A kind of stage-by-stage solid culture method of Beauveria bassiana

A technology for solid culture of Beauveria bassiana, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems that hinder the production and application of Beauveria bassiana insecticides, control effects, and unstable product quality , high temperature and humidity requirements, to overcome the uncontrollable humidity, to meet the needs of mycelium growth, and to achieve the effect of low production cost

Inactive Publication Date: 2011-12-21
FUJIAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the solid-state fermentation stage, most manufacturers in my country currently adopt open production modes such as shallow trays and screen windows. The production process has high requirements for temperature and humidity, long fermentation cycles, easy infection of miscellaneous bacteria, and unstable product quality. Problems hinder the production, application and control effect of Beauveria bassiana insecticides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. The cultivation of liquid strains comprises the following steps:

[0039] 1.1 Strains: Beauveria bassiana BbCeFZ01 strain (Fujian Forestry Science and Technology, 2006, 33(2): 129-131.), preserved by the Forest Protection Institute of Fujian Academy of Forestry Sciences, is a known strain.

[0040] 1.2 Slant strains: Subculture and activate the Beauveria bassiana strains in test tube slant medium at 25°C in advance, and use them when the surface of the slant is covered with spores. The slant medium is SDAY medium.

[0041] 1.3 Liquid medium: Prepare 2L of SDY medium, put it into 500ml Erlenmeyer flasks, each containing 200ml of medium, steam sterilize at 121°C for 30min, cool down and set aside.

[0042] 1.4 Inoculation: In a sterile operating table, pick a small amount of spores and bacterial lawns from the strain slope and insert them into the above liquid medium.

[0043] 1.5 Shaker culture: Shake culture in a constant temperature shaking incubator at 25±1°C for...

Embodiment 2

[0053] 1. The cultivation of liquid strains comprises the following steps:

[0054] 1.1 Strains: Beauveria bassiana BbSMYXTR03 strain (Fujian Forestry Science and Technology, 2011, 38 (2): 10-13), preserved by the Forest Protection Institute of Fujian Academy of Forestry Sciences, is a known strain.

[0055] 1.2 Incline strains: the same as in Example 1.

[0056] 1.3 Liquid medium: Prepare a total of 4 L as in Example 1.

[0057] 1.4 Inoculation: Same as in Example 1.

[0058] 1.5 Cultivation on a shaker: Same as in Example 1, except that the culture time on a shaker is 60 h.

[0059] 2 Solid culture, including the following steps:

[0060] 2.1 Formula and preparation of solid compost:

[0061] The formula of solid compost for Beauveria bassiana: 10.7kg of wheat bran, 3.5kg of chaff, and 12.4L of water.

[0062] Preparation method: with embodiment one.

[0063] 2.2 Inoculation: Same as Example 1, except that the inoculation volume is 15%.

[0064] 2.3 Mycelia growth sta...

Embodiment 3

[0068] 1. The cultivation of liquid strains comprises the following steps:

[0069] 1.1 Strains: Beauveria bassiana B08 (Chinese Forest Diseases and Pests, 2008, 27(2): 1-3,18.) strain, preserved by the Forest Protection Institute of Fujian Academy of Forestry Sciences, is a well-known strain.

[0070] 1.2 Incline strains: the same as in Example 1.

[0071] 1.3 Liquid medium: Prepare a total of 8 L as in Example 1.

[0072] 1.4 Inoculation: Same as in Example 1.

[0073] 1.5 Cultivation on a shaker: Same as in Example 1, except that the culture time on a shaker is 60 h.

[0074] 2 Solid culture, including the following steps:

[0075] 2.1 Formula and preparation of solid compost:

[0076] Beauveria bassiana solid culture material formula: wheat bran 20kg, bagasse 5kg, water 25L.

[0077] Preparation method: with embodiment one.

[0078] 2.2 Inoculation: Same as Example 1.

[0079] 2.3 Mycelia growth stage: Same as Example 1.

[0080] 2.4 Sporulation stage: the same as ...

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Abstract

The invention provides a method for carrying out solid culturing on beauveria bassiana by stages, which is characterized in that the beauveria bassiana solid culturing process is divided into two stages, i.e. a mycelium growing stage and a sporulating stage; in the mycelium growing stage, a closed fermenting mode is adopted and a liquid strain is inoculated with a solid culture material in a closed container to carry out culturing; and in the sporulating stage, the solid culture material on which mycelia full grow is taken out in a buckled mode and an open fermenting mode is adopted. The method for carrying out solid culture on the bassiana, which is provided by the invention, has simple process and does not need complex equipment. The fermenting period of the method is shortened to 6 to 7 d from 10 to 14 days in the prior art. The production efficiency is greatly improved. Meanwhile, the requirement on the environment humidity is low. The probability of the mixed fungi pollution is obviously reduced. The production cost is low. The method is easy to control and has high success rate. The product quality is stable. The method can be applied to laboratory production, can be used asthe preferred scheme of upgrading of technology of masses of small and medium bassiana manufacturers and has obvious social and economic benefits.

Description

technical field [0001] The invention relates to a solid culture method for fungi, in particular to a Beauveria bassiana Beauveria bassiana (hereinafter referred to as Beauveria bassiana) solid culture method, which belongs to the field of biological fermentation methods. Background technique [0002] Beauveria bassiana is a kind of entomopathogenic fungi with a wide range of hosts for agricultural and forestry pests and strong pathogenicity. It plays a pivotal role in the research and application of pest biological control. Because Beauveria bassiana is harmless to the environment and vertebrates, easy to cultivate, wide insecticidal spectrum, and strong pathogenicity, it is currently the most widely used fungal insecticide, and has achieved great success in the biological control of various agricultural and forestry pests. Obvious results have been achieved. [0003] At present, the production of Beauveria bassiana at home and abroad mainly adopts the liquid-solid ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 何学友蔡守平黄金水熊瑜杨希
Owner FUJIAN ACAD OF FORESTRY
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