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Ganoderma sinensis mycelium anti-tumor polysaccharide component GS-C as well as preparation method and application thereof

A GS-C, anti-tumor technology, used in anti-tumor drugs, medical preparations containing active ingredients, drug combinations, etc., can solve problems such as molecular weight and purity of polysaccharides that are not involved, reduce the molecular weight range and increase polysaccharide content. , the effect of high purity

Inactive Publication Date: 2011-05-04
SHANGHAI INST OF PHARMA IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of the above patents and articles involve research on the molecular weight and purity of polysaccharides

Method used

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  • Ganoderma sinensis mycelium anti-tumor polysaccharide component GS-C as well as preparation method and application thereof
  • Ganoderma sinensis mycelium anti-tumor polysaccharide component GS-C as well as preparation method and application thereof
  • Ganoderma sinensis mycelium anti-tumor polysaccharide component GS-C as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Preparation of crude polysaccharide from Zizhi mycelium:

[0055] Zizhi liquid submerged fermentation liquid 1300L (the Zizhi liquid submerged fermentation liquid is obtained by the method described in Chinese patent 200710045369.4 "A Zizhi fermentation method and prepared Zizhi mycelium"), the mycelium is extracted with water for 1-3 hours, The extract was concentrated under reduced pressure, ethanol was added for precipitation, and the precipitated part was dried to constant weight to obtain 150 g of crude polysaccharide.

[0056] Ultrafiltration separation:

[0057] Dissolve 150g of the extract in 20 times deionized water, and ultrafilter it with a polyamide hollow cellulose ultrafiltration membrane with a molecular weight cut off of 10,000. The pressure is 0.1-0.3mPa. Preferably, collect the retentate, concentrate the liquid with a nanofiltration membrane with a molecular weight cut-off of 150, and freeze-dry to obtain 62.3 g of polysaccharides greater than 10,000 ...

Embodiment 2

[0062] Zizhi liquid submerged fermentation liquid 1300L (the Zizhi liquid submerged fermentation liquid is obtained by the method described in Chinese patent 200710045369.4 "A Zizhi fermentation method and prepared Zizhi mycelium"), the mycelium is extracted with water for 1-3 hours, The extract was concentrated under reduced pressure, ethanol was added for precipitation, and the precipitated part was dried to constant weight to obtain 150 g of crude polysaccharide.

[0063] Ultrafiltration separation:

[0064] Dissolve 150g of the extract in 30 times deionized water, and ultrafilter it with a polyamide hollow cellulose ultrafiltration membrane with a molecular weight cut-off of 30,000. The pressure is 0.1-0.3mPa, and the solution flow rate depends on the membrane to maintain good permeability Preferably, the retentate is collected, the liquid is concentrated with a nanofiltration membrane with a molecular weight cut-off of 200, and freeze-dried to obtain 60 g of polysaccharid...

Embodiment 3

[0069] Zizhi liquid submerged fermentation liquid 1300L (the Zizhi liquid submerged fermentation liquid is obtained by the method described in Chinese patent 200710045369.4 "A Zizhi fermentation method and prepared Zizhi mycelium"), the mycelium is extracted with water for 1-3 hours, The extract was concentrated under reduced pressure, ethanol was added for precipitation, and the precipitated part was dried to constant weight to obtain 150 g of crude polysaccharide.

[0070] Ultrafiltration separation:

[0071] Dissolve 150g of the extract in 20 times deionized water, and use a tubular ultrafiltration membrane made of sulfonated polysulfone with a molecular weight cut-off of 30,000 for ultrafiltration. The pressure is 0.1-0.3mPa. The rate is appropriate, collect the retentate, concentrate the liquid with a nanofiltration membrane with a molecular weight cut-off of 250, and freeze-dry to obtain 56.8 g of polysaccharides greater than 30,000 in the mycelia of Zizhi mycelium.

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Abstract

The invention provides a ganoderma sinensis mycelium anti-tumor polysaccharide component GS-C, which contains polysaccharide and monosaccharide and is characterized in that the content of the polysaccharide is 85-90 weight percent. The invention also provides a preparation method and application of the ganoderma sinensis mycelium anti-tumor polysaccharide component GS-C. The ganoderma sinensis mycelium anti-tumor polysaccharide GS-C has the advantages of higher polysaccharide content, lower medication dose, concentrated molecular weight range and higher purity, and the preparation process is suitable for industrial production.

Description

technical field [0001] The invention relates to the anti-tumor polysaccharide component GS-C of Zizhi mycelium, its preparation method and application. Background technique [0002] Edible and medicinal fungi have various functions such as anti-tumor and anti-virus, lowering blood lipids, delaying aging, protecting the liver and detoxifying, promoting nucleic acid and protein biosynthesis, etc., and have a long history of use in China. A large number of studies at home and abroad have shown that fungal polysaccharides are isolated from fungal fruiting bodies, mycelia, and fermentation broths. They are the active parts of fungal drugs, and they are a type of active polysaccharides that can control cell division and differentiation, and regulate cell growth and aging. . [0003] Ganoderma sinense (Ganoderma sinense) is a fungus of the genus Ganoderma (2005 Chinese Pharmacopoeia), which has obvious anti-tumor and immunity-enhancing effects. Due to the unclear preparation proc...

Claims

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Application Information

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IPC IPC(8): C08L5/00A61K31/715A61P35/00A61P37/04
Inventor 杨义芳金隽迪杨国红
Owner SHANGHAI INST OF PHARMA IND
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