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Application of Adrenergic Receptor Blocking Drugs in Antagonizing Cardiotoxicity of Jellyfish Toxin

A receptor blocking, epinephrine technology, used in the field of medicine

Inactive Publication Date: 2011-11-30
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there has been no report of any type of adrenoceptor blocker being used to antagonize the cardiotoxicity of jellyfish toxin

Method used

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  • Application of Adrenergic Receptor Blocking Drugs in Antagonizing Cardiotoxicity of Jellyfish Toxin
  • Application of Adrenergic Receptor Blocking Drugs in Antagonizing Cardiotoxicity of Jellyfish Toxin
  • Application of Adrenergic Receptor Blocking Drugs in Antagonizing Cardiotoxicity of Jellyfish Toxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1. prepare jellyfish crude poisonous extract

[0065] Cyanea capillata was collected from Sanmen Bay, Zhejiang Province in August 2010. Take 10g of tentacles, add 10ml of 3.34% self-prepared seawater in equal volume, autolyze for 4 days, and stir twice a day with a magnetic stirrer. 10 minutes each time. Filter 3 times with a 100-mesh cell sieve, centrifuge the filtrate 3 times at 10,000×g for 15 min each time, and collect the supernatant, which is the jellyfish crude venom extract (TOE, tentacle-only extract). The protein concentration was determined to be 1.2 mg / ml by Bradford method. Self-made seawater formula: NaCl 28g, MgCl 2 ·6H 2 O 5g, KCl 0.8g, CaCl 2 1.033g, add water to 1000ml.

[0066] All operations were carried out under ice water or 4°C. Dialyze with 0.01mol / L, pH 7.4 PBS for 8h before use.

Embodiment 2

[0067] Example 2. The non-selective beta-receptor blocking drug propranolol antagonizes the cardiotoxicity of jellyfish toxin at the three levels of neonatal rat cardiomyocytes, isolated heart and whole animal

[0068] The experimental propranolol raw material was purchased from Shanghai Hualan Chemical Technology Co., Ltd., and it was a white powdery solid.

[0069] 2.1 At the level of neonatal rat cardiomyocytes, propranolol can significantly antagonize the toxicity of jellyfish toxin cardiomyocytes

[0070] Take 15 newborn SD suckling mice, open their chests and take their hearts under aseptic conditions, and immediately use ice-cold D-hanks solution (NaCl: 8.0g; KCl: 0.4g; KH 2 PO 4 : 0.06g; NaHCO 3 : 0.35g; Na 2 HPO 2 12H 2 O: 0.06g, add triple distilled water to 1000ml, filter through a 0.22μm filter) to wash off residual blood, cut into 1mm 3 Fragments of large and small sizes were digested with type II collagenase and Percoll density gradient centrifugation to ob...

Embodiment 3

[0079] Example 3. At the cardiomyocyte level of suckling rats, the α and β receptor blocking drug labetalol antagonizes the cytotoxicity experiment of jellyfish toxin cardiomyocytes

[0080] Labetalol API used in the experiment was purchased from Shanghai Hualan Chemical Technology Co., Ltd., and it was a white powdery solid.

[0081] 3.1 Labetalol has no obvious antagonistic effect on jellyfish toxin at the level of neonatal rat cardiomyocytes

[0082] The concentration of labetalol was 20, 50, 100, 200 μM, and the experimental method was as in Example 2.1. The results showed that labetalol had no obvious antagonistic effect on jellyfish toxin at the cardiomyocyte level of suckling rats (see Table 1, Figure 1-2 ).

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Abstract

The invention relates to the technical field of medicine. Jellyfish stings are the most common marine injury worldwide, and acute circulatory failure caused by cardiovascular toxic components in jellyfish toxins is considered to be the main cause of death from jellyfish stings. The present invention also provides the application of different types of adrenergic receptor blocking drugs in the preparation of jellyfish toxin cardiotoxic antagonist drugs, and the different types of adrenergic receptor blocking drugs refer to α receptor blocking drugs, β receptor blocking drugs And α, β receptor blockers, especially non-selective α receptor blockers, non-selective β receptor blockers, selective long-acting β1 receptor blockers, selective short-acting β1 receptor blockers body blockers. The present invention finds that different types of adrenergic receptor blocking drugs can effectively antagonize the cardiotoxicity of jellyfish toxins at the three levels of cultured suckling rat cardiomyocytes, isolated hearts and whole animals, and can be developed as a preventive or therapeutic drug for jellyfish stings .

Description

technical field [0001] The present invention relates to the technical field of medicine, specifically referring to the ability of different types of adrenergic receptor blocking drugs to antagonize the cardiotoxicity of jellyfish toxins, and further, the application of different types of adrenergic receptor blocking drugs in the preparation of drugs for preventing or treating jellyfish stings . Background technique [0002] Jellyfish stings are the most common injury caused by marine organisms in the world. Jellyfish stings can cause symptoms such as local severe pain, inflammation, and necrosis. In severe cases, muscle spasms, respiratory and circulatory depression, and even shock and death may occur. Jellyfish toxin is a class of peptide toxins with novel structure and strong toxicity. It has various biological activities such as cardiovascular, hemolysis, nerve, and respiratory. Among them, acute circulatory failure caused by cardiovascular toxic components is considered ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/138A61K31/165A61K31/216A61K31/417A61P39/02
Inventor 肖良王蓓蕾张黎明王倩倩贺茜聂菲张林陆佳王涛温小娟张博
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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