Multi-wavelength micro illumination device

Abstract

The invention provides a multi-wavelength micro illumination device. In the device, a laser pulse output by a picosecond pulse laser or a femtosecond pulse laser is incident to a nonlinear photonic crystal fiber to obtain a broadband laser source; the pulsed laser beam output by the broadband laser source is incident to an acoustooptical modulator to obtain a laser pulse beam with a repetition frequency; a beam splitter and a plurality of reflectors are arranged on the optical path of the laser pulse beam; the laser pulse beam is divided into n parallel output beams (n is a natural number equal to or larger than 2); a dispersion prism or a diffraction grating, a lens (a), a spatial light modulator and a lens (b) are sequentially arranged on the optical path of each output beam; and (n-1) output beams respectively pass through a right angle prism after passing through the dispersion prism or the diffraction grating, the lens (a), the spatial light modulator and the lens (b) to obtain n parallel output beams with different delay time. The multi-wavelength micro illumination device provided by the invention can provide laser wavelengths with a wide waveband range from near ultraviolet to mid-infrared and high spectral energy density above 1 nJ / nm, and is suitable for excitation of the most of fluorescence and Raman samples. Additionally, the plurality of laser wavelengths are from the same laser source so as to obviate the problem of synchronous time maladjustment, and the apparatus cost can be reduced greatly by using one pulse laser.

Description

technical field [0001] The invention relates to a multi-wavelength microscopic illumination device, which belongs to the field of optical microscopic imaging. Background technique [0002] With its non-invasive three-dimensional imaging capability, optical microscopic imaging still accounts for about 80% of life science imaging research (Stefan W. Hell, et al., Science, 316, 25 (2007)). With the development of optical microscopy imaging technology, multicolor excitation fluorescence microscopy (Amit Agrawal, et al., PNAS, 105, 9, 3298-3303 (2008)), stimulated radiation depletion super-resolution microscopy (Thomas A .Klar, et.al., Physical review E., 64, 066613(2001); Marcus Dyba, et.al., Phys.Rev.Lett., 88(16), 163901(2002)), and coherent inverse Stokes Raman scattering microscopy (Andreas Zumbusch, et. al., Phys. Rev. Lett., 82, 20, 4142-4145 (1999)), etc. All these microscopic imaging techniques require multi-wavelength excitation. The traditional multi-wavelength excit...

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Problems solved by technology

[0003] The purpose of the present invention is to provide a multi-wavelength micro-illumination device; in the device, multiple excitation wavelengths are simultaneously provided by the same pulse laser, which overcomes the shortcoming of low energy density of non-laser light sources and overcomes the need for synchronization of multiple pulse lasers. The shortcomings of misalignment and limited number of wavelengths overcome the shortcomings of low energy density of continuous output lasers and sample denaturation or even damage caused by continuous excitation of samples

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