Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of ointment for treating psoriasis based on anti-tnf-alpha monoclonal antibody

A monoclonal antibody, psoriasis technology, applied in the direction of antibody, ointment delivery, skin diseases, etc., can solve problems such as influence and adverse reactions, and achieve the effect of reducing production cost, good therapeutic effect, and simplifying complex operation links

Active Publication Date: 2011-12-14
赵宁
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the anti-TNF-α method is more effective and safer than the traditional treatment for many diseases related to the overexpression of TNF-α, the TNF-α monoclonal antibody may affect the cytokine network in the human body after intravenous injection. cause adverse reactions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of ointment for treating psoriasis based on anti-tnf-alpha monoclonal antibody
  • A kind of ointment for treating psoriasis based on anti-tnf-alpha monoclonal antibody
  • A kind of ointment for treating psoriasis based on anti-tnf-alpha monoclonal antibody

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0044] 3. Preparation of anti-rhTNF-α monoclonal antibody

[0045] Select female Balb / c mice aged 8-10 weeks, and inject 0.5 ml of liquid paraffin into each intraperitoneal injection. One week later, 1×10 anti-rhTNF-α hybridoma cells were injected intraperitoneally. 6 After ascites was produced in the mouse peritoneal cavity (about 8-12 days), the ascites was taken from the mouse peritoneal cavity, and then centrifuged at 3000 rpm for 20 minutes to separate the ascites, and stored at -80°C for future use.

[0046] 4. Purification process of anti-human TNF-α monoclonal antibody

[0047] ①Principle

[0048] Q-SePharose Fast Flow is a strong anion exchange medium with the characteristics of fast flow rate and high capacity. The PI range of mouse IgG is pH 7.0-8.5, while the PI range of albumin and transferrin is pH 4.7-5.0, so Q-SePharose Fast Flow tends to bind non-targeted substances in ascites in a neutral pH environment. protein. When eluted with a salt solution gradient...

Embodiment 1

[0079] Formula (100kg preparation dosage):

[0080] Ammonium Acryloyldimethyltaurate / VP Copolymer (AVC) 2kg

[0081] Polyethylene glycol PEG 4000 5kg

[0082] Glycerol 5kg

[0083] Preservative (Kaison) 0.03kg

[0084] Anti-TNF-α monoclonal antibody (2.5mg / ml) 400ml (0.4kg)

[0085] Water 87.57kg

Embodiment 2

[0087] Formula (100kg preparation dosage):

[0088] Ammonium Acryloyldimethyltaurate / VP Copolymer (AVC) 1.5kg

[0089] PEG 4000 4kg

[0090] Glycerol 4kg

[0091] Preservative (Kaison) 0.02kg

[0092] Anti-TNF-α monoclonal antibody (2.0mg / ml) 500ml (0.5kg)

[0093] Water 89.98kg

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an ointment based on an anti-TNF-alpha monoclonal antibody used for treating psoriasis, and the ointment comprises the following ingredients by weight: 0.33-0.5 part of anti-TNF-alpha monoclonal antibody, 1.5-2.5 parts of acryloyl dimethyl taurine ammonium / VP copolymer (AVC), 4-6 parts of polyethylene glycol4000, 4-6 parts of glycerol, 0.02-0.04 part of preservative and 85.127-89.98 parts of water. The ointment based on anti-TNF-alpha monoclonal antibody used for treating psoriasis enables the therapeutic effect without adverse reaction caused by using the anti-TNF-alpha antibody by whole body.

Description

technical field [0001] The invention relates to an ointment for treating psoriasis based on anti-TNF-α monoclonal antibody. Background technique [0002] Tumor necrosis factor (TNF-α) is a cytokine with complex biological activities, mainly produced by macrophages and T cells. In addition to killing tumor cells in vivo or in vitro, this substance also has multiple functions such as antiviral infection, activation of macrophages and polymorphonuclear granulocytes, promotion of expression of histocompatibility antigens, and regulation of the body's immune function. However, when TNF-α is overexpressed, it together with other inflammatory factors will produce various pathological damages, such as autoimmune diseases, infectious diseases, dyscrasias and so on. Therefore, neutralizing the excess TNF-α in the body can provide an effective way for the treatment of the above diseases. Current TNF-α antagonist drugs include Infliximab (Humiral), Etanercept (Enbel), Adalimumab (Remi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K39/395A61P17/06
Inventor 赵宁刘节张英起
Owner 赵宁
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com