Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Microbial inoculum for high-efficiency fermenting bed and preparation method thereof

The technology of a microbial inoculum and a composite microbial inoculum is applied in the field of microbial inoculum for fermentation bed and its preparation, which can solve the problems of large air pollution, environmental pollution, land occupation, etc., and achieves reduction of pollution, improvement of pork quality, and stable quality. Effect

Inactive Publication Date: 2013-05-22
HUNAN INST OF MICROBIOLOGY
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to statistics, the total amount of solid waste produced by animal husbandry in the country is about 3 billion tons every year. In Changsha City alone, about 80 million tons of livestock and poultry wastewater and more than 1.2 million tons of solid manure are discharged every year. Most farms only pass through biogas. After the pool is simply treated, it is directly discharged, causing a lot of pollution to the environment
[0003] At present, most of the traditional pig manure treatment technologies use composting fermentation, biogas fermentation, aerobic fermentation and drying technology, etc. Composting and fermentation treatment of manure takes up land, causing great air pollution to the environment, and biogas fermentation has seasonal limitations. The cost of aerobic fermentation and drying technology is relatively high, and there are certain limitations in practical application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial inoculum for high-efficiency fermenting bed and preparation method thereof
  • Microbial inoculum for high-efficiency fermenting bed and preparation method thereof
  • Microbial inoculum for high-efficiency fermenting bed and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: preparation of composite bacterial agent

[0051] 1. Preparation of Bacillus subtilis fermentation product

[0052] Activation of slant strains: Store the slant strains in the refrigerator, aseptically inoculate them into fresh test tube slant medium, and culture at a constant temperature of 37°C overnight to make fresh test tube slant strains. Incline medium: peptone 1%, NaCl 0.5%, yeast extract 0.5%, agar 1.5%-2.0%, the rest is water, pH 7.2-7.4.

[0053] Shake flask seed culture: Take a ring of activated fresh slant strains and inoculate it into the shake flask seed liquid medium by aseptic operation, the filling volume is 100mL / 500mL, the rotation speed is 180-200rpm, and the temperature is 34-37℃ for 22-24h Finally, heat treatment in a water bath at 80°C for 10 minutes. The culture medium of the described shaking flask seeds does not add agar in the slant culture medium.

[0054] Fermentation tank culture: The medium loading in the fermentation tan...

Embodiment 2

[0068] Embodiment 2: Utilize the composite bacterial agent of the present invention to prepare the pig raising effect of fermentation bed

[0069] 1. Fermentation bed preparation

[0070] The fermented mattress material is composed of sawdust and rice husk in a weight ratio of 1:1, or is composed of sawdust, chaff and crop straw in a weight ratio of 5:3:2. Sawdust, chaff, and crop stalks play the roles of adsorbing bacteria, retaining moisture, loosening and ventilating, and providing C and N sources for functional microorganisms. Before making the fermentation bed, it is best to expose sawdust, rice husk, straw, etc. to the sun for 1 to 2 days, and the pens should be thoroughly disinfected.

[0071] Fermented mattress materials should have no mildew, no corruption, no foul smell or other peculiar smells. The particle size of the material is 2-10mm, the carbon-nitrogen ratio is 40-100:1, the number of fecal coliform bacteria is ≤100cfu / g, the mortality rate of roundworm eggs...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a microbial inoculum for a high-efficiency fermenting bed and a preparation method thereof. The microbial inoculum is prepared by thoroughly and evenly mixing 30-40% of Bacillus subtilis leavening powder, 30-40% of Bacillus licheniformis leavening powder and 20-30% of Candida utilis leavening powder. The microbial inoculum can effectively promote in-situ degradation of livestock and poultry excrement, obviously reduce the generation of ammonia and other putrilages in the livestock and poultry excrement, and enhance the quality of pork.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a microbial bacterial agent for a fermentation bed and a preparation method thereof. Background technique [0002] In recent years, with the rapid development of large-scale and intensive pig farming in my country, the pollution of livestock and poultry manure and sewage to the environment has become increasingly serious. According to statistics, the total amount of solid waste produced by animal husbandry in the country is about 3 billion tons every year. In Changsha City alone, about 80 million tons of livestock and poultry wastewater and more than 1.2 million tons of solid manure are discharged every year. Most farms only pass through biogas. After the pool is simply treated, it is directly discharged, which causes a lot of pollution to the environment. [0003] At present, the traditional pig manure treatment technology mostly adopts composting fermentation, biogas ferm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20C12N1/16C12R1/10C12R1/125C12R1/72
Inventor 尹红梅贺月林张德元吴迎奔陈薇许丽娟王震周映华吴胜莲
Owner HUNAN INST OF MICROBIOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com