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process blood

A blood and blood cell technology, applied in the field of white blood cell separation, can solve the problems of waste of materials, manpower and cost, time-consuming, loss of lymphocytes, etc.

Active Publication Date: 2015-09-09
THERAKOS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These current discontinuous processes are time consuming, waste materials, manpower and cost (J.Gryn et al., Journal of Hematotherapy & Stem Cell Research 11 (2002), 719-730; K.R.Meehan et al., Journal of Hematotherapy & Stem Cell Research 9( 2000), 767-771)
These processes also raise serious concerns such as: (i) safety issues arising from potential microbial infection; and (ii) chain-of-custody issues arising from cell selection and modification safeguards (i.e., ensuring correct cells return to the patient and maintain cell integrity)
[0018] Another drawback of the leukapheresis process is the loss of useful lymphocytes in some patients

Method used

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Examples

Experimental program
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example 1

[0107] Example 1. Collection of monocytes and enrichment of CD4+ T lymphocytes from peripheral blood

[0108] Bags of peripheral blood were prepared to represent pseudo patients. Collect 4 units of ABO-matched whole blood from healthy donors into ACD-A anticoagulant 1-2 days before use. The blood of the plurality of units was leukocyte-depleted by filtration through a Sepacell leukocyte-reducing filter, and pooled into a 2L blood bag. A leukopak buffy coat was added to bring the white blood cell count to a physiological concentration, and the sham patient bag was kept on a rocking platform at room temperature to ensure a homogeneous cell suspension. A 10 mL sample was taken from the sham patient bag and the baseline cell composition was determined by electronic cell counting and automatic differentiation on a Beckman Coulter Act counter, and by using a sample containing CD45-FITC, CD3-PECy7, CD4-APC, CD8-PECy5, CD14 - Flow cytometry of monoclonal antibody panels including ...

example 2

[0127] Example 2. Collection of monocytes from peripheral blood and enrichment of CD8+ cells

[0128] overview

[0129] Figure 12 shown with Figure 7 System 700 is related to improvement of system 1200 . For the sake of brevity, Figure 7 and Figure 12 Like features in the system 1200 retain the same reference numerals (e.g., Figure 7 and Figure 12 Anticoagulant pump 712 in . In addition, unless expressly described below, Figure 12 These like-numbered features in the system 1200 also retain the same configuration. Figure 12 The system 1200 is a blood processing device that involves collection and enrichment (type 2) and contains 3 new bags, 6 new clamps and 2 magnets. Such as Figure 12 As shown, a modification of the standard CellEx process kit was made. A CLINIcell 25 bag 1278 was used instead of the photoactivation chamber. Patients 1200 in Figure 12 Denoted as patient bag #1 1206 in , the patient bag #1 is connected to the return node 756, and the ...

example 3

[0152] Example 3. Collection of monocytes from peripheral blood and enrichment of CD34+ cells

[0153] The collection and enrichment of CD34+ cells can be carried out as described in Examples 1 and 2 using substances that specifically bind to CD34.

[0154] Enrichment of CD34+ cells from collected monocytes

[0155] In one embodiment of the invention, the subject may be mobilized using granulocyte colony stimulating factor (G-CSF). Following completion of standard CellEx monocyte collection, washes were performed with cell enrichment buffer PBS / EDTA (Miltenyi) supplemented with HSA and CD34+ selection beads (Miltenyi) introduced via the needle-free access port of the "collection bag". The monocyte and bead mixture was incubated for 30 minutes while recirculating through the trapping module of the modified CellEx single-use kit and by transferring the cells via a peristaltic pump to a Miltenyi CliniMACS magnet at approximately the manufacturer's recommended flow rate. sys...

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Abstract

Methods (300), devices, and systems of processing blood are describes. The method (300) comprises the steps of: obtaining (312) blood from a patient coupled to a single blood processing device to form a closed loop between the patient and the blood processing device; collection (314) bulk mononuclear blood cells from the blood by leukapheresis using the blood processing device in the closed loop; and enriching (316) concurrently target cells separated from non-target cells in the bulk mononuclear blood cells using the blood processing device in the closed loop.

Description

[0001] Cross-references to related patent applications [0002] This application claims priority to US Provisional Application No. 61 / 140,196, filed December 23, 2008, which is hereby incorporated by reference in its entirety. technical field [0003] The present invention relates generally to methods and apparatus for processing blood, and more particularly to methods and apparatus for leukocyte separation. Background technique [0004] Blood cells are continuously produced during the lifespan of an individual and originate from the most primitive blood cells, so-called hematopoietic stem cells (HSCs). Hematopoietic stem cells are capable of giving rise to hematopoietic progenitor cells (HPCs) and blood cells of different cell types (eg, red blood cells (RBC) and white blood cells or white blood cells (WBC)), and are often found in the bone marrow. More mature blood cell types are found in the blood and lymphoid tissues. Hematopoiesis is the continuous production of blood...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61M1/34
CPCA61M2202/0439A61M1/3496A61M1/38A61M1/3696A61M1/3616A61P35/00A61P35/02A61P7/00A61M2202/0064A61M1/1601A61M1/1605A61M1/1615A61M1/3424A61K39/0008A61M1/0209A61M1/3413A61M1/3693A61M1/3626A61M1/34C12M1/10A61M1/362A61M1/3672A61M1/3689B03C1/015B04B5/10B04B11/02
Inventor J·L·麦菲尔森D·佩里特K·亨里奇森G·P·西蒙兹S·彭德P·王
Owner THERAKOS INC
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