Method for producing hydrogen by compounding facultative hydrogenogen with anaerobic bacteria

A technology of anaerobic bacteria and fermentation method, which is applied in the field of hydrogen production by compounding facultative hydrogen-producing bacteria and anaerobic bacteria, and can solve the problems of less research on the combination of high-efficiency hydrogen-producing bacteria and less research on single substrates.

Inactive Publication Date: 2011-09-28
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Through patent retrieval, the current research on hydrogen production by mixed bacteria is mainly focused on activated sludge, activated sludge and high-efficiency hydrogen-producing bacteria, such as a method for biological hydrogen production by mixing sludge and organic waste (application number: 200710032658.0 Zhou Shaoqi etc.), there are few studies on the compounding of highly efficient hydrogen-producing bacteria, and there is a patented method for the compounding of strains to degrade cellulose and ferment hydrogen production (application number 200710071696.7 Wang Aijie, etc.), a combination of multi-strain solid-state fermentation straw A method for preparing hydrogen (application number: 01142220.3 Li Xuetuo, etc.), but it is mainly for the treatment of complex waste, and there are few studies on a single substrate

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  • Method for producing hydrogen by compounding facultative hydrogenogen with anaerobic bacteria
  • Method for producing hydrogen by compounding facultative hydrogenogen with anaerobic bacteria
  • Method for producing hydrogen by compounding facultative hydrogenogen with anaerobic bacteria

Examples

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Effect test

Embodiment 1

[0021] The fermentation method of this example is batch fermentation, the substrate glucose is added to the hydrogen production medium, the glucose concentration is 20 g / L, and the initial pH=6.0-7.0. Put an effective volume of 6L hydrogen-producing medium into a 10L fermenter, and sterilize at 115°C for 25min. Inoculate the prepared high-concentration bacterial liquid into the hydrogen-producing medium at a ratio of 1:1, and the inoculum amount of the high-concentration bacterial liquid is 10%, that is, the inoculation volume of the high-concentration bacterial solution is 10% of the medium volume.

[0022] Fermentative hydrogen production process conditions:

[0023] to N 2 Purge the culture medium for 5 minutes to make the fermentation environment close to anaerobic, monitor the pH, temperature and stirring rate of the fermentation liquid phase online, adjust the pH of the fermentation process to maintain 6.0~7.0 by adding dilute NaOH, adjust the stirring rate of the ferm...

Embodiment 2

[0027] Example 2: The difference between this example and Example 1 is that the compounding scheme is facultative hydrogen-producing bacteria Bacteria.E and anaerobic hydrogen-producing bacteria Bacteria.B. The inoculation ratio of high-concentration bacteria solution is 1:1, and the rest are the same Embodiment one.

[0028] Obtain the dynamic hydrogen production curve of embodiment two and refer to the appendix of the description figure 1 , the fermentation hydrogen production cycle is 24h, the cumulative gas production is 29.3L, the fermentation gas phase is composed of two gases, hydrogen and carbon dioxide, of which the hydrogen gas fraction is 54.80%, and the final cumulative hydrogen production is 16.1L; the average hydrogen production rate of the fermentation process is up to 167.25mL / (h L), the decomposition rate of glucose in the liquid phase is 92.00%, and the final hydrogen production per unit substrate is 1.290molH 2 / mol glucose.

Embodiment 3

[0029] Example 3: Two strains of anaerobic hydrogen-producing bacteria Bacteria.P and Bacteria.B were compounded, and the high-concentration bacterial solution was inoculated into the hydrogen-producing medium at a ratio of 1:1, and the rest were the same as in Example 1.

[0030] The dynamic hydrogen production curve obtained in Example 3 is shown in the appendix of the description figure 1 In this example, the fermentative hydrogen production period is 50 hours, and the cumulative gas production is 33.3 L. The fermentation gas phase consists of two gases, hydrogen and carbon dioxide, wherein the hydrogen gas fraction is 60.00%, and the final cumulative hydrogen production is 20.0 L; the fermentation process produces an average The hydrogen rate reaches 86.70mL / (h L), the decomposition rate of glucose in the liquid phase is 93.00%, and the final hydrogen production per unit substrate is 1.604molH 2 / mol glucose. Compared with Example 1 and Example 2, Example 3 has a longer ...

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Abstract

The invention discloses a method for producing hydrogen by compounding facultative hydrogenogen with anaerobic bacteria. One facultative hydrogenogen is compounded with two anaerobic bacteria in a 10L fermentation tank to produce hydrogen according to different compounding schemes. In the batch fermentation hydrogen production process, three strains form four compounding schemes; different compounding schemes are mixed according to the same ratio at the total inoculating concentration of 10 percent to obtain a facultative hydrogenogen and anaerobic bacteria common compounding scheme which has the optimal characteristic of producing hydrogen, the fermentation period of 24h, the hydrogen gas yield of a unit substrate of 1.885molH2/mol glucose and the average hydrogen producing speed of 212.20ml/(h.L); the optimal compounding scheme of the batch fermentation process is applied to a continuous fermentation process; and the glucose is fed in batch at the constant flow speed of 5g/h for 120h and is fermented and cultured for 5 days to further produce hydrogen continuously and stably.

Description

technical field [0001] The invention relates to a method for producing hydrogen by biological fermentation, in particular to a method for compounding hydrogen by using facultative hydrogen-producing bacteria and anaerobic bacteria. Background technique [0002] Biological hydrogen production technology is a technology that uses the metabolic process of hydrogen-producing bacteria to produce hydrogen. It is a clean and low-energy hydrogen production method. At present, domestic and foreign research on hydrogen production by fermentation mainly includes two aspects: one is the research on hydrogen production by pure strains. In order to obtain highly efficient hydrogen-producing fermentation bacteria, domestic and foreign researchers have isolated a large number of hydrogen-producing Most hydrogen-fermenting bacteria are concentrated in the genera Clostridium and Enterobacter. Using pure bacteria with high-efficiency hydrogen production ability, the substrate degradation rate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12R1/01C12P3/00C12R1/145
Inventor 钱春香袁晓明
Owner SOUTHEAST UNIV
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