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Endothelial cell selective composite coating material used for cardiovascular stent and preparation method thereof

A composite coating and endothelial cell technology, applied in the interdisciplinary field of disciplines, can solve the problems of reducing the migration and proliferation of endothelial cells, increasing the risk of late thrombosis, and destroying the endothelial layer of blood vessels, so as to achieve efficient and rapid inhibition of growth and endothelialization Insufficient improvement, prevention of restenosis and thrombosis

Inactive Publication Date: 2011-10-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, while the drugs carried by drug-eluting stents treat restenosis caused by excessive proliferation of smooth muscle cells, they will also reduce the migration and proliferation of endothelial cells, damage the endothelial layer of blood vessels, and increase the risk of late thrombosis
Delayed endothelialization and late thrombosis caused by non-selective inhibitory effects of drugs are still key issues restricting its application

Method used

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  • Endothelial cell selective composite coating material used for cardiovascular stent and preparation method thereof
  • Endothelial cell selective composite coating material used for cardiovascular stent and preparation method thereof
  • Endothelial cell selective composite coating material used for cardiovascular stent and preparation method thereof

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preparation example Construction

[0073] The steps of the preparation method of the base layer are as follows:

[0074] 1) Dissolving the biocompatible monomer of the cell membrane biomimetic structure and the polymerizable monomer containing the hydrophobic functional group with a mass ratio of 1:0.1 to 1:10 in 10 to 100 mL of solvent to obtain solution A, wherein the solvent is Toluene, tetrahydrofuran, dimethyl sulfoxide, chloroform, methylene chloride, isopropanol, ethanol, or methanol;

[0075] 2) adding 0.2% to 2% of the free radical initiator azobisisobutyronitrile of the total mass of the biocompatible monomer of the biomimetic structure of the cell membrane and the polymerizable monomer containing the hydrophobic functional group into the solution A, Obtain solution B;

[0076] 3) Deoxygenate solution B with nitrogen or argon for 5 to 30 minutes, heat to 50 to 90 degrees, and react for 6 to 48 hours;

[0077] 4) removing the solvent under reduced pressure, and precipitating with glacial ether or gla...

Embodiment 1

[0090] (1) Prepare the base layer of endothelial cell selective composite coating material

[0091]

[0092] 1) Dissolving 0.5 g of biocompatible monomer MPC with biomimetic structure of cell membrane and 1.5 g of polymerizable monomer SMA with hydrophobic functional group in 30 ml of ethanol to obtain solution A;

[0093] 2) Add 0.04g initiator AIBN to solution A to obtain solution B;

[0094] 3) Deoxygenate solution B with argon for 30 minutes, heat to 60°C, and react for 24 hours;

[0095] 4) removing the solvent under reduced pressure, and precipitating with ice methanol twice to obtain the base layer polymer of the cardiovascular stent;

[0096] 5) dissolving the cardiovascular stent basal layer polymer and rapamycin drug in a tetrahydrofuran solvent with a mass ratio of 1:0.5, configuring a solution with a mass percentage concentration of 0.5%, and ultrasonically treating it to obtain a uniformly dissolved solution C;

[0097] 6) The polyethylene terephthalate (PET)...

Embodiment 2

[0116] (1) Prepare the base layer of endothelial cell selective composite coating material

[0117]

[0118] 1) Dissolving 0.5 g of biocompatible monomer MPC with biomimetic structure of cell membrane and 0.8 ml of polymerizable monomer BMA with hydrophobic functional group in 15 ml of tetrahydrofuran to obtain solution A;

[0119] 2) Add 0.02g initiator AIBN to solution A to obtain solution B;

[0120] 3) Deoxygenate solution B with nitrogen for 30 minutes, heat to 65°C, and react for 30 hours;

[0121] 4) Desolventization under reduced pressure, precipitation with glacial ether for 2 times, to obtain the base layer polymer of cardiovascular stent; NMR results confirm that the obtained product has the expected structure, such as figure 2 as shown in (a);

[0122] 5) dissolving the cardiovascular stent basal layer polymer and paclitaxel drug (Paclitaxel) with a mass ratio of 1:0.8 in a tetrahydrofuran solvent, configuring a solution with a mass percentage concentration o...

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Abstract

The invention discloses an endothelial cell selective composite coating material used for a cardiovascular stent and a preparation method thereof. The coating comprises a medicament-carrying polymer substrate layer and a polymer outer layer for fixing a biological activity molecule, wherein a substrate layer material is obtained by copolymerizing a biocompatible monomer containing a cell membrane biomimetic structure and a polymerizable monomer containing a hydrophobic function group, and then a polymer solution and a medicament solution are co-mixed to construct the substrate layer of the stent; and the polymer outer layer is obtained by copolymerizing the biocompatible monomer containing a cell membrane biomimetic structure, the polymerizable monomer containing the hydrophobic function group and a polymerizable monomer containing a reactive activity function group, then the biological activity molecule for promoting endothelial cell adhesion is introduced through a surface fixing method so that the coating has endothelial cell selectivity. The composite coating material used for a cardiovascular stent coating can maintain non-specificity impedance performance of a stent surface and realize capturing capability of the in vivo endothelial cell in situ; and the obtained coating structure is stable, can adapt an internal environment of a human body, and has favorable application prospect in treating cardiovascular restenosis and cancer.

Description

technical field [0001] The invention relates to an endothelial cell selective composite coating material for cardiovascular stents and a preparation method thereof, and belongs to the interdisciplinary field of materials, biology, physics, chemistry and other disciplines. Background technique [0002] The widespread use of drug-eluting stents provides a good means for efficient and minimally invasive treatment of cardiovascular diseases. However, while the drugs carried by drug-eluting stents treat restenosis caused by excessive proliferation of smooth muscle cells, they also reduce the migration and proliferation of endothelial cells, destroying the endothelial layer of blood vessels, and increasing the risk of late thrombosis. Delayed endothelialization and late thrombosis caused by the non-selective inhibitory effect of drugs are still the key problems restricting its application. In order to solve this critical problem, a series of attempts have been made. [0003] The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/10A61L31/16
Inventor 计剑魏雨纪缨
Owner ZHEJIANG UNIV
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