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Anti-inflammatory pharmaceutical composition comprising extracts from mulberry and honeysuckle

A technology for extracts and compositions, applied in the field of anti-inflammatory pharmaceutical compositions comprising extracts from paper mulberry and honeysuckle, can solve problems such as side effects, inability to fundamentally treat COPD, and achieve less side effects or adverse effects, wide-ranging Effectiveness of therapeutic range and synergy, good anti-inflammatory and analgesic effects

Inactive Publication Date: 2011-12-21
PHARMAKING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, they have side effects and cannot fundamentally treat COPD, chronic inflammation such as atopic diseases, and allergic diseases

Method used

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  • Anti-inflammatory pharmaceutical composition comprising extracts from mulberry and honeysuckle
  • Anti-inflammatory pharmaceutical composition comprising extracts from mulberry and honeysuckle
  • Anti-inflammatory pharmaceutical composition comprising extracts from mulberry and honeysuckle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 prepares extract by mulberry and honeysuckle

[0042] Paper mulberry is collected from the southern region of Korea (near Andong). The root bark of the mulberry tree was dried, and the dried root bark was finely cut and subjected to an extraction process using 100% ethanol. The ethanol extract was dried under vacuum and the final extract was dispersed in water and fractionated with ethyl acetate, then the ethyl acetate fraction was dried. The dried ethyl acetate fraction (EBP) was used for experiments. Papyriflavonol A and schalcone A were isolated from EBP according to the method described in Son et al. (2001).

[0043] Honeysuckle was obtained from the oriental medicine market. The dried honeysuckle was finely chopped and then subjected to an extraction process using 70% ethanol. The ethanol extract was dried under vacuum and the dried ethanol extract (ELJ) was used in the experiment. Loganin and Sweroside were isolated from this ethanol extract acco...

Embodiment 2

[0046] Example 2 Rat Basophilic Leukemia-1 (RBL-1) Cell Culture and Leukotriene (LT) Measurement

[0047] RBL-1 cells obtained from the American Type Culture Collection (American Type Culture Collection) (ATCC, Rocksville) were grown in RPMI 1640 supplemented with 10% FBS, 2 mM glutamine, and 1% antibiotics at 5% CO 2 and incubated at 37°C. Cells were placed in 96-well plates for 2 hours and pre-incubated with test substances for 10 minutes. Test substances were dissolved in DMSO and diluted with appropriate concentrations of serum-free DMEM. The final concentration of DMSO was adjusted to 0.1% (v / v). Cell viability was verified by MTT assay according to the method published in Mossman (1983). To activate 5-LOX, A-23187 (ionophore, 3 μM) was added to the cells and the cells were incubated for 15 minutes according to a slightly modified method disclosed in Tries et al. (2002). The used medium was collected and the concentration of cysteinyl leukotrienes (LTC4 / D4 / E4) produce...

Embodiment 3

[0055] Example 3 produces PGE in RAW 264.7 cells 2 Impact

[0056] This experiment was performed to determine the effect of extracts from mulberry and honeysuckle on the production of PGE 2 Effect of PGE 2 mediator of inflammatory reactions.

[0057] RAW 264.7 cells obtained from the American Type Culture Collection (ATCC, Rocksville) were supplemented with 10% FBS and 1% antibiotics (100 U / ml penicillin and 100 μg / ml streptomycin )) in DMEM at 5% CO 2 and incubated at 37°C. The cells were activated with lipopolysaccharide (LPS) according to the method disclosed in Chi et al. (2001). Cells were loaded into 96-well plates (2x10 5 cells / well) and pre-incubated for 2 hours, then the test substance and LPS (1 μg / ml) were added thereto, and the resulting medium was incubated for 24 hours, unless otherwise specified. The concentration of PGE2 in the medium was measured by using a PGE2 ELISA kit (Cayman Chem. Co.) according to the method suggested by the manufacturer.

[0058...

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PUM

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Abstract

The present invention relates to a pharmaceutical composition for alleviating or treating inflammations and / or pains, the pharmaceutical composition comprises extracts from Broussonetia papyrifera and Lonicera japonica as an effective component. The present invention is based on a finding that when a prenylated flavonoid-enriched extract from Broussonetia papyrifera and an ethanol extract from Lonicera japonica are administered in combination, excellent and synergetic anti-inflammatory and analgesic effects are obtained in in vitro and in vivo tests, compared to when the extracts are separately administered.

Description

technical field [0001] The present invention relates to a pharmaceutical composition for preventing, alleviating, or treating inflammation or pain, the pharmaceutical composition comprising extracts from Broussonetia papyrifera and Lonicera japonica as active ingredients. Background technique [0002] Inflammation is a preventive response that occurs in the body when biological tissue is damaged, and can cause congestion, edema, fever, and pain in a part of the body in response to trauma, burns, or bacterial infection. Inflammatory signals are produced through cyclooxygenase (COX) pathway and lipoxygenase (LOX) pathway, and prostaglandins, leukotrienes, thromboxanes, etc. are produced through these pathways. Once the inflammatory signal is transferred to the target site in vivo, various biological changes occur. For example, blood vessels that are subject to inflammation close to the target site dilate, thereby allowing more blood to be supplied to the vessel so that blood ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/60A61K36/355A61P29/00A61P11/06
CPCA61K36/355A61K36/60A61P1/00A61P1/02A61P1/04A61P1/06A61P1/16A61P9/00A61P11/00A61P11/02A61P11/06A61P13/00A61P17/00A61P17/06A61P19/02A61P21/00A61P25/04A61P29/00A61P37/00A61P37/06A61K2300/00
Inventor 金淳培金光淳金完培郭义宗方诚惠金显杓孙建镐
Owner PHARMAKING
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