Method for detecting persistent toxicity of soluble heavy metals by using caenorhabditis elegans

Abstract

The invention discloses a method for detecting persistent toxicity of soluble heavy metals by using caenorhabditis elegans, relating to a method of detecting the toxic effect of the soluble heavy metals under the conditions of long term and low concentration by carrying out the persistent exposure covering multiple successive generations of soluble heavy metals through adopting caenorhabditis elegans. In the invention, the egg initiation concentration which does not affect the normal egg-hatch and is in the presence without heavy metals, and the food initiation concentration which can meet the needs of growth and development of eggs are determined; toxicity transmission strength and transmission paths and the like of the heavy metals under the long-term actual envrionemnt exposure are obtained through mutual comparison of same indexes among different generations under the condition that the exposure time, and the exposure doses, particularly the coexistence of foods and heavy metals and other aspects stay closer to the actual environmental exposure condition. The invention makes up the deficiency of the traditional method for testing toxicity by using caenorhabditis elegans, has aclear pertinence, and supplements and extends the toxicity research method which is under the condition of simulating actual environmental exposure.

Description

technical field [0001] The method for detecting persistent toxicity of soluble heavy metals using Caenorhabditis elegans relates to a method for detecting the toxic effects of heavy metals under long-term and low-concentration conditions by using Caenorhabditis elegans to perform persistent exposure to soluble heavy metals covering multiple consecutive generations. It belongs to the technical fields of environmental protection, ecotoxicology research, and ecological risk assessment. Background technique [0002] Caenorhabditis elegans is very sensitive to changes in the external environment, and environmental stress will change their reproductive speed, life cycle and other developmental characteristics and other behavioral characteristics. Toxicology studies often use C. elegans as a model animal for toxicity testing, but there is no precedent for low-concentration, continuous exposure to multiple generations of persistent toxicity studies. [0003] Methods for measuring g...

AI Technical Summary

Problems solved by technology

Toxicology studies often use C. elegans as a model animal for toxicity testing, but there is no precedent for low-concentration, continuous exposure to multiple generations of persistent toxicity studies

[0003] Methods for measuring generational toxicity of pharmaceutical and personal care substances using C. Some comparative results have been obtained, but there are still differences and deficiencies in this effect and its comparative results relative to the real environment, mainly in the following aspects: First, the exposure time is discontinuous, and the life cycle of each generation of nematodes only accepts exposure for a certain period of time. , is not the exposure of the whole life stage, especially does not cover the developmental stage from eggs to adults of C. elegans, which is more sensitive to changes in the external environment; second, it cannot reflect the real conditions of the coexistence of food and heavy metals in the actual environment, Studies have shown that food can modify the response of C. elegans to heavy metals, and ignoring the effect of food on C. elegans does not provide a strong statement for the risk under real environmental conditions.