Method and PCR (polymerase chain reaction) primer for identifying dendrobium officinale

Abstract

The invention relates to a method and a PCR (polymerase chain reaction) primer for identifying dendrobium officinale. The method includes identifying the dendrobium officinale on the basis of mononucleotide difference sites of chloroplast ACCD gene sequences of the dendrobium officinale and other herba dendrobii of dendrobium by the aid of Tetra-primer ARMS-PCR processes. The PCR primer for identifying the dendrobium officinale comprises specific inner primers for identifying the dendrobium officinale and the other herba dendrobii of the dendrobium and a pair of specific outer primers. The method is simple, and the dendrobium officinale can be detected by the aid of one-step PCR and conventional electrophoretic techniques; identification results are accurate, mismatched basic groups are introduced when the primers are designed, accordingly, the specificity of PCR amplification reaction can be greatly enhanced, and false negative results can be prevented owing to positive control of outer primer strips; further, the method is sensitive, and the dendrobium officinale can be completely identified only by a few samples.

Description

technical field [0001] The invention relates to a method for identifying dendrobium officinale based on a single nucleotide difference site in a chloroplast DNA sequence, in particular to a method for identifying dendrobium officinale and a PCR primer used therefor. Background technique [0002] Dendrobium officinale (Dendrobium officinale), also known as black knot grass, is a perennial epiphytic herb of Orchidaceae (Orchidaceae). They are mainly distributed in Guangxi, Yunnan, Zhejiang, Fujian, Anhui and other places in my country. Dendrobium candidum is known as "life-saving fairy grass" and "inch golden grass" in the folks. It has remarkable therapeutic and health effects. Because Dendrobium candidum is very rare, due to human over-excavation and serious habitat damage, coupled with the unique habitat of Dendrobium candidum and difficult reproduction, As early as 1987, Dendrobium officinale was included in the "List of National Key Protected Wild Medicinal Species". Due ...

AI Technical Summary

Problems solved by technology

Traditional identification based on traits and microscopic features is difficult to grasp and teach in practice due to the influence of the experience of the identifyrs, individual differences and other factors; The origin of Dendrobium raw materials, the environmental conditions for the growth of Dendrobium candidum, etc., the stability is poor

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