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Detection method of Chinese patent medicines containing at least two kinds of Radix Paeoniae Alba, Ginseng, Danshen, Artemisia annua, Licorice, Chuanxiong and Angelica

A detection method and technology of Chinese patent medicine, applied in the field of detection of active ingredients of traditional Chinese medicine, can solve the problems of wide range of medicinal materials, internal quality of difficult Chinese medicine pills, complex chemical components, etc., and achieve objective and accurate identification results, accurate and stable results, The effect of eliminating noise peaks

Inactive Publication Date: 2011-03-30
NAT INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Many Chinese patent medicines are composed of a variety of medicinal flavors. For example, Tongren Wuji Baifeng Pills are composed of nineteen medicinal materials. The medicinal materials involve a wide range of ingredients and complex chemical components. It is difficult to fully control the quality of only a dozen active ingredients. The inherent quality of Chinese patent medicine pills, let alone its authenticity

Method used

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  • Detection method of Chinese patent medicines containing at least two kinds of Radix Paeoniae Alba, Ginseng, Danshen, Artemisia annua, Licorice, Chuanxiong and Angelica
  • Detection method of Chinese patent medicines containing at least two kinds of Radix Paeoniae Alba, Ginseng, Danshen, Artemisia annua, Licorice, Chuanxiong and Angelica
  • Detection method of Chinese patent medicines containing at least two kinds of Radix Paeoniae Alba, Ginseng, Danshen, Artemisia annua, Licorice, Chuanxiong and Angelica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1. The quality detection method of Tongren Wuji Baifeng Pills (Darmi Pills, Tongrentang Pharmaceutical)

[0083] Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; acetonitrile is used as mobile phase A, water is used as mobile phase B, and the gradient elution is carried out in the following table; the detection wavelength is 203nm (white peony , ginseng, licorice, Artemisia annua medicinal materials), 280nm (salvia miltiorrhiza, Chuanxiong, angelica medicinal materials).

[0084] Table HPLC mobile phase gradient

[0085]

[0086] Preparation of the test solution: Take an appropriate amount of this product, cut it into pieces, add an equal amount of diatomaceous earth and grind it evenly, accurately weigh 12g, add methanol 50mL, ultrasonically treat (power 300W, frequency 50kHz) for 30 minutes, let cool, and filter , the filtrate recovered the solvent and concentrated to dryness, the residue was diss...

Embodiment 2

[0092] Example 2. The detection method of active ingredients in Wuji Baifeng Pills (Shuiwan, Huiren Pharmaceutical)

[0093] 1. Preparation of the test solution: Accurately weigh Wuji Baifeng Pills (water pills 6g), add methanol equivalent to about 8 times the amount of the test sample, dissolve, preferably ultrasonically treat for 20-40 minutes, filter, and recover the solvent from the filtrate Concentrate to dryness, add about 50mL water to the residue to dissolve, pass through D101 macroporous resin adsorption column (the resin packed in the column is about 60g, inner diameter 2cm, length 12cm), elute with about 300mL water, discard the water, and use 25-35%wt of 50mL of ethanol for elution, discard the eluent, then use 75-85%wt (preferably 80%wt) of about 200mL of ethanol for elution, collect the eluent, evaporate to dryness, and wash the residue with methanol Dissolve and transfer to a measuring bottle (preferably a 5mL measuring bottle), add methanol to the mark, pass th...

Embodiment 3

[0100] Example 3. Detection method of active ingredients in other traditional Chinese medicine preparations containing two or more medicinal ingredients from Radix Paeoniae Alba, Ginseng, Salvia, Artemisia annua, Radix Glycyrrhizae, Rhizoma Chuanxiong, Angelica

[0101] 1. Preparation of the test solution: Accurately weigh the test product (should contain Radix Paeoniae Alba, Ginseng, Salvia miltiorrhiza, 6g), add methanol equivalent to about 8 times the amount of the test product, dissolve, filter , the filtrate is recovered and the solvent is concentrated to dryness, the residue is dissolved by adding about 50mL of water, passed through a D101 type macroporous resin adsorption column (about 60g of resin packed in the column), eluting with about 300mL of water, discarding the water, and then using 25- Elute with 50 mL of ethanol at 35% wt, discard the eluate, and then elute with about 200 mL of ethanol at 75% wt, collect the eluate, evaporate to dryness, dissolve the residue w...

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Abstract

The invention provides a method for detecting a Chinese patent drug containing at least two of white paeony root, ginseng, salvia miltiorrhiza, sweet wormwood, liquorice and angelica sinensis, comprising the steps as follows: preparing a sample solution and a comparison product drug solution, adopting acetonitrile and water as mobile phases, and performing high performance liquid chromatography (HPLC) measurement, wherein the detection wavelength is 201-290nm; and detecting whether a corresponding chromatographic peak exists at a position, which has the same reservation time with the chromatograph of a comparison drug, in the chromatography of a sample. Compared with the traditional thin-layer chromatography identification method, the detection method in the invention has the advantages that the mode of processing a sample containing various drugs in a liquid phase chromatograph is changed, the mixed peak in the liquid phase chromatography except the components to be detected in the Chinese patent drug is successfully eliminated, and results are more accurate and stable.

Description

technical field [0001] The invention provides a method for detecting active ingredients in medicines, which is a method for detecting active ingredients in traditional Chinese medicines, especially refers to containing two or more of Radix Paeoniae Alba, Ginseng, Salvia Miltiorrhiza, Artemisia annua, Licorice, Chuanxiong, and Angelica. The detection method of active ingredients in traditional Chinese medicine preparations with more than one medicinal ingredients. Background technique [0002] Traditional Chinese medicine compound prescription is the main form and method of TCM clinical medicine, and each medicine in the prescription contains various chemical components. These inherent chemical components are the material basis for the compound prescription to exert its medicinal effect, but any active ingredient in the prescription cannot fully reflect The overall curative effect embodied by traditional Chinese medicine is the fundamental difference between traditional Chine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61K36/258A61K36/537A61K36/282A61K36/484A61K36/236A61K36/232A61K36/8965A61P15/00G01N30/02G01N30/28A61K35/64A61K35/56A61K35/32A61K35/12A61K35/57A61K35/586A61K35/618A61K35/644
Inventor 林瑞超王钢力聂黎行李志猛陈佳范国强
Owner NAT INST FOR FOOD & DRUG CONTROL
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