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Method for extracting quercetin-7-0-rhamnoside

A rhamnoside and extraction method technology, which is applied in the field of extracting quercetin-7-O-rhamnoside monomers, can solve the problems of not providing an extraction method, and achieve high extraction rate, high purity, and extraction The effect of simple process

Inactive Publication Date: 2005-01-19
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Previous studies failed to provide a feasible extraction method for industrial production of quercetin-7-O-rhamnoside monomer that can be obtained in large quantities

Method used

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  • Method for extracting quercetin-7-0-rhamnoside
  • Method for extracting quercetin-7-0-rhamnoside
  • Method for extracting quercetin-7-0-rhamnoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Cut the medicinal material of Dianthus chinensis into decoction pieces, add 8 times the amount of water, decoct and extract three times, one hour each time, and filter; the filtrate is concentrated into an extract, passed through a polyamide column, eluted with 40% ethanol, and then washed with 70% ethanol Elution: collect 70% ethanol eluent, ethanol is recovered from the eluent to obtain a precipitate, the precipitate is mixed with silica gel, put on a silica gel column, and eluted with ethyl acetate, and ethyl acetate is recovered from the eluent to obtain quercetin -7-O-rhamnoside monomer. The yield was 82.3%.

Embodiment 2

[0027] Grind the medicinal material of Dianthus chinensis, add 100°C water to submerge the medicinal material, extract three times, each time for 1 hour, filter, and combine the filtrate; concentrate the filtrate, add absolute ethanol until the ethanol concentration is 70%, leave it overnight, filter, and supernatant Ethanol is recovered. Pass the extract through a polyamide column, elute with 40% ethanol first, then elute with 70% ethanol; collect the eluate from 70% ethanol, recover ethanol from the eluate, mix the precipitate with silica gel, put it on a silica gel column, and Ethyl acetate was eluted, and ethyl acetate was recovered from the eluent to obtain quercetin-7-O-rhamnoside monomer. The yield was 84.5%.

Embodiment 3

[0029] Cut the medicinal material of Dianthus chinensis into decoction pieces, extract twice with 70°C water for 3 hours each time, filter, and combine the filtrate; the filtrate is concentrated into an extract, add 95% ethanol to the extract until the ethanol concentration is 60%, and let it stand overnight , produce precipitation, filter, remove precipitation, ethanol is recovered from the filtrate, the extract is passed through a polyamide column, first eluted with 40% ethanol, and then eluted with 70% ethanol; the 70% ethanol eluate is collected, and ethanol is recovered from the eluate , After mixing the precipitate with alumina, put it on the alumina column, elute with ethanol, recover ethanol from the eluent, and obtain quercetin-7-O-rhamnoside monomer. The yield was 78.5%.

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Abstract

The present invention relates to a method for extracting quercetin-7-O-rhamnoside monmer from Chinese medicinal material containing quercetin-7-O-rhamnoside, which includes the following steps: pulverizing Chinese medicinal material, extracting with water for once to several times, filtering, combining filtrates, concentrating to obtain extract, alcohol precipitation, column separation at twice, eluting with organic solvent, volatilizing solvent from eluant. Its preparation process is simple, and said monomer content in its product is up to above 95%.

Description

technical field [0001] The invention relates to a method for extracting quercetin-7-O-rhamnoside monomer from the Chinese medicinal material C. chinensis. Background technique [0002] Quercetin-7-O-rhamnoside is a kind of flavonoids, which exists in the Chinese herbal medicine Di ear. Hypericum japonicum Thunb. is used to treat hepatitis, and its quercetin-7-O-rhamnoside is one of its main active ingredients. [0003] The way to obtain quercetin-7-O-rhamnoside can be obtained by extracting from the medicinal material Dirnaria chinensis. However, because Chinese herbal medicines contain various flavonoids, polysaccharides, tannins, coumarins and other substances, and flavonoids also contain many substances with similar structures. To obtain quercetin-7-O-rhamnoside monomers, it is necessary to separate quercetin-7-O-rhamnoside from other substances. Previous studies have failed to provide a feasible extraction method for industrial production that can obtain quercetin-7-O...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/08C07H17/07
Inventor 苏薇薇王永刚彭维吴忠唐西杨立伟方铁铮宋晓虹
Owner SUN YAT SEN UNIV
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