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Method for preparing chitosan oligosaccharide monomers by gel chromatography

A gel chromatography and chitosan oligosaccharide technology, which is applied in the field of separation and purification of a single component of chitosan oligosaccharides, can solve the problems of complex preparation process and high price of chitosan oligosaccharide monomers, and achieve high purity, easy preservation, and Handle simple effects

Active Publication Date: 2011-09-07
惠州长龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the complicated preparation process, at present, the price of chitosan oligosaccharide monomer is extremely expensive, and there is an urgent need for a simple and easy process to prepare chitosan oligosaccharide monomer pure product, so as to promote the basic research and applied research of the chitosan oligosaccharide industry. Play a pivotal role in promoting the chitin industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1. A kind of method that gel chromatography prepares chitosan oligosaccharide monomer, its step is as follows:

[0017] (1) Preparation of chitosan oligosaccharide solution: using chitosan oligosaccharides with a degree of polymerization of 2-15 and of which the degree of polymerization is 2-7 accounts for more than 90% of its mass and the degree of deacetylation is greater than 90% as raw materials, the chitosan oligosaccharides Sugar is dissolved in double distilled water at a mass concentration of 20%, and after it is completely dissolved, it is suction filtered, and the filtrate is the chitosan oligosaccharide solution;

[0018] (2) Load the chitosan oligosaccharide solution on the gel chromatography column and elute with double distilled water at a flow rate of 15ml / h; use an automatic partial collector to collect one tube every 30 minutes, take 0.1ml from each tube, and precipitate with acetone , dissolve the precipitate with distilled water, and then use...

Embodiment 2

[0020] Example 2. A kind of method that gel chromatography prepares chitosan oligosaccharide monomer, its step is as follows:

[0021] (1) Preparation of chitosan oligosaccharide solution: using chitosan oligosaccharides with a degree of polymerization of 2-15 and of which the degree of polymerization is 2-7 accounts for more than 90% of its mass and the degree of deacetylation is greater than 90% as raw materials, the chitosan oligosaccharides Sugar is dissolved in double distilled water at a mass concentration of 70%, and after being completely dissolved, it is suction filtered, and the filtrate is chitosan oligosaccharide solution;

[0022] (2) Load the chitosan oligosaccharide solution on the gel chromatography column and elute with double distilled water at a flow rate of 100ml / h; use an automatic partial collector to collect one tube every 30 minutes, take 0.1ml from each tube, and acetone precipitate , dissolve the precipitate with distilled water, and then use TLC to ...

Embodiment 3

[0024] Example 3. A kind of method that gel chromatography prepares chitosan oligosaccharide monomer, its step is as follows:

[0025] (1) Preparation of chitosan oligosaccharide solution: using chitosan oligosaccharides with a degree of polymerization of 2-15 and of which the degree of polymerization is 2-7 accounts for more than 90% of its mass and the degree of deacetylation is greater than 90% as raw materials, the chitosan oligosaccharides Sugar is dissolved in double distilled water at a mass concentration of 50%, and after it is completely dissolved, it is suction filtered, and the filtrate is the chitosan oligosaccharide solution;

[0026] (2) Load the chitosan oligosaccharide solution on the gel chromatography column and elute with double distilled water at a flow rate of 60ml / h; use an automatic partial collector to collect one tube every 30 minutes, take 0.1ml from each tube, and acetone precipitate , dissolve the precipitate with distilled water, and then use TLC ...

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Abstract

The invention relates to a method for preparing chitosan oligosaccharide monomers by gel chromatography, which is characterized by comprising the following steps of: dissolving 20 to 70 mass percent chitosan oligosaccharide in double distilled water, and performing suction-filtering to obtain filtrate, namely chitosan oligosaccharide solution after the chitosan oligosaccharide is completely dissolved; loading the chitosan oligosaccharide solution to a gel chromatographic column, eluting by using the double distilled water, collecting 0.1ml in one pipe every 30 minutes by using an automatic partial collector, dissolving sediment in distilled water after acetone is settled, and quickly detecting by thin layer chromatography (TLC) to determine the types of the chitosan oligosaccharide monomers contained in each pipe; taking a small amount of liquid from each pipe, freeze-drying, weighing solid matters to determine the concentration of chitosan oligosaccharide contained in each pipe, and drawing an elution curve; separating to obtain solution containing the chitosan oligosaccharide monomers; and concentrating under reduced pressure, and freeze-drying to obtain various chitosan oligosaccharide monomers. The method is low in cost, easy and convenient to operate and mature in technical route, and the prepared chitosan oligosaccharide monomers have high purity and can be used as standard products and research biochemical reagents.

Description

technical field [0001] The invention relates to a method for separating and purifying a single component of chitosan oligosaccharide, in particular to a method for preparing chitosan oligosaccharide monomer by gel chromatography. Background technique [0002] Chitooligosaccharide is an oligosaccharide composed of 2-15 glucosamine units, and its extensive functions in the fields of medicine, agriculture, and light industry have been generally recognized by the academic circles and the market; chitooligosaccharide as a whole (2-15 Sugar mixture), people basically know its functions, but with the development of technology, scientists should gradually understand which part works and what kind of function it plays, so that different products can be developed for different functions , and can guarantee the controllability and stability of the product. In order for the scientific research in this industry to continue to deepen and for the healthy development of this industry, ther...

Claims

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Application Information

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IPC IPC(8): C07H5/06C07H1/06C08B37/08
Inventor 陈列欢肖辉李燕曾毅伟
Owner 惠州长龙生物技术有限公司
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