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Large-scale process for the preparation of thylakoids

A thylakoid, large-scale technology, applied in the field of large-scale preparation of thylakoids, can solve problems such as should not be degraded

Inactive Publication Date: 2011-06-08
THYLABISCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, being able to produce thylakoids on a large scale faces a number of problems, including maintaining the integrity of the proteins, i.e. they should not degrade

Method used

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  • Large-scale process for the preparation of thylakoids
  • Large-scale process for the preparation of thylakoids
  • Large-scale process for the preparation of thylakoids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Production of thylakoids from spinach

[0084] About 1000 kg of starting material are required to produce 30 kg of dried thylakoid membranes from spinach.

[0085] Spinach with dry content: 8-9%

[0086] About 0.01%-0.03% (w / w) dried thylakoid membrane fraction was obtained from spinach fresh weight.

[0087] 1. Mix 1000 kg of frozen spinach with drinking water 1:1 using a screw press and a mixer. The amount of dry matter is about 85 kg.

[0088] 2. Add EDTA to a concentration of 10 mM.

[0089] 3. Dilute the homogenate / serum until the final ratio of spinach to drinking water is 1:10 and the volume is 10m 3 . Pass the slurry through a decanter to remove cell debris from the liquid.

[0090] 4. Separation of the thylakoid fraction with an alfa laval separator. About 600 liters of a thick liquid of about 5% dry matter are obtained.

[0091] 5. After freezing for 24 hours, thawing, and filtering through a 5 μm filter, the thylakoid fraction was concentrated 5-fold ...

Embodiment 2

[0094] Product Effects on Insulin

[0095] Insulin has important anabolic effects, causing binge eating and obesity. It is therefore important to achieve lowering of insulin levels in various dietary regimens. To investigate whether thylakoids affect insulin secretion, insulin secretion was measured during consumption of a control diet and a diet containing thylakoids. The study was performed in humans. Eleven healthy subjects fasted overnight and were then given either a control diet or a thylakoid-rich diet, with a one-week interval between each diet to ensure clearance. The control diet consisted of bread, tomato, pesto with rapeseed oil, and cashew nuts with basil, while the thylakoid diets (4 different experimental diets) contained the same components, supplemented with different doses of thylakoids Powder (50g, 25g, 10g or 5g). Blood samples were taken at various time points of 0, 30 minutes, 1 hour, 2 hours, 4 hours and 6 hours. Glucose and insulin levels were meas...

Embodiment 3

[0097] Product Effect on Body Fat

[0098] To study the effect of thylakoids on body fat, mice were fed a high-fat diet or a thylakoid-enriched high-fat diet for 100 days. Thirty female apoE-deficient mice were obtained from Taconic (Taconic Europe, Lille Skensved, Denmark) and divided into two groups (n=15 per group); one group received cholesterol containing 0.15% (by energy) and 43% (by energy) energy meter) a high-fat diet of saturated fat (control group; R638, Lactamin, Kimsta, Sweden), and another group received a high-fat diet rich in thylakoids (R638) at a dose of 6 mg chlorophyll / g diet. The control diet and the thylakoid-enriched diet were isocaloric and had the same caloric composition between macronutrients. Three mice per cage were housed in a temperature-controlled environment (21° C.) with a 12-h light / dark cycle (lights on at 6 am). Mice had free access to their respective diets and tap water at all times. Food intake was measured every other day and body we...

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Abstract

The present invention relates to a process on a large industrial scale for the production of thylakoids, from photosynthetic organisms, such as from green plant leaf material, to be used as ingredients in food, or additions to food, or dietary supplements, or pharmaceuticals for the purpose of preventing overweight, promoting satiety, reducing food intake, reducing bodyweight, reducing blood insulin concentration and reducing blood fats and percentage body fat in humans and animals.

Description

field of invention [0001] The present invention relates to a large industrial scale process for the production of thylakoids from photosynthetic organisms, such as green plant leaf material, for use as ingredients in food, additions to food, food additives Drugs, or drugs for the purpose of preventing overweight, promoting satiety, reducing food intake, reducing body weight, reducing blood insulin concentrations, reducing blood lipids and percent body fat in humans and animals. Background technique [0002] Overweight and obesity have become a worldwide problem. As part of the metabolic syndrome, obesity can lead to diseases such as diabetes, arteriosclerosis and high blood pressure. Obesity and overweight are the result of an imbalance between energy intake and energy expenditure, usually caused by a lack of appetite control. A high-fat diet alone or in combination with sucrose and other sweet carbohydrates is one of the most important contributors to obesity because thes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/00A61K36/18A61P3/04A61K127/00
CPCA61K36/288A61K36/185A61K36/28A61K36/21A61K36/02A61P29/00A61P3/04A61P3/06A61P5/48A61K36/86
Inventor C·厄兰森-阿尔伯特森P-A·阿尔伯特森G·里留斯M·奥尔贝
Owner THYLABISCO
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