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Method for preparing CaCO3 nanotube/podophyllum composite material

A composite material and nanotube technology, applied in the field of preparation of new CaCO3 nanotube/podophyllin composite materials, can solve problems such as toxic side effects and limited application, and achieve the effects of uniform distribution, easy product handling and mild reaction system.

Inactive Publication Date: 2013-01-02
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its clinical application is limited due to its serious side effects.

Method used

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  • Method for preparing CaCO3 nanotube/podophyllum composite material
  • Method for preparing CaCO3 nanotube/podophyllum composite material
  • Method for preparing CaCO3 nanotube/podophyllum composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The structure of the self-made gas reaction generator is as Figure 4 As shown, it consists of a constant-speed stirring controller 1, an iron stand 2, a magnetic stirrer 3, an electric stirrer 4, a first glass container 5, an orderly molecular permeable membrane 6 and a constant temperature magnetic stirrer 7, wherein: the first glass The container 5 is placed above the constant temperature magnetic stirrer 7, one end of the second glass container is placed in the first glass container 5, and the bottom of the second glass container is open, and the opening is provided with an ordered molecular permeable membrane 6, and the second glass container There is a certain distance between the bottom and the bottom of the first glass container 5, the second glass container is fixed by the iron stand 2, the stirring rod of the electric mixer 4 is inserted in the second glass container, the electric mixer 4 is connected to the constant speed stirring controller 1, and the second ...

Embodiment 2

[0046] Step 1: Maximum absorption wavelength: Weigh 0.003g of podophyllotoxin, put it in a 10mL volumetric flask, dissolve it with double distilled water and make to volume. The UV-Vis spectrophotometer was used to scan in the range of 200-800 nm, and 285 nm was the maximum absorption wavelength of podophyllotoxin, so the experiment chose to test the concentration of podophyllotoxin in the solution at this wavelength.

[0047] Use the same method for UV detection of podophyllin / nano-CaCO 3 The full ultraviolet spectrum of nanomaterials, observe the absorption of its ultraviolet spectrum, and determine whether there is interference at the sensitive absorption wavelength of podophyllum.

[0048] Drawing of the working curve of podophyllum: prepare 1, 2, 3, 4, 5, 6, 8, 10μg / ml podophyllum standard solutions respectively, and measure the absorbance at 285nm. Fit the working curve.

[0049] Podophyllum / Nano CaCO 3 Determination of drug-loading capacity of composite nanomaterial...

Embodiment 3

[0055] Step 1: Use the normal cell line HEK 293T to detect its cytotoxicity, and select Hela tumor cells for its inhibitory effect on tumor cells.

[0056] Step 2: After pre-cultured for 24 hours, the cells adhered to the wall, and the cell culture medium was used as the negative control group. Three wells in each group were added with different concentrations of PPT solution and CaCO 3 -PPT suspension and CaCO 3 Suspension (concentrations were 5, 10, 20, 40 μg / ml).

[0057] Step 3: After culturing for 24 h, add 20 μl of MTT solution to each well, discard the supernatant after incubation for 4 h, and add 150 μl of DMSO to each well to stop the reaction.

[0058] Step 4: Shake the culture plate horizontally for 30 min, measure the absorbance at 490 nm with an enzyme-linked detector, and calculate the cell survival rate according to the following formula:

[0059] Cell Viability % = A 490(样品) / A 490(对照) ×100%

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Abstract

The invention relates to a preparation method of a CaCO3 nanotube / podophyllum composite material, specifically: first dissolving 4.0 g of o-phenanthroline carrier in 100 ml of chloroform, vigorously stirring, and then cleaning and drying TFOM and putting it into a solution system for soaking After a certain period of time, take it out, clean it with filter paper, put it in the middle of the GRC container, and form a TFOM in the middle, and a solution phase separation system on the left and right. Prepare 0.53%-0.57% sodium carbonate solution and 2.13%-2.17% calcium chloride The solution was placed on the left and right sides of the above-mentioned TFOM respectively, and the crystal growth modifier M was added according to different situations, and the pH value was adjusted to 8. After stirring at a low speed, the solution on one side of the sodium carbonate solution was taken out, and the precipitated product was obtained by centrifugation. Water, acetone, Wash each with absolute ethanol twice, and finally store the product in absolute ethanol. The present invention has stable reaction, simple operation and easy control, low cost and no pollution, easy control of appearance and structure, uniform product distribution, not easy to agglomerate, and high purity. Easy to industrialize.

Description

technical field [0001] The present invention relates to a novel CaCO 3 Preparation method of nanotube / podophyllum composite material. Background technique [0002] One of the goals of materials science that has attracted increasing attention is to construct nanomaterials with symmetrical and complex structures and excellent performance. Micron / nano hollow spheres and tubes are representative substances of this type of cavity materials. They are used in biotechnology, photocatalysis , Photoinduced devices, high-energy electrode materials, gas sensing and other fields have important application values, although there have been many reports on the use of initial crystal unit assembly to form one-dimensional, two-dimensional or three-dimensional hollow structures, such as layered electrostatic attraction, Self-assembly technology, template removal method, etc. However, the conventional techniques for preparing hollow nanomaterials, such as the template method and Ostwald ripen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/48A61K31/365C01F11/18A61P35/00A61P35/02A61P31/18A61P31/20
Inventor 唐静孙冬梅钱文宇汪世龙
Owner TONGJI UNIV
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