyping and kit technology, applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of not being able to satisfy a large number of samples, high specificity, genotyping specificity, and limited detection speed
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Embodiment 1
[0089] This embodiment provides a nucleic acid amplification reagent, comprising the following concentrations of components:
[0090] High-fidelity DNA polymerase, 550mg / L;
[0091] dNTPs, 100g / L;
[0092] 10×PCR amplification buffer, 10 μL;
[0093] Nano silica, 105g / L;
[0094] Alkylphenol polyoxyethylene ether (APEO), 1mL / L;
[0095] Add double distilled water to 50 μL.
[0096] The above-mentioned nano silicon dioxide is spherical, 30nm.
[0097] The above-mentioned nano silicon dioxide is a commercially available product, such as purchased from Changtai Micro-Nano Chemical Factory in Shouguang City, Shandong Province.
Embodiment 2
[0099] This embodiment provides a nucleic acid amplification reagent, comprising the following concentrations of components:
[0100] Taq enzyme, 100mg / L;
[0101] dNTPs, 200g / L;
[0102] Tris-HCl, 0.5mL / L;
[0103] MgCl 2 , 40g / L;
[0104] KCl, 5g / L;
[0105] (NH 4 ) 2 SO 4 , 40g / L;
[0106] Nano silica, 20g / L;
[0107] Triton X-100, 5mL / L.
[0108] The above-mentioned nano silicon dioxide is spherical, 50nm.
[0109] Above-mentioned nano silicon dioxide, preparation method is as follows: ammoniacal liquor (concentration is 26wt%), water, dehydrated alcohol are placed in A bottle according to volume ratio 10: 0.5: 89.5, tetraethyl orthosilicate (TEOS) and no Water and ethanol are mixed according to the volume ratio of 15:85 and placed in bottle B. Mix the two bottles of solutions on a heating stirrer, stir and preheat to a reaction temperature of 100° C., and then mix them and add them to a three-neck flask with a thermal constant temperature heating magnetic stir...
Embodiment 3
[0111] This embodiment provides a nucleic acid amplification reagent, comprising the following concentrations of components:
[0112] Taq enzyme, 500mg / L;
[0113] dNTPs, 50g / L;
[0114] Tris-HCl, 2mL / L;
[0115] MgCl 2 , 10g / L;
[0116] KCl, 20g / L;
[0117] (NH 4 ) 2 SO 4 , 5g / L;
[0118] Nano silica, 100g / L;
[0119] Triton X-100, 1 mL / L.
[0120] The above-mentioned nano silicon dioxide is spherical, 500nm.
[0121] Above-mentioned nano silicon dioxide, preparation method is as follows: ammoniacal liquor (concentration is 28wt%), water, dehydrated alcohol are placed in bottle A according to volume ratio 30: 2: 68, tetraethyl orthosilicate (TEOS) and no Water and ethanol are mixed according to the volume ratio of 30:70 and placed in bottle B. After mixing the two bottles of solutions, they were stirred on a heating stirrer and preheated to a reaction temperature of 120° C., and then mixed and added to a three-necked flask with a thermal constant temperature heati...
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