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Plant growth bacterial agent and preparation method thereof

A plant growth and bacterial agent technology, applied in the direction of plant growth regulators, plant growth regulators, botany equipment and methods, etc., to achieve the effects of reducing raw material preparation, simple production process, and saving manpower

Inactive Publication Date: 2011-03-16
甘肃求实生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] There are a variety of microbial agents used for plant growth in China, such as Japan's EM, Shenli CM, VT, etc. , Trichoderma konshii complex preparation method of bacterial agent and the technology of bacterial agent that can help plants grow rapidly is still blank

Method used

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  • Plant growth bacterial agent and preparation method thereof
  • Plant growth bacterial agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) For the cultivation of test tube liquid strains, take fresh tofu waste water, boil for 10 minutes and naturally precipitate to get 100ml of supernatant, add (NH 4 ) 2 SO 4 0.1g, H 3 PO 4 0.05ml, after fully dissolving, adjust the pH to 6.2-7.2, pack in dry test tubes of 15×1.5cm, sterilize by autoclaving at 112°C for 30 minutes, cool to 32°C, inoculate F306 yeast fusion bacteria and colloid spores respectively Bacillus, Bacillus megaterium, Azotobacter rotundum, Trichoderma konshii in the test tube liquid culture medium, cultured at 28-32°C for 24-48 hours, it is the test tube liquid strain (first-class strain)

[0025] (2) The cultivation of Erlenmeyer flask liquid strain

[0026] Take fresh tofu waste water, boil for 10 minutes and naturally precipitate to get supernatant 1000ml, add (NH 4 ) 2 SO 4 1g, H 3 PO 40.5ml, after fully dissolving, adjust the pH to 6.2~7.2, put it in a 500ml Erlenmeyer flask, the filling amount is 1 / 4 of the total amount of the ...

Embodiment 2

[0032] (1) For the cultivation of test tube liquid strains, get 100ml of fresh vermicelli waste water, add (NH 4 ) 2 SO 4 0.1g, H 3 PO 4 0.05ml, after fully dissolving, adjust the pH to 6.2-7.2, divide into dry test tubes of 15×1.2cm, autoclave at 112°C for 30 minutes, cool to 32°C, inoculate F306 yeast fusion bacteria and colloid spores respectively Bacillus, Bacillus megaterium, Azotobacter rufica, Trichoderma konshii in the test tube liquid culture medium, cultured at 28-32°C for 24-48 hours, is the test tube liquid strain (first-class strain).

[0033] (2) The cultivation of Erlenmeyer flask liquid strain

[0034] Get fresh vermicelli waste water 1000ml, add (NH 4 ) 2 SO 4 1g, H 3 PO 4 0.5ml, after fully dissolving, adjust the pH to 6.2~7.2, put it in a 500ml Erlenmeyer flask, the filling amount is 1 / 4 of the total amount of the bottle, and then sterilize under high pressure at 112°C for 30 minutes, cool to 32°C, and put The test tube liquid strain is inoculate...

Embodiment 3

[0040] (1) For the cultivation of test tube liquid strains, fresh cornstarch wastewater was taken, and 100ml of supernatant was taken after boiling for 10 minutes to naturally precipitate, and added (NH 4 ) 2 SO 4 0.1g, H 3 PO 4 0.05ml, after fully dissolving, adjust the pH to 6.2-7.2, divide into dry test tubes of 15×1.2cm, autoclave at 112°C for 30 minutes, cool to 32°C, inoculate F306 yeast fusion bacteria and colloid spores respectively Bacillus, Bacillus megaterium, Azotobacter rufica, Trichoderma konshii in the test tube liquid culture medium, cultured at 28-32°C for 24-48 hours, is the test tube liquid strain (first-class strain).

[0041] (2) The cultivation of Erlenmeyer flask liquid strain

[0042] Get fresh cornstarch waste water, get supernatant 1000ml through boiling 10 minutes natural precipitation, add (NH 4 ) 2 SO 4 1g, H 3 PO 4 0.5ml, after fully dissolving, adjust the pH to 6.2~7.2, put it in a 500ml Erlenmeyer flask, the filling amount is 1 / 4 of t...

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Abstract

The invention relates to a plant growth bacterial agent of a microorganism and a preparation method thereof, which belong to the preparation technology of biological bacteria. The plant growth bacterial agent of the invention is mainly characterized by consisting of the following flora in percentage by weight: 25 to 40 percent of yeast fused strain, 15 to 30 percent of bacillus mucilaginosus, 15 to 30 percent of bacillus megaterium, 15 to 30 percent of azotobocter chroococcum and 15 to 30 percent of kahn trichoderma, and a liquid medium, wherein the collection number of the yeast fused strainis CGMCC 2461. The plant growth bacterial agent of the invention has the advantage that: experiments carried out by the inventor show that: the bacterial agent is applied during the growing period observation on a grey desert soil or saline alkali soil potted plant, so that the seedling emergence time, the seedling emergence rate, the trefoil stage and the seedling height of an experimental groupare all obviously improved compared with those of a control group. During the observation on roots of the grey desert soil or saline alkali soil potted plant, the root length, the root factor, the root volume and the fresh weight of the experimental group are obviously different from those of the control group.

Description

Technical field: [0001] The present invention mainly relates to a plant growth-assisting bacterial agent for microorganisms and a preparation method thereof. It belongs to the preparation technology of microbial bacterial agent. Background technique: [0002] There are a variety of microbial agents used for plant growth in China, such as Japan's EM, Shenli CM, VT, etc. , Trichoderma konshii composite method for preparing inoculum and the technology of inoculum that can help plants grow rapidly is still blank at present. Invention content: [0003] The purpose of the present invention is to avoid the deficiencies of the prior art and provide a plant growth-assisting bacterial agent and a preparation method thereof. Especially the method of compounding genetically engineered strains prepared by protoplast fusion technology with Bacillus colloidus, Bacillus megaterium, Azotobacter rufica and Trichoderma konii to prepare microbial inoculum. [0004] The raw material of the ...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P21/00C09K17/00C12N1/20C12N1/16C12N1/14C09K101/00C12R1/645C12R1/07C12R1/11C12R1/065C12R1/885
Inventor 孙荣高钟芳赵瑾
Owner 甘肃求实生物工程有限公司
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