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Method for fusing protoplast of volvaria volvacea and pleurotus eryngii

A technology of protoplast fusion and protoplast, which is applied in the field of high-yield and low-temperature resistant Strain mushroom strains, can solve problems such as poor results, and achieve high yield, strong technical repeatability, and good genetic stability

Inactive Publication Date: 2011-03-16
SOUTH CHINA AGRI UNIV
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  • Method for fusing protoplast of volvaria volvacea and pleurotus eryngii
  • Method for fusing protoplast of volvaria volvacea and pleurotus eryngii
  • Method for fusing protoplast of volvaria volvacea and pleurotus eryngii

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Embodiment 1

[0039] (1) Inoculate the V138 strain on the PDSA plate according to the conventional method, and cultivate it in a constant temperature incubator at 32°C for 3 days as the straw mushroom strain; inoculate the Pleurotus eryngii strain on the PDSA plate according to the conventional method, and cultivate it at a constant temperature of 25°C Cultivate in box for 5-7 days as the strain of Pleurotus eryngii.

[0040] (2) Take 3 pieces of Straw mushroom strains with a diameter of 5 mm (1) in the ultra-clean bench, put them into a 100 mL Erlenmeyer flask containing 20 mL of PDSB liquid medium, and place them at a constant temperature of 33°C for static culture for 3 days. After hooking out the mycelium block with an inoculation needle, remove the internal bacterial seed block and rinse repeatedly with sterile water, then repeatedly rinse with 0.6mol / L osmotic pressure stabilizer to remove the residual medium, and then use sterile filter paper Absorb excess water to obtain fresh straw...

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Abstract

The invention provides a method for fusing protoplast of volvaria volvacea and pleurotus eryngii. The mycelium of the volvaria volvacea and pleurotus eryngii is in enzymolysis to prepare a protoplast; the thermal inactivation of the pleurotus eryngii protoplast serves as a mark, and a PEG (polyethylene glycol)-mediated chemical method is adopted to fuse the protoplast of volvaria volvacea and pleurotus eryngii; a fusant is screened at the temperature of 0 DEG C, and a fruiting experiment is carried out to obtain a fusion strain. The method provided by the invention can effectively fuse the protoplast of volvaria volvacea and pleurotus eryngii and obtain low temperature resistant new volvaria volvacea strain with the characteristics of pleurotus eryngii. The invention provides an effectivemethod for distant fusion creation between two edible fungusiaceae with obvious complementary character.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding of edible fungi, and in particular relates to a method for obtaining a high-yield and low-temperature-resistant straw mushroom strain with the characteristics of Pleurotus eryngii by fusion of straw mushroom and pleurotus eryngii protoplasts. Background technique [0002] Straw mushroom is a kind of edible fungus, which is rich in nutrition and delicious, and is deeply loved by consumers. Straw mushroom is a typical tropical high-temperature edible fungus, which belongs to Agaricaceae, Phyllosporaceae, and the genus Agaricus in classification. Straw mushroom hyphae have poor resistance to low temperature, and are very sensitive to changes in environmental conditions, especially temperature changes: when the average temperature is below 27°C, it is difficult for straw mushroom fruiting bodies to form. Changes in temperature, especially a sudden drop in temperature, are extremely unfavorable t...

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Application Information

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IPC IPC(8): C12N15/04
Inventor 郭丽琼游丽容林俊芳曾志忠
Owner SOUTH CHINA AGRI UNIV
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