HSV-I and HSV-II IgM antibody joint inspection kit and preparation method thereof
A herpes simplex virus and kit technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome operation process, inconvenient and fast detection, etc., and achieve the effect of simplified operation process, rapid response and simple operation
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[0034] Example 1: Preparation of Herpes Simplex Virus Type I, Type II IgM Antibody Joint Detection Kit
[0035] 1. Preparation of mouse anti-human IgM monoclonal antibody
[0036] Using monoclonal antibody preparation method, hybridoma cell lines were routinely cultured and passaged in vitro, injected with human IgM into the peritoneal cavity of BALB / C mice, collected ascites, and purified mouse anti-human IgM monoclonal antibody by affinity chromatography.
[0037] 2. Preparation of Herpes Simplex Virus Type I Antigen and Herpes Simplex Virus Type II Antigen
[0038] Culture human embryonic diploid cells to a single layer, inoculate the standard strain of herpes simplex virus type I, wait for 2-3 days after the inoculation of the type I strain of herpes simplex virus, 100% of the cells are pathological, collect the culture medium and / pathological cells, PBS Wash 3 times and add glycine-sodium hydroxide buffer, pH 9.0. After freezing and thawing for 3 times, sonicate, take t...
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