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Method for preparing Vero cell influenza virus vaccine

A technology for influenza virus and influenza vaccine, which is used in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc.

Active Publication Date: 2011-06-01
吉林亚泰生物药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Purification of traditional viral vaccines using hydrophobic chromatography methods has not been reported in the literature

Method used

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  • Method for preparing Vero cell influenza virus vaccine
  • Method for preparing Vero cell influenza virus vaccine
  • Method for preparing Vero cell influenza virus vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Type A virus strain, approved by Who, Solomon strain, A / Solomon / 3 / 06 (H1N1)-, IVR-145, from NIBSC, UK, batch number: Ivr-145, 06 / 234, generation E 7 (Chicken embryo passages are represented by E and numbers, cells are represented by C and numbers) Vero monolayer cells, passage 140-145.

[0038] The A / Solomon / 3 / 06 (H1N1) E7C0 virus seed derived from NIBSC was used to infect Vero cells and chicken embryos for passage, and the passage of the virus seed to E 8 C 0 , the hemagglutination titer was 640, and the passage of Vero cells was E 7 C 1 , The hemagglutination titer is 20-40. E 8 C 0 The Vero cells were infected with the virus species and continued to be subcultured, harvested when the cytopathic changes reached +++-++++, and the main generation seed batch (hemagglutination titer 320) and working seed batch (hemagglutination titer 480) were established and used for Preparation of monovalent vaccines.

[0039]225 bottles of monolayer Vero cells 3 liter spi...

Embodiment 2

[0068] A type 3 virus, A / wisconsin / 67 / 2005 (H3N2)-, NYMC-161, from NIBSC, batch number: 06 / 112, generation E 9

[0069] SPF eggs: Beijing Meria Verton Laboratory Animal Technology Co., Ltd.

[0070] Incubate in a biochemical incubator at 37°C for 10 days.

[0071] Vero monolayer cells, passage 140-145;

[0072] A / wisconsin / 67 / 2005 (H3N2)E 9 C 0 Infected chicken embryos derived from NIBSC virus species for passage, the passage of the virus species after passage of chicken embryos is E 10 C 0 , the hemagglutination titer is 480, the virus seed infects Vero cells and continues to be subcultured, and the cell lesion reaches +++-++++ and harvests, and establishes the main generation seed batch (hemagglutination titer 240) and the working seed batch (blood Clotting titer 480), and used in the preparation of monovalent vaccines.

[0073] 225 bottles of monolayer Vero cells 3 liter spinner bottle, cell passage 145, 5 days old, wash the cells three times with washing s...

Embodiment 3

[0101] Type B virus, B / Malaysia / 2506 / 2004, from NIBSC, UK, batch number: 06 / 104, generation E 4

[0102] SPF eggs: Beijing Meria Verton Laboratory Animal Technology Co., Ltd.

[0103] Incubate in a biochemical incubator at 37°C for 10 days.

[0104] Vero monolayer cells, passage 140-145;

[0105] Will B / Malaysia / 2506 / 2004E 4 C 0 Originated from NIBSC virus species infected chicken embryos and Vero cells, the hemagglutination titer reached 240, and the virus generation after passage was E 5 C 0 , while Vero cells were passaged to E 4 C 1 , with a hemagglutination titer of 10. Will B / Malaysia / 2506 / 2004E 4 C 1 Virus seeds are returned to chicken embryos, and the hemagglutination titer can reach 480, and then continue to be passed on Vero cells to establish the main generation seed batch (hemagglutination titer 160) and working seed batch (hemagglutination titer 240), which are used for monovalent vaccines preparation.

[0106] 225 bottles of monolayer Vero ...

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Abstract

The invention provides a method for preparing a Vero cell influenza virus vaccine. Seasonal trivalent influenza vaccines and pandemic influenza storage vaccines with qualified quality can be prepared by improving a vaccine purification process, combining hydrophobic chromatography with ion exchange and auxiliarily adopting other purification methods.

Description

technical field [0001] The invention provides a preparation method of Vero cell influenza virus vaccine, relates to the preparation method of various influenza virus vaccine strains on Vero cells, and belongs to the field of biotechnology. Background technique [0002] The traditional method of preparing influenza vaccine is to use chicken embryos, which has a history of more than 50 years in China. The currently used influenza vaccines are mainly inactivated influenza trivalent split vaccines prepared from chicken embryos, including influenza A strains, H3N2 and influenza B strains, and large influenza vaccines (including H5N1, etc.). The source of the vaccine strain is mainly determined by the surface antigen of the influenza virus epidemic strain recommended by the WHO according to the annual change of the global influenza virus for the next year's influenza vaccine production. Strain (A / PR / 8 / 34 (H1N1)) double infection produces reassortment or reverse genetic reassortme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/145A61K39/295A61P31/16
Inventor 于洪涛李光谱张莹周庆玲岳振齐董唤胡晓明李淑兰郭岩
Owner 吉林亚泰生物药业股份有限公司
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