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Method for extracting corosolic acid from loguat leaf

A corosolic acid and extraction method technology, which is applied in the field of corosolic acid extraction, can solve the problems of high cost and cumbersome process, and achieve the effects of increasing yield, good dissolution effect and reducing consumption

Inactive Publication Date: 2011-06-15
天医堂(厦门)生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the disadvantages of cumbersome process and high cost in the corosolic acid extraction method in the prior art, the purpose of the present invention is to provide a method for extracting corosolic acid from loquat leaves with simple process, low production cost and high product purity

Method used

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  • Method for extracting corosolic acid from loguat leaf

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Comparison scheme
Effect test

Embodiment 1

[0024] 1) After the loquat leaf raw material is pulverized, the mass ratio of 8 times of the dry weight of loquat leaf is 80% ethanol aqueous solution ultrasonic extraction 2 times, the ultrasonic power is 220 watts, the working time is 40 minutes, the working temperature is 60 ℃, and the ultrasonic working mode is Work for 5 seconds, rest for 5 seconds, combine the extracts, filter the extracts, and concentrate them in a vacuum to form a concentrated solution;

[0025] 2) Add 8 times the volume of the concentrated solution and acid water with a pH value of 5-6 to the concentrated solution, let it sit and filter to obtain a filter residue;

[0026] 3) Add mass ratio to filter residue and be 40%, PH=11 aqueous ethanol solution is fully dissolved, obtains mixed solution, and mixed solution is added above-mentioned ethanol aqueous solution, adjusts the content of corosolic acid in mixed solution to be about 1.5g / L;

[0027] 4) The macroporous resin adsorbs the corosolic acid mixt...

Embodiment 2

[0030] 1) After the loquat leaf raw material is pulverized, the mass ratio of 10 times of the dry weight of loquat leaf is 75% ethanol aqueous solution ultrasonic extraction 2 times, the ultrasonic power is 250 watts, the working time is 20 minutes, the working temperature is 40 ℃, and the ultrasonic working mode is Work for 5 seconds, rest for 5 seconds, combine the extracts, filter the extracts, and concentrate them in a vacuum to form a concentrated solution;

[0031] 2) Add 10 times the volume of the concentrated solution and acid water with a pH value of 5-6 to the concentrated solution, let it stand and filter to obtain a filter residue;

[0032] 3) add mass ratio to the filter residue and be 50%, PH=9 aqueous ethanol solution is fully dissolved, obtains the mixed solution, the mixed solution is added above-mentioned ethanol aqueous solution, makes the content of corosolic acid in the mixed solution be about 1.5g / L;

[0033] 4) The macroporous resin adsorbs the corosolic...

Embodiment 3

[0036] 1) After the loquat leaf raw material is pulverized, use 12 times the mass ratio of loquat leaf dry weight as 85% ethanol aqueous solution ultrasonic extraction 2 times, the ultrasonic power is 180 watts, the working time is 30 minutes, the working temperature is 50 ℃, and the ultrasonic working mode is Work for 5 seconds, rest for 5 seconds, combine the extracts, filter the extracts, and concentrate them in a vacuum to form a concentrated solution;

[0037] 2) Add 12 times the volume of the concentrated solution and acid water with a pH value of 5-6 to the concentrated solution, let it stand and filter to obtain a filter residue;

[0038] 3) add mass ratio in filter residue and be 45%, the ethanol aqueous solution of PH=10 is fully dissolved, obtains mixed solution, and mixed solution is used to add above-mentioned ethanol aqueous solution, makes the content of corosolic acid in corosolic acid mixed solution be 1.5g / About L;

[0039] 4) The macroporous resin adsorbs ...

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Abstract

The invention discloses a method for extracting corosolic acid from loguat leaf. Based on the loguat leaf as a raw material, the high pure corosolic acid is obtained by combination of ultrasonic extraction, acid precipitation, alkaline alcohol solution dissolution, macroporous resin absorption, crystallization, re-crystallization and the like. The method disclosed by the invention has the advantages of simple process, low production, high extraction efficiency and the like.

Description

technical field [0001] The invention relates to a plant extraction method, in particular to a method for extracting corosolic acid from loquat leaves. Background technique [0002] Corosolic acid, also known as 2α-hydroxyursolic acid, is a triterpenoid compound present in plants such as Lagerstroemia indica and loquat. [0003] [0004] Schematic diagram of the chemical structure of corosolic acid [0005] Coro carboxylic acid is a white amorphous powder, soluble in petroleum ether, benzene, chloroform and other organic solvents, insoluble in water, soluble in hot ethanol and methanol. Under anhydrous conditions, it will react with strong acid (sulfuric acid, phosphoric acid, perchloric acid), medium strong acid (trichloroacetic acid) or Lewis acid (zinc chloride, aluminum trichloride, antimony trichloride) or fluorescence. Corosolic acid belongs to α-amyresinol type or ursane type pentacyclic triterpenoids, and its basic skeleton is the pentacyclic core of polyhydropi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 郑志忠蒋荔馨陈义甘全明林志华
Owner 天医堂(厦门)生物工程有限公司
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