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Fenvalerate pesticide detection method

A technology of fenvalerate and a detection method, which is applied in the direction of measuring devices, instruments, and material analysis by electromagnetic means, can solve the problems of high cost and time-consuming antigen and antibody, and achieve low cost, simple and easy preparation steps, time-saving effect

Inactive Publication Date: 2011-04-27
SHENZHEN POLYTECHNIC
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Problems solved by technology

The main disadvantages of the immunization method are: the preparation process of antigen and antibody is time-consuming and costly

Method used

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  • Fenvalerate pesticide detection method
  • Fenvalerate pesticide detection method

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Embodiment Construction

[0021] The present invention firstly provides a method for detecting fenvalerate pesticide, which is specifically implemented by using a DNA electrochemical biosensor.

[0022] In specific applications, fenvalerate will undergo electrochemical behavior on DNA electrochemical biosensors, such as figure 1 Shown, the relationship between the DPV peak current of fenvalerate and its concentration, in 9.5×10 -8 ~9.5×10 -6 There is a linear relationship within the mol / L concentration range, and the linear regression equation is: According to the measured DPV peak current of fenvalerate, the concentration of fenvalerate can be further determined. It should be noted that the above linear regression equation is obtained by using the prepared DNA electrochemical biosensor to measure multiple sets of current and concentration data through pre-experimental experiments. The linear regression equation is affected by many factors in the preparation process of DNA electrochemical biosenso...

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Abstract

The invention relates to a fenvalerate pesticide detection method. A prefabricated DNA electrochemical biosensor is used for detecting an object to be detected and comprises a carbon fiber electrode serving as a substrate, wherein the surface of the carbon fiber electrode is successively overlaid and provided with a peroxide polypyrrole film, a nanogold layer and a DNA layer from inside to outside; the fenvalerate generates electrochemistry behaviour on the DNA electrochemical biosensor; the DPV (differential pulse voltammetry) peak current value of the fenvalerate is measured to determine the density of the fenvalerate. The invention utilizes the DNA electrochemical biosensor to detect fenvalerate pesticide, utilizes the electrochemical detection mode, and can save complex pretreatment works. The instrument is simple, has low cost and realizes on-field detection; in addition, disposable carbon fiber modified electrodes are used. The DNA electrochemical biosensor has the advantage of simple and easy preparation steps, and the electrode can be modified in advance, thus realizing batch detection and saving time.

Description

technical field [0001] The invention relates to the technical field of pesticide detection, in particular to a method for detecting fenvalerate pesticide. Background technique [0002] Pyrethroid pesticides are widely used, and fenvalerate is one of the main ones. At present, the detection methods of fenvalerate pesticide residues are mainly chromatography and immunoassay. [0003] Wherein, the chromatographic method specifically includes the following steps: 1. preparation of pesticide standard samples; 2. sample preparation; 3. sample extraction and purification, wherein the extraction process is as follows: take 25.0 g of crushed samples and add petroleum ether: Acetone = 1:1 solution 100mL, placed in a tissue masher for homogenate extraction for 2min, vacuum filtration, washed filter residue with 25mL mixed solvent respectively, the filtered solution was extracted with an equal volume of saturated sodium sulfate solution into a separatory funnel, vigorously Shake for 1...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327G01N27/60
Inventor 栾崇林刘引吴朝阳蒋晓华
Owner SHENZHEN POLYTECHNIC
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