High-throughput STR typing system capable of identifying complex consanguinity and kit thereof
A kit, the technology of D1S1600, is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve problems such as inability to distinguish complex genetic relationships
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Embodiment 1
[0078] Example 1: A STR typing system and its kit for complex genetic relationship identification
[0079] 1.1 Composition and characteristics of autosomal-linked STR loci:
[0080] (1) The STR typing system in this embodiment comprises the following 60 STR loci:
[0081] D1S2131、D1S3721、D1S2130、D1S1600、D1S1653、D1S1660、D1S3732、D2S1364、D2S2734、D2S1396、D2S428、D2S435、D2S1387、D2S1792、D2S1399、D2S2959、D3S2431、D3S4547、D4S1643、D4S2408、D4S3326、D4S2368、D5S2845、D5S1473、D5S813、 D5S1716、D5S1459、D5S1487、D5S1466、D5S2496、D5S2501、D6S1019、D6S2417、D6S2412、D6S1284、D7S1820、D7S3050、D7S821、D8S594、D8S1468、D8S2322、D8S569、D8S1475、D9S746、D9S319、D9S2149、D11S2010、D11S1392、D12S376、D12S1052、 D13S317, D13S790, D14S1432, D14S1428, D15S644, D15S1507, D16S752, D16S485, D20S481 and D20S1151;
[0082] It has the following measurable properties:
[0083] 1) The repeating unit of the core region is tetranucleotide;
[0084] 2) The heterozygosity of each STR is greater than 0.6;
[0085] 3) Each group contains ...
Embodiment 2
[0105] Example 2: A STR typing system and its kit for complex genetic relationship identification
[0106] 2.1 Composition and characteristics of autosomal-linked STR loci:
[0107] (1) The STR typing system in this embodiment comprises the following 58 STR loci:
[0108]D1S3736、D1S1665、D1S2127、D1S2138、D1S1642、D1S518、D1S1604、D1S3729、D1S3727、D2S1336、D2S1779、D2S1771、D2S437、D2S2970、D2S2944、D2S1327、D3S2402、D3S1766、D4S2411、D4S3351、D4S243、D4S2426、D4S2373、D5S1490、D5S2856、 D5S2495、D5S2855、D5S1722、D5S1725、D6S1048、D6S1275、D7S796、D7S1799、D8S2324、D8S2330、D8S1144、D9S745、D9S301、D9S1124、D10S1246、D10S2485、D11S1983、D11S2363、D11S1368、D12S393、D12S1063、D13S1807、D13S1492、D14S738、D14S301、 D14S583, D15S816, D15S1514, D18S872, D18S972, D18S548, D20S1145, D20S477;
[0109] It has the following measurable properties:
[0110] 1) The repeating unit of the core region is tetranucleotide;
[0111] 2) The heterozygosity of each STR is greater than 0.6;
[0112] 3) Each group contains at least two STRs...
Embodiment 3
[0125] Example 3: A STR typing system and its kit for complex genetic relationship identification
[0126] 3.1 Composition and characteristics of autosomal-linked STR loci:
[0127] (1) The STR typing system in this embodiment comprises the following 61 STR loci:
[0128] D1S532、D1S1611、D1S3733、D1S533、D1S1614、D2S2977、D2S1374、D2S1394、D2S2966、D2S2969、D2S1371、D2S434、D2S1338、D3S4016、D3S2388、D4S1626、D4S1653、D5S2858、D5S2796、D5S1463、D5S815、D5S2499、D5S2498、D6S1043、D6S1274、 D6S1056、D6S1013、D6S1054、D7S820、D7S2205、D7S3071、D7S2845、D8S2326、D8S1464、D8S2320、D8S1470、D8S588、D8S1471、D9S2026、D9S747、D9S2128、D10S2469、D10S1238、D11S4464、D11S4958、D13S1818、D13S767、D14S615、D14S608、D14S597、 D14S302, D14S749, D16S767, D16S3393, D18S537, D18S875, D18S1367, D20S1152, D20S206, D20S607, D20S1146;
[0129] It has the following measurable properties:
[0130] 1) The repeating unit of the core region is tetranucleotide;
[0131] 2) The heterozygosity of each STR is greater than 0.6;
[0132] 3) Each group ...
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