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A kind of preparation method of colistin sulfate premix

A technology of colistin sulfate and colistin, which is applied in the direction of medical preparations of non-active ingredients, antibacterial drugs, and pharmaceutical formulations, can solve the problems of long separation steps, environmental pollution, and environmental pollution, and achieve low cost, Strong specificity and less pollution effect

Active Publication Date: 2011-11-30
浙江拜克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using ion exchange method, this method has long separation steps, high energy consumption, and brings a lot of waste water, which seriously pollutes the environment
The invention with the application number 200510122187.3 published on July 12, 2006 discloses a method for preparing polymyxin E by foam separation. Solvent is used for foaming, which pollutes the environment and costs a lot

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Pretreatment of fermentation broth

[0025] Adjust the pH value of the fermented liquid to 3.0 with sulfuric acid, add 3.5%wt diatomite filter aid while stirring, and filter through the plate and frame until no filtrate drips out to obtain the plate and frame filtrate. The plate and frame filtration yield was 96.5%.

[0026] (2) Concentration under reduced pressure

[0027] The plate and frame filtrate was concentrated under reduced pressure at a temperature of 80° C. and a vacuum of -0.095 MPa until the liquid phase content was 34160 ug / ml to obtain a concentrated solution. The concentration yield is 99%.

[0028] (3) Precipitation and separation

[0029] Use sodium hydroxide to adjust the pH value of the concentrated solution to 10.5, stir for 1 h, and stand still at 6°C for 3 h. Vacuum filter the precipitated concentrated solution to obtain a filter cake. While stirring the filter cake, add dilute sulfuric acid to the pH is 6.1, and the acidified solution is ...

Embodiment 2

[0034] (1) Pretreatment of fermentation broth

[0035] Adjust the pH value of the fermented liquid to 2.5 with sulfuric acid, add 3%wt perlite filter aid while stirring, and filter through the plate and frame until no filtrate drips out to obtain the plate and frame filtrate. The plate and frame filtration yield was 95%.

[0036] (2) Concentration under reduced pressure

[0037] The plate and frame filtrate was concentrated under reduced pressure at a temperature of 78 °C and a vacuum of -0.098 MPa until the liquid phase content was 25000 ug / ml to obtain a concentrated solution. The concentration yield is 99%.

[0038] (3) Precipitation and separation

[0039] Adjust the pH value of the concentrated solution to 10.7 with sodium hydroxide, stir for 4 hours, and stand still at 2°C for 3 hours. Vacuum filter the concentrated solution after precipitation to obtain a filter cake. While stirring the filter cake, add dilute sulfuric acid until the pH is 6.4, to obtain the acidifi...

Embodiment 3

[0044] (1) Pretreatment of fermentation broth

[0045] Adjust the pH value of the fermented liquid to 3.5 with sulfuric acid, add 2.5%wt perlite filter aid while stirring, and filter through the plate and frame until no filtrate drips out to obtain the plate and frame filtrate. The plate and frame filtration yield was 92%.

[0046] (2) Concentration under reduced pressure

[0047] The plate and frame filtrate was concentrated under reduced pressure at a temperature of 75 °C and a vacuum of -0.098 MPa until the liquid phase content was 30810 ug / ml to obtain a concentrated solution. The concentration yield is 98.5%.

[0048] (3) Precipitation and separation

[0049] Adjust the pH value of the concentrated solution to 11 with sodium hydroxide, stir for 3 hours, and stand still at 4°C for 3.5 hours. Vacuum filter the concentrated solution after precipitation to obtain a filter cake. While stirring the filter cake, add dilute sulfuric acid to the pH value It was 6.9, and the ac...

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PUM

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Abstract

The invention discloses a preparation method of a colistin sulfate premix. According to the method, colistin is extracted from fermentation liquid by pretreatment of the fermentation liquid, concentration by pressure reduction, separation by precipitation and granulation by a fluid bed, thereby preparing the colistin sulfate and then preparing the colistin sulfate premix. The invention has the advantages of strong specificity, short process, small equipment investment, little waste water, little pollution and high yield of 80-90%.

Description

technical field [0001] The invention relates to the preparation of a polypeptide antibiotic premix, in particular to a preparation method of a colistin sulfate premix. Background technique [0002] Colistin is a multidrug-resistant Gram-negative pathogen (such as Pseudomonas aeruginosa, Pseudomonas aeruginosa ) Polypeptide antibiotics with specific resistance. Colistin contains diaminobutyric acid and has free amino groups, so it is alkaline. It is often used as a salt in clinical practice. Currently, it is commercialized in two forms. Colistin sulfate (colistin sulphate, CS) is one of the forms. Colistin sulfate can be used in animals such as pigs, poultry, cattle, and sheep. Because of its strong antibacterial effect, low residue, and resistance to drugs, it has been approved as a feed additive or veterinary drug by many countries such as China, the United States, the European Union, and Japan. It can treat animal feeding diseases caused by a variety of germs, and can pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/12A61K47/46A61K47/02A23K1/17A61P31/04A23K20/195
Inventor 熊莉孙振华余炜储消和吴雪萍
Owner 浙江拜克生物科技有限公司
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