A concentration gradient diffusion sampling device for an online detection system of a bioreactor

Abstract

The invention relates to a sampling device suitable for bioreactors such as microbial fermentation and cell culture, in particular to a concentration gradient diffusion sampling device, which is based on the concentration difference caused by the concentration difference of each component in the liquid in the solution on both sides of the separation membrane. The principle is to form a concentration gradient distribution of components (measured samples) in the sampling pipeline, and the flux or rate of diffusion follows Fick's law. Using this device to sample the samples in the bioreactor has the advantages of effectively preventing sampling pollution and good safety; there is no filtration process of the solution, the membrane pores should not be blocked, and the stability of continuous sampling is high; the sample components are in the sampling pipeline A gradient distribution is formed, which can be measured without cumbersome dilution, making the online detection system simpler, with faster measurement speed and wider linear range, and is especially suitable for on-line detection of various flow injection analysis systems.

Description

technical field [0001] The invention relates to a sampling device suitable for on-line detection systems of bioreactors such as microbial fermentation and cell culture. Background technique [0002] A bioreactor is a device system that uses the biological functions of enzymes or organisms (such as microorganisms) to perform biochemical reactions in vitro. It is a biological function simulator (container), such as a fermenter, enzyme or cell reactor, etc. , in food and fermentation (such as wine, amino acid, organic acid), medicine (antibiotics, interferon), environment (biodegradation), bioenergy (cellulosic ethanol, oil-producing microalgae, biogas) and other production and scientific research fields application. [0003] In order to maintain the high-efficiency operation of the bioreactor, that is, to obtain the maximum biotransformation rate and the lowest cost, it is necessary to control the biological process in the reactor, and the basis of the control is to detect va...

AI Technical Summary

Problems solved by technology

The disadvantage of the sampling device composed of pipeline valves is that what is taken out is the original sample in the reactor, containing bacteria, raw material slag, etc., which often need to be filtered before entering the instrument for measurement; Sterilization devices, such as steam, ultraviolet lamps, etc.

For separation membrane filtration sampling, because the pore size of the membrane prevents the passage of bacteria and avoids external pollution, the disadvantage is that impurities in the liquid can easily cause blockage of the separation membrane mesh, poor filtration stability, and need to be cleaned or replaced frequently

In addition, the common disadvantages of these two types of sampling methods are: due to the large changes in the sample concentration in the reactor, the samples taken out need to be diluted in different proportions before they can be measured, which requires the configuration of complex dilution devices, which increases the sampling cost. The cost of the system is also likely to cause errors in the measurement results (dilution errors)

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