Nonprogrammed cell frozen stock solution free of proteins
A cryopreservation solution and protein-free technology, applied in the field of protein-free cell cryopreservation solution, can solve the problems of inability to meet large batch processing, limited processing capacity, long cooling time, etc., and achieve good stability between batches and stable components Controllable, long-lasting effects
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Embodiment 1
[0036] The composition of the cell freezing solution is as follows:
[0037] Cell membrane protective agent 1%, composed of 0.5% sucrose, 0.5% high molecular weight anhydride-500, DMSO 8%,
[0038] Cell sedimentation stabilizer 25%, composed of methylcellulose 4000 CP 15%, hydroxyethyl starch 10%,
[0039] Antioxidant 0.2%, composed of Vitamin C 0.1%, Glutathione 0.1%,
[0040] Cell nutrient 1.0%, composed of glutamine 0.4%, sodium pyruvate 0.6%,
[0041] PBS buffer, balance.
Embodiment 2
[0043] The composition of the cell freezing solution is as follows:
[0044] Cell membrane protective agent 23%, composed of 11% trehalose, 5% panose, 7% middle molecular anhydride-70,
[0045] Permeable cell membrane inner protective agent 5%, composed of 2% propylene glycol and 3% glycerol,
[0046] Cell sedimentation stabilizer 8%, composed of 5% soluble starch, 3% dextrin,
[0047] Glutathione 0.3%,
[0048] Glutamine 0.3%
[0049] PBS buffer, balance.
Embodiment 3
[0051] The composition of the cell freezing solution is as follows:
[0052] Cell membrane protective agent 5%, composed of trehalose 2%, xylose 3%,
[0053] Permeable cell membrane inner protective agent 13%, composed of DMSO 5%, propylene glycol 8%,
[0054] Cell sedimentation stabilizer 20%, composed of methylcellulose 1500 CP 5%, hydroxyethyl starch 15%,
[0055] Antioxidant 0.1%, consisting of Vitamin C,
[0056] Cell Nutrition 0.5%, consisting of Sodium Pyruvate,
[0057] pH buffer, balance.
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