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New alicyclobacillus strain and application thereof in bioleaching

A technology of bacillus and alicyclic acid, which is applied to the new strain of alicyclic bacillus and its application in bioleaching, can solve the problems of slow growth, low quantity, large output, etc., and achieve high leaching rate, The effect of short leaching time and broad economic prospects

Inactive Publication Date: 2012-06-13
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the microbial leaching strains used in biometallurgy are all chemoautotrophic microorganisms, whose growth is slow and low in number, and their growth and reproduction will be inhibited by the organic matter in the culture system. Therefore, it is impossible to increase the growth rate and number of strains by adding organic matter. Generally, it is mostly used for leaching precious metals such as gold and uranium, or as heavy metal leaching, tailings, and waste ore treatment.
However, it cannot be popularized and applied to the leaching of many low-value, high-yield and widely used metals such as iron and manganese.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Utilize Alicyclobacillus to leach pyrite (small-scale experiment)

[0031] 1) Prepare seed solution:

[0032] Select BAM medium, adjust the pH to 1.5, and expand the culture of bacteria at 25°C and 150rpm to OD 600 When the value of is 0.5-1.0, the culture is stopped, and the seed solution is obtained.

[0033] BAM medium contains the following components: ammonium sulfate 0.1-0.5g / L, potassium dihydrogen phosphate 1-3g / L, magnesium sulfate 0.1-0.5g / L, calcium chloride 0.1-0.5g / L, ferrous sulfate 40-200g / L

[0034] 2) Domestication of bacterial strains in seed solution

[0035] Take 50ml of seed solution and centrifuge at 5000 rpm for 5min, discard the supernatant, resuspend the pellet with 5ml of BAM liquid medium, and then add to 95ml of BAM medium containing 1% pyrite by mass ratio without ferrous sulfate In the condition of 25℃, 150rpm for acclimatization, when the culture medium OD 600 After reaching 0.5-1.0, transfer to the BAM medium of the nex...

Embodiment 2

[0038] Embodiment 2: Utilize Alicyclobacillus to leach pyrite (small-scale experiment)

[0039] Compared with Example 1, the temperature of cultivating and acclimating Alicyclobacillus BM strain is increased to 35°C, the rotating speed is changed to 200rpm, the pH value of the medium is adjusted to 5.0, and the conditions during ore leaching are the same as those for cultivation , after leaching for 24 hours, the total number of iron ions in the leaching solution reached 2.558g, and the leaching efficiency was 85.2%.

Embodiment 3

[0040] Embodiment 3: Utilize Alicyclobacillus to leach pyrite (small-scale experiment)

[0041] Compared with Example 2, the optimum growth temperature of Alicyclobacillus BM strain is set at the temperature of cultivation and domestication, and the pH of the culture medium is adjusted to 3.0. After 24 hours of leaching, the leaching liquid The total iron ion number reached 2.630g, and the leaching efficiency was 87.6%. This is because the acidic conditions and culture temperature of the culture medium are the most suitable for the growth of the Alicyclobacillus BM strain, so that the number of bacteria in the bacterial liquid is the largest, and the ability of ore leaching is also the largest.

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Abstract

The invention relates to a new alicyclobacillus strain and an application thereof in bioleaching. The conservation number of the screened alicyclobacillus strain is CGMCC 4500; and the strain is cultured at the temperature of 25-35 DEG C and grows within the pH range of 1.5-5.0. The strain can be applied in the bioleaching field such as pyrite leaching. The alicyclobacillus strain has an acidophilic character, and can grow by taking ferrous ions as an energy matrix and fixed CO2 as a carbon source and meanwhile can rapidly grow by virtue of organicnutrition, thus the strain is suitable for large-scale amplification culture so as to be applied in the bioleaching field. Studies show that the new alicyclobacillus strain has the advantages of high leaching rate, short leaching time, no environmental pollution and the like, and metal ions therein have high tolerance and are free from inhibition of organic matters in the system.

Description

technical field [0001] The invention belongs to the technical field of microbial leaching, and in particular relates to a new strain of Alicyclobacillus and its application in bioleaching. Background technique [0002] With the continuous development and utilization of mineral resources, rich ore and high-quality ore resources are becoming increasingly scarce, while the proportion of low-content ore increases accordingly, causing conventional treatment methods to face severe challenges in both technical and economic aspects. Biometallurgy has the characteristics of high leaching efficiency, low energy consumption, simple process and environmental protection, especially for some low-grade rare mineral deposits, which can achieve leaching efficiency that cannot be achieved by traditional physical and chemical processes. focus of attention. [0003] At present, the microbial leaching strains used in biometallurgy are all chemoautotrophic microorganisms, which grow slowly and h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C22B3/18C12R1/01
CPCY02P10/20
Inventor 韩一凡郑承纲张小霞温丹黄志勇
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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