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Chemiluminescence quantitative detection kit for carbohydrate antigen 242

A sugar chain antigen, chemiluminescence technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, measurement devices, etc., can solve the problems of high price, difficult to popularize, complicated operation, etc., and achieve stable detection ability , the effect of easy promotion

Inactive Publication Date: 2011-06-15
上海裕隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, foreign automatic chemiluminescent enzyme immunoassay analyzers and matching kits are mostly used in closed systems, which are expensive and complicated to operate, and are not easy to promote in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Preparation of CA 242 Chemiluminescent Quantitative Detection Kit

[0019] (1) Preparation of anti-CA 242 antibody-coated plates

[0020] a. Coating: Take 1mol / L Na 2 HPO 4 77.4ml and 1mol / L NaH 2 PO 4 22.6ml and mix evenly, add deionized water to 1000ml to form a 10-fold coating solution, dilute ten times before use, add appropriate amount of CA 242 monoclonal antibody (purchased from Fujirebio) and mix well, then add to the wells of the microplate medium, 100μl / well, 4°C for 16 hours;

[0021] b. Sealing: Discard the coating solution, pat dry on absorbent paper, add 3% BSA and 0.05% preservative (Proclin TM 300) of phosphate buffer (pH 7.4), 200 μl / well, 37 ° C for 2 hours;

[0022] c. Seal the bag: Discard the sealing solution, pat it dry on absorbent paper, pump it in a vacuum oven for 5 hours at room temperature, immediately vacuum seal the bag, check for air leakage, and re-seal the bag if there is a label. Store at 2-8°C.

[0023] (2) Prepara...

Embodiment 2

[0038] Example 2: Method of use of CA 242 chemiluminescence quantitative detection kit

[0039] 1. Reagent and Sample Preparation

[0040] (1) Reagent preparation

[0041] a. Equilibrate the kit at room temperature (18-26°C) for 20 minutes.

[0042] b. Take out the concentrated washing solution from the kit, dilute it 1:20 with fresh purified water and add it to the washing solution bottle of the plate washer.

[0043] (2) Sample preparation

[0044] The qualified serum or plasma to be tested should be equilibrated at room temperature (18-26° C.) for 20 minutes before use.

[0045] 2. Operation steps

[0046] a. Take out the required CA 242 antibody-coated microwell plate and place it on the microwell rack

[0047] b. Add calibrators 1-6, quality controls 1, 2 and samples to be tested, 25 μl per well.

[0048] c. Add enzyme marker 100 μl / well.

[0049] d. Gently oscillate to mix.

[0050] e. Incubate at room temperature for 120 minutes.

[0051] f. Discard the waste l...

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PUM

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Abstract

The invention discloses a chemiluminescence quantitative detection kit for a carbohydrate antigen 242 (CA242). The kit comprises a CA242 detection reaction plate, an enzyme conjugate, a luminescent substrate, a calibrator, a quality control material and washing concentrate, wherein the reaction plate is coated with a CA242 antibody. The kit can specifically and quantitatively detect the content of the CA242 in patient serum, and is used for the auxiliary diagnosis and prognosis of malignant tumors of a digestive tract such as pancreatic cancer and the like, and monitoring of curative effects. Compared with the conventional enzyme linked immunosorbent assay (ELISA) technology, the chemiluminescence immunoassay keeps high specificity of the ELISA technology, stability and reliability of a detection result, and convenience of operation, and can improve detection sensitivity simultaneously.

Description

technical field [0001] The invention belongs to the technical field of in vitro clinical testing and chemiluminescence immunoassay, and in particular relates to a chemiluminescence quantitative detection kit for sugar chain antigen 242 (CA 242) and related preparation methods and usage methods. Background technique [0002] Carbohydrate antigen 242 (carbohydrate antigen 242, CA242) was screened by monoclonal antibody C242 in 1985. In 1983, Limdholm et al. immunized mice with colorectal and rectal cancer cells COLD205 to obtain a series of antibodies, and later discovered a series of antigens corresponding to these antibodies, including CA 19-9, CA 50, CA 125, and CA 242. The determinants of this series of antigens are all sugar chain structures, and appear on the surface of the same mucin, but have different tumor specificities, so they can be used as different tumor markers. CA242 is a sialylated glycoprotein, the serum content of healthy people and benign diseases is very...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/545G01N33/574G01N21/76
Inventor 穆海东汪宁梅穆宇豪王通
Owner 上海裕隆生物科技有限公司
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