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Method for extracting desertliving cistanche extract and application thereof

A technology of Cistanche deserticola extract and extraction method, which is applied in the field of medicinal plant application research, can solve problems not related to the anti-intestinal cancer effect and mechanism of Cistanche deserticola, and achieve the effects of reducing intestinal hyperplasia, increasing weight, and reducing infection

Inactive Publication Date: 2011-07-06
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many articles about the pharmacology and pharmacological effects of Cistanche, but none of them involve the anti-cancer effect and mechanism of Cistanche

Method used

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  • Method for extracting desertliving cistanche extract and application thereof
  • Method for extracting desertliving cistanche extract and application thereof
  • Method for extracting desertliving cistanche extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the preparation of cistanche extract

[0021] Grind the dried fleshy stem of Cistanche deserticola into powder, weigh 300g of the powder, add 500g of water, extract by reflux for 2 hours, filter, add 500g of water to the dregs, extract by reflux twice, extract for 1 hour each time, combine the extracts three times, The extract was dried by a lyophilizer, and a brown powder was obtained after drying, which was the extract of Cistanche deserticola, which was prepared into a 300ug / ml aqueous solution with distilled water for subsequent use.

Embodiment 2

[0022] Embodiment 2: the pharmacological experiment of Herba Cistanche

[0023] Intestinal cancer model mice were fed with 300ug / ml aqueous solution of Cistanche deserticola extract for 3 months. After the experiment was over, the changes of the spleen were observed. Small intestine and large intestine tissues were taken, fixed in 10% formalin, embedded in paraffin, stained with H&E, and the type and number of cancerous changes were observed under a microscope.

[0024] According to the tissue, shape and degree of invasion of cancer, it is divided into 3 categories: hyperplasia, adenoma, and carcinoma. Hyperplasia is typically characterized by thickened mucosa and elongated intestinal glands, but with normal upper epithelial cells and normal goblet cell architecture. Adenomas are characterized by thickened muscularis, decreased goblet cells, and differentiated intestinal glands in the submucosa. Colorectal cancer is characterized by marked disruption of the muscularis tissue...

Embodiment 3

[0025] Example 3: Cistanche Herba Extract: Activation of the immune system experiment

[0026] Intestinal cancer model mice were fed with 300ug / ml aqueous solution of Cistanche deserticola extract for 3 months. The changes of the immune organs of the mice in the drug-feeding group and the control group were compared and splenocytes were isolated; NK cells and macrophages in splenocytes were labeled with CD49 and F4 / 80 antibodies, respectively.

[0027] The results showed that the spleen weight of the mice in the drug-feeding group was higher than that of the control group, and the number of splenocytes was also higher than that of the control group, and there was a significant difference (see Table 1); the proportion of NK cells in the mice in the drug-feeding group was significantly higher than that of the control group. The proportion of macrophages was higher than that of the control group, but there was no significant difference (see Table 2).

[0028] Table 1 Effect of C...

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PUM

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Abstract

The invention discloses a method for extracting a desertliving cistanche extract and application thereof, which belong to the technical field of application research of medicinal plants. The extract derives from Cistanche deserticola or Cistanche tubulosa of Cistanche, Orobanchaceae. The method comprises the following steps of: grinding dry fleshy stems of desertliving cistanche into powder, performing reflux extraction, filtering, performing reflux extraction on decoction dregs again, freezing and drying in a dryer to obtain brown powder, namely the desertliving cistanche extract. The desertliving cistanche extract can activate the immune system, increase the weight of immune organs, namely spleens, treat intestinal cancer, reduce intestinal hyperplasia, protect normal intestinal epidermal cells, and reduce the infection of Helicobacter pylori in intestines.

Description

technical field [0001] The invention belongs to the technical field of medicinal plant application research, and in particular relates to an extract of Cistanche deserticola, an extraction method and its application in the preparation of medicines for treating intestinal cancer. Background technique [0002] Cistanche has the effects of nourishing the liver and kidney, benefiting essence and blood, moistening the intestines and laxative, and returns to the kidney and large intestine meridian. From the point of view of meridian distribution, Cistanche has a multiplier effect on the treatment of colorectal lesions, but modern pharmacological and pharmacological studies mainly focus on the laxative effect of Cistanche, while there are few studies on the effects of other colorectal lesions, such as Cistanche water. The extract can significantly increase the intestinal propulsion of mice, shorten the defecation time of mice, and inhibit the absorption of water by the large intest...

Claims

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Application Information

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IPC IPC(8): A61K36/64A61P1/00A61P35/00
Inventor 郭玉海贾亚敏朱艳霞
Owner CHINA AGRI UNIV
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