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Method for detecting water inherent toxicity by using single cell gel electrophoresis test

A single-cell gel and genetic toxicity technology, which is applied in the field of water toxicity detection, can solve the problems of difficult unification and insufficient intuitive toxicity, and achieve the effects of easy unification of test results, quantification, and accurate water toxicity

Inactive Publication Date: 2011-07-20
济南市供排水监测中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the SCGE test method has long been used as a normative method compared with the Ames test method and the micronucleus test method, the test method and result analysis of the single-cell gel electrophoresis test still need to be further standardized. When analyzing the results, only qualitative methods are used to judge toxic substances. The magnitude of genotoxicity, different people have different judgment standards, it is difficult to unify, and only the blank sample is used as the control in the experiment, the toxicity reaction is not intuitive enough, so looking for an intuitive, clear, and easy-to-unify method to detect water quality toxicity It is the demand for the development of quantitative evaluation of toxic substances in water quality

Method used

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  • Method for detecting water inherent toxicity by using single cell gel electrophoresis test

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Take daily urban drinking tap water as the water sample to be tested, and carry out concentration and enrichment treatment on the water sample. The process is as follows: take 100L water sample, and use the activated XAD-2 resin for adsorption at a rate of 40mL / min. After adsorption, use acetic acid Ethyl ester was eluted, and the eluate was concentrated to 10 mL at 50°C.

[0038] This specific embodiment adopts the following steps:

[0039] (1) Select the lymphocytes of the spleen of mice as the indicator organism, and take 6 concentration points within the range of chromium ion concentration 0.025-0.5mg / L to treat the mice for 5 days respectively. The ion concentrations are 0.025mg / L, 0.05mg / L, 0.1mg / L, 0.15mg / L, 0.25mg / L, 0.5mg / L;

[0040] (2) Preparation of single cell suspension: Cut out the spleen of the mouse, rinse with phosphate buffer, add 1mL of phosphate buffer, cut it into pieces with ophthalmic scissors, absorb the supernatant, add lymphocyte extract to t...

Embodiment 2

[0050] Take the influent water from the daily urban water plant as the water sample to be tested, and carry out concentration and enrichment treatment on the water sample. The process is as follows: take 40L water sample, and use the activated XAD-2 resin for adsorption at a rate of 40mL / min. After that, it was eluted with ethyl acetate, and the eluate was concentrated to 10 mL at 50°C.

[0051] Adopt and embodiment 1 method to draw Cr 6+ Standard curve for olive tail distance, the curve with figure 1 Basically the same, and then the concentrated and enriched water samples were exposed to the mice, the steps were the same as in Example 1, the olive tail distance of the obtained water samples to be tested was 50.1, and the experimental results were substituted into Cr 6+ To the standard curve of olive tail distance, the water quality toxicity of this water sample is equivalent to 0.225mg / LCr 6+ toxicity.

Embodiment 3

[0053] Take the effluent of the productive test process of the daily urban water plant as the water sample to be tested, and carry out concentration and enrichment treatment on the water sample. The process is as follows: take 20L water sample, and use the activated XAD-2 resin for adsorption at a rate of 40mL / min , after adsorption, it was eluted with ethyl acetate, and the eluate was concentrated to 10 mL at 50 °C.

[0054] Adopt and embodiment 1 method to draw Cr 6+ Standard curve for olive tail distance, the curve with figure 1 Basically the same, and then the concentrated and enriched water sample was poisoned to the mice, the steps were the same as in Example 1, the olive tail distance of the obtained water sample to be tested was 40.1, and the experimental results were substituted into Cr 6+ To the standard curve of olive tail distance, the water quality toxicity of this water sample is equivalent to 0.102mg / LCr 6+ toxicity.

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Abstract

The invention provides a method for detecting water inherent toxicity by using single cell gel electrophoresis test, which comprises the following steps: performing contaminating treatment on a mouse by using Cr6+ solutions with different concentrations; respectively performing single cell gel electrophoresis test on the splenic lymphocyte of the contaminated mouse so as to obtain olive tail moments under the condition of contamination with the Cr6+ solutions with different concentrations; drawing a standard curve of Cr6+ to olive tail moments; performing contaminating treatment on the mouse with a concentrated and enriched water sample to be detected; measuring an olive tail moment under the condition of the water sample treatment by using the same method; and substituting the olive tail moment in the standard curve to obtain the water toxicity of the water sample to be detected, which is represented by the Cr6+ concentration. By using the method, the Cr6+ is chosen as the standard toxic matter and the quantification evaluation on the water toxicity is realized by using the curve of Cr6+ to the olive tail moment. By using the method provided by the invention, the water inherent toxicity can be measured more directly and accurately, the result is easily unified, the repeatability is good, and the state of water pollution can be judged.

Description

technical field [0001] The invention relates to a method for detecting water quality toxicity, in particular to a method for quantitatively detecting water quality genotoxicity by using a single-cell gel electrophoresis test. Background technique [0002] In order to ensure drinking water safety and reduce the threat of environmental degradation to drinking water quality, people often use various methods to evaluate water quality to effectively grasp the health risks caused by drinking water. [0003] Commonly used methods for detecting water toxicity include Ames test, micronucleus test and single cell gel electrophoresis (SCGE) test. The SCGE test method is a new technology developed in recent years for detecting DNA strand breaks in a single mammalian cell. It has the advantages of sensitivity, simplicity, and less required samples, and has been widely used. Compared with the Ames test and micronucleus test has certain advantages. Although the SCGE test method has long ...

Claims

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Application Information

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IPC IPC(8): G01N27/447G01N21/64
Inventor 贾瑞宝宋艳张诺孙韶华
Owner 济南市供排水监测中心
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