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Test strip for detecting HIV antibodies in spittle and preparation method thereof

A test strip and antibody technology, applied in the field of medical testing, can solve the problems of inconspicuous test results and low HIV antibody concentration in saliva, and achieve the effects of avoiding false negatives or missed tests, increasing test sensitivity, and directing the source.

Active Publication Date: 2013-04-03
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The purpose of the present invention is to solve the defects of low saliva HIV antibody concentration and inconspicuous detection results in the prior art, and provide a new test strip for detecting human immunodeficiency virus (HIV) antibodies in saliva

Method used

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  • Test strip for detecting HIV antibodies in spittle and preparation method thereof
  • Test strip for detecting HIV antibodies in spittle and preparation method thereof
  • Test strip for detecting HIV antibodies in spittle and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 The present invention detects the test strip of HIV antibody in saliva

[0046] Such as figure 1 Shown is a test strip for detecting HIV antibodies in saliva according to the present invention. Comprising a sample pad 1, a glass fiber membrane 2 tightly connected to one end of the sample pad 1 containing colloidal gold particle markers, a nitrocellulose membrane 3 closely connected to the other end of the glass fiber membrane 2, and the fiber The other end of the plain film 3 is closely connected to the absorbent paper 4, the sample pad 1, the glass fiber membrane 2, the nitrocellulose membrane 3 and the absorbent paper 4 are all pasted on the base plate 7, the sample pad 1 is a glass fiber membrane, The nitrocellulose membrane 3 includes a detection zone 5 coated with HIV recombinant antigens gp41 and gp36 and a control zone 6 coated with goat anti-rabbit IgG antibody, and the colloidal gold particle markers on the glass fiber membrane 2 include micro Si...

Embodiment 2

[0048] Embodiment 2 The preparation method of test strip of the present invention

[0049] Colloidal gold particle labeling is used in the present invention. Among them, the particle diameter of colloidal gold particles is nanometer (30-50nm). The adsorption mechanism of colloidal particles is to use its negatively charged properties under alkaline conditions to combine with positively charged groups of protein molecules by electrostatic attraction. Immunodiagnostic reagents. The preparation steps of the test strip for detecting human immunodeficiency virus (HIV) antibody in saliva are as follows:

[0050] 1. Preparation of anti-human IgG antibody

[0051] Isolate IgG from human cytoplasm by conventional methods, use human IgG to immunize mice, and after fusion of human IgG-immunized mice with myeloma cells, identify them by ELISA method, and culture the identified hybridomas in ascites, screen positive clones, and isolate Purified mouse anti-human IgG antibody. ELISA was ...

Embodiment 3

[0064] Embodiment 3 Utilize the test strip in embodiment 1 to detect the method for HIV antibody in saliva

[0065] In this example, the test strip of Example 1 is used to detect the HIV antibody in the saliva sample. For ease of use, an indicator arrow and a liquid level mark (MAX mark line) are pasted on the glass fiber membrane 2 .

[0066] Include the following steps:

[0067] (1) After collecting the saliva sample, add 1ml of sample treatment solution (0.02MPBS+0.05%Tween-20+1%BSA, pH7.4±0.2) for treatment and then use it for testing. At room temperature, samples should be processed within 1 hour of collection. If the sample cannot be tested in time after processing, the processed sample should be refrigerated at 2-8°C, and the sample should be tested within 12 hours after processing.

[0068] (2) Insert the sample pad 1 into the treated saliva sample solution in the direction of the arrow pointing down the test strip (the liquid level should not exceed the MAX mark li...

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Abstract

The invention discloses a test strip for detecting HIV antibodies in spittle and a preparation method thereof. The test strip comprises a sample pad, a fiberglass film, a nitrocellulose film and absorbent paper, wherein the fiberglass film is tightly connected with one end of the sample pad and contains colloidal gold particle markers; the nitrocellulose film is tightly connected with the other end of the fiberglass film; the absorbent paper is tightly connected with the other end of the nitrocellulose film; the sample pad, the fiberglass film, the nitrocellulose film and the absorbent paper are all arranged on a base plate; the nitrocellulose film comprises a detection area coated with HIV recombinant antigens and a control area coated with goat anti rabbit antibodies; and the colloidal gold particle marker comprises a colloidal gold particle-avidin-biotin-antihuman IgG antibody composite micro-signal amplification system, and colloidal gold marking rabbie IgG antibodies. Due to the adoption of the avidin-biotin micro-signal amplification system, the test strip amplifies signals of the target antibodies, improves detection sensitivity and avoids false negative or detection omission caused by too weak signals.

Description

technical field [0001] The invention belongs to the field of medical examination, in particular to a test strip for detecting human immunodeficiency virus (HIV) antibody in saliva and a preparation method thereof. Background technique [0002] Human immunodeficiency virus (Human Immunodeficiency Virus, HIV) is a virus that infects the cells of the human immune system. The virus destroys the immune system of the human body, causing the immune system to lose its resistance, and causing various diseases and cancers to survive in the human body , developed to the end, leading to AIDS (acquired immunodeficiency syndrome). AIDS is a deadly infectious disease for which there is no effective treatment so far. [0003] The cost of treatment for AIDS patients is high. The medical expenses of the first batch of AIDS patients in the United States were US$1.5 billion; Chinese experts estimate that the direct and indirect cost of each AIDS patient is about 150,000 yuan. The investment i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/544
Inventor 王继华
Owner GUANGZHOU WONDFO BIOTECH
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