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Preparation method of arginine glucoside and application in medicament for treating diabetes

A technology of arginine glycoside and arginine, which is applied in the application of drugs for type 2 diabetes and its complications, the preparation of arginine glycoside, and the treatment of type 1. It can solve the problems of no AFG application and achieve separation and purification The effect of low cost and high synthesis rate

Active Publication Date: 2011-09-14
郑毅男
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After searching, there is no application of AFG in the treatment of type 1 diabetes and type 2 diabetes.

Method used

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  • Preparation method of arginine glucoside and application in medicament for treating diabetes
  • Preparation method of arginine glucoside and application in medicament for treating diabetes
  • Preparation method of arginine glucoside and application in medicament for treating diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] 1. Put 1g of arginine and 2g of maltose into 35ml of glacial acetic acid, add 0.1g of sodium sulfite, and react for 30 minutes at 50°C to obtain the total reaction product, which is concentrated at low temperature and vacuum to an extract, and dried on silica gel Mix the sample with the method, pass through a silica gel (200-300 mesh) wet column, elute with 50-90% ethanol, collect each fraction with an automatic fraction collector, check with thin-layer chromatography, collect the eluent fraction with an Rf value of 0.2, and use a low-pressure Concentrate to extract, freeze-dry into powder, then dissolve the powder in 0.2% acetic acid aqueous solution of 5 times the amount, pass through polyacrylamide (Bio-gel P-II) column, elute with 0.2% acetic acid aqueous solution, and use automatic part The fractions were collected by the collector, checked by thin-layer chromatography, and the eluate with an Rf value of 0.2 was collected, concentrated under reduced pressure, and fr...

Embodiment 2

[0110] Put 1 g of arginine and 2 g of maltose into 40 ml of glacial acetic acid, add 0.1 g of sodium bisulfite, and react at 70° C. for 30 minutes to obtain the total reaction product, and then treat it in the same manner as in Example 1 to obtain 0.3 g of AFG .

Embodiment 3

[0112] Synthesis of AFG

[0113] Take 1g of arginine and 2g of maltose, put them into 50ml of glacial acetic acid, add 0.2g of sodium hydrosulfite, and react at 85°C for 30 minutes to obtain the total reaction product, and then treat it in the same way as in Example 1 to obtain 0.5g of AFG.

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Abstract

The invention discloses a preparation method of arginine glucoside, which comprises the following steps: adding arginine and maltose into glacial acetic acid, adding a maillard reaction inhibitor, reacting for 10 min to 2 hours to obtain a total reactant; concentrating the total reactant under a low-temperature and vacuum condition to obtain an extract, stirring the extract with silica gel by a dry process, evaporating acetic acid, applying the extract to a 200-300-mesh silica gel column, eluting the column with 50-90% ethanol, collecting each flowing component, detecting the components by thin-layer chromatography, collecting a portion of the eluate with a Rf value of 0.2, concentrating the eluate under a low pressure, freeze-drying the eluate into powder, dissolving the powder in an aqueous acetic acid solution, applying the solution to a polyacrylamide column, eluting the column with the aqueous acetic acid solution, collecting each flowing component, detecting the components by thin-layer chromatography, collecting an eluate with a Rf value of 0.2, concentrating the eluate under a reduced pressure, and freeze-drying to obtain a product. The preparation method of the invention can increase the AFG conversion rate in the synthesis, reduce the generation of reaction by-products, decrease the generation of melanoid which is an end product of the maillard reaction, and thus greatly reduce the cost of the separation and the purification of AFG; the invention provides an medical effect of AFG on the prevention and the treatment of type 1 diabetes and type 2 diabetes.

Description

technical field [0001] The invention discloses a preparation method of arginine glycoside, and also discloses the application of AFG in treating type 1 and type 2 diabetes mellitus and its complications, and belongs to the technical field of traditional Chinese medicine. Background technique [0002] Diabetes is a chronic disease that requires long-term treatment. Western medicine treats diabetes by oral hypoglycemic drugs such as glyburide, metformin, rosiglitazone and other drugs, and insulin is given when necessary, mainly by reducing blood sugar or regulating metabolic secretion disorders to achieve the purpose of treating the disease. However, long-term use of hypoglycemic drugs can lead to severe drug resistance, anaphylactic shock, hypoglycemia, and adverse reactions such as liver and kidney damage. For this chronic wasting disease, not only rely on drug treatment, but also require changing bad living habits and taking supplementary therapeutic agents. [0003] The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7016A61P3/10C07H5/06C07H1/00
Inventor 郑毅男杨世杰
Owner 郑毅男
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