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Low-temperature aniline-degrading strain

An aniline-degrading bacteria and low-temperature technology, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., to achieve the effect of reducing processing costs, high application value, and broad application prospects

Inactive Publication Date: 2011-06-15
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
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  • Application Information

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Problems solved by technology

Domestic research on low-temperature aniline-degrading microorganisms is rare. For this, screening high-efficiency aniline-degrading bacteria under low-temperature conditions and understanding their degradation characteristics can provide microbial agent resources for the industrial treatment of low-temperature aniline wastewater, thereby solving the problem of low-temperature aniline. Laying the groundwork for problems with biological treatment systems for aniline wastewater

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specific Embodiment approach 1

[0011] Specific embodiment one: the low-temperature aniline-degrading bacterium of this embodiment, it is the low-temperature aniline-degrading bacterium A1, belongs to the new species of Pseudomonas (Pseudomonas), has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No.5011, the preservation date is December 01, 2010; it is a Gram-positive aerobic bacterium, rod-shaped, 3.0 μm in length and 0.8 μm in width; it forms white colonies on beef extract peptone medium, round Shaped, translucent, smooth surface, wavy edges, not easy to provoke.

[0012] In this embodiment, the low-temperature aniline-degrading bacteria A1 is routinely identified physiologically and biochemically on the low-temperature aniline-degrading bacteria A1 according to the "Bergey's Bacteriological Identification Manual". The experimental results are: positive contact enzyme reaction, positive indole produc...

specific Embodiment approach 2

[0020] Embodiment 2: This embodiment differs from Embodiment 1 in that the low-temperature aniline-degrading bacteria A1 has a positive contact enzyme reaction, a positive indole-producing test, a positive ammonia production test, a negative methyl red test reaction, negative starch hydrolysis, and oil hydrolysis. Positive, acetyl methyl alcohol test positive, nitrate reduction reaction negative, gelatin liquefaction positive, sulfuration test negative, litmus milk test peptonization, citric acid utilization positive, able to utilize glucose, unable to oxidize ethanol or aldose, growth temperature is 5~35℃, growth pH value is 5.5~9.5. Others are the same as in the first embodiment.

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Abstract

The invention relates to an aniline-degrading strain, in particular relating to a low-temperature aniline-degrading strain. The low-temperature aniline-degrading strain A1 with the collection number CGMCC No. 5011) belongs to Pseudomonas and was collected in China General Microbiological Culture Collection (CGMCC) on December 1st, 2010. The low-temperature aniline-degrading strain A1 provided by the invention is used for biological enhanced treatment of aniline-containing waste water in low-temperature conditions with 100% aniline degradation rate, no secondary environmental pollution and no need of addition of other chemicals, so that the waste water treatment cost is greatly reduced, and the application prospect is wide. The low-temperature aniline-degrading strain A1 is favorable for solving the problem of aniline-containing waste water biological treatment system in low-temperature conditions, and has high value of application.

Description

technical field [0001] The invention relates to an aniline degrading bacteria. Background technique [0002] Aniline is an important chemical raw material, mainly used as a medicine and rubber vulcanization accelerator, and is also a raw material for the manufacture of resins and coatings. Aniline toxic pollutants are highly toxic and difficult to degrade, causing serious pollution to the ecological environment. Therefore, studying the biodegradation of aniline compounds is of great significance for protecting the environment and improving human health. [0003] Low-temperature aniline-containing wastewater is one of the most difficult industrial wastewaters to treat. Domestic research on low-temperature aniline-degrading microorganisms is rare. For this, screening high-efficiency aniline-degrading bacteria under low-temperature conditions and understanding their degradation characteristics can provide microbial agent resources for the industrial treatment of low-temperatur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/38
Inventor 王继华马放崔迪刘翔李晶广慧牛佳关健飞
Owner HARBIN NORMAL UNIVERSITY
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