Method for constructing insulin resistance animal model

An insulin resistance, animal model technology, applied in biological testing, pharmaceutical formulations, preparations for in vivo testing, etc.

Inactive Publication Date: 2011-11-02
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing animal models of insulin resistance are mainly induced by long-term feeding with high-fat diet, and the experimental period is too long, which is not suitable for screening a large number of insulin resistance prevention and treatment drugs

Method used

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  • Method for constructing insulin resistance animal model
  • Method for constructing insulin resistance animal model
  • Method for constructing insulin resistance animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (2) Glucose oxidase was injected into the tail vein at a dose of 400 U / kg body weight for three consecutive days to obtain an animal model.

[0025] The SD rats were selected, clean, male, weighing 180 ± 220 g, and marked separately; glucose oxidase (400 U / kg) was injected into the tail vein, and the control group was injected with the same amount of normal saline, and they were free to eat and drink. After continuous administration for 3 days, the glucose tolerance and insulin tolerance tests were performed by intraperitoneal injection to obtain an animal model.

[0026] The verification method of the animal model obtained by the above method includes measuring the glucose tolerance level by intraperitoneal injection, the level of insulin tolerance level and the phosphorylation level of protein kinase B stimulated by insulin.

[0027] Blood glucose levels were measured by conventional methods. The test method of intraperitoneal glucose tolerance test (IPGTT) is as f...

Embodiment 2

[0030] Blood glucose meter (Johnson & Johnson), precision electronic balance, 1ml syringe, etc.

[0031] 2. Experimental Animals and Materials

[0032] 2.1 Experimental animals

[0033] SD rats, clean grade, male, weighing 180 ± 220 g, were provided by the Experimental Animal Center of Fourth Military Medical University.

[0034] 2.2 Experimental materials

[0035] Glucose oxidase, normal saline, picric acid, blood glucose test strips, insulin, glucose.

[0036] 3. Experimental method

[0037]Thirty SD rats were randomly divided into normal control group and model group, 15 in each group. Glucose oxidase (dissolved in normal saline, 600U / ml) was injected into the tail vein at a dose of 400U / kg, and the control group was injected with the same volume of normal saline. After continuous administration for three days, on the fourth day, 10 animals were taken from the normal control group and the model group respectively, and were subjected to intraperitoneal injection of gluc...

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Abstract

The invention relates to a method for constructing an insulin resistance animal model. A Sprague Dawley rat is injected with glucose oxidase (400U / kg weight) through tail vein injection to induce to generate insulin resistance. The statistical analysis of ghlcose tolerance and insulin tolerance and the detection of insulin signal transduction prove that the glucose oxidase constructed insulin resistance animal model is feasible, and can serve as a research model for insulin resistance, the pathogenesis of type 2 diabetes and a medicine function mechanism. Compared with the conventional model constructing technology, the method has the advantages of simple modeling method, high modeling efficiency, low cost, short period, high repeatability and the like.

Description

technical field [0001] The invention relates to a method for constructing an insulin resistance animal model, in particular to a method for constructing a rat insulin resistance model. Background technique [0002] The construction of animal models is a very important technique in medical research. Constructing a disease model similar to human disease can overcome the constraints of long incubation period, many influencing factors, slow development and complex pathogenesis of human disease, better reflect the law of disease occurrence and development, and facilitate the study of different diseases. Diseases that are easy to study in humans themselves. Commonly used animal models mainly include spontaneous animal models, transgenic animal models and experimental animal models. Spontaneous animal models and transgenic animal models have high application value, but the high cost of modeling and high requirements for technical breeding conditions limit the general application ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K38/44G01N33/66A01K67/027
Inventor 王欣海春旭
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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